Synopsis
Newly-hatched embryos of Oreochromis mossambicus were reared in freshwater and treated with 0 (control), 50 (low level) or 200 (high level) ppb cadmium for 4 days. Changes in the numbers and dimensions of chloride cell apical crypts on the skin of the free embryos were examined daily using scanning electron microscopy. The apical crypts of the chloride cells were rarely observed on the skin of the embryo trunk, and unevenly distributed on the surface of the yolksac. Two days after hatching, the chloride cells of the free embryos exposed to 50 ppb Cd were more ‘active’ than those of the other two groups. Compared with the control group, the maximum dimensions of the developing apical crypts were stimulated by 50 ppb Cd and inhibited by 200 ppb Cd. The results indicated that the development of chloride cells in tilapia free embryos was provoked by low level Cd exposure and stunted by high level Cd exposure, suggesting the existence of structure/function relationships in which the activation of chloride cells may be related to the ionoregulatory mechanism in adaptation to Cd exposure.
References cited
Alderdice, d.F. 1988. Osmotic and ionic regulation in teleost eggs and larvae. pp. 163–252. In: W.S. Hoar & D.J. Randall (ed.) Fish Physiology, Vol. 11A, Academic Press, New York.
Brown, P. 1992. Gill chloride cell surface-area is greater in freshwater-adapted adult sea trout (Salmo trutta) than those adapted to sea water. J. Fish Biol. 40: 481–484.
Depeche, J. 1973. Ultrastructure of the yolks and pericardial sac surface in the embryo of teleost Poecilia reticulata. Z. Zellforsch. Mikrosk. Anat. 141: 236–253.
Fu, H., R.A.C. Lock & S.E. Wendelaar Bonga. 1989. Effect of cadmium on prolactin cell activity and plasma electrolytes in the freshwater teleost Oreochromis mossambicus. Aquat. Toxicol. 14: 295–306.
Guggino, W.B. 1980a. Water balance in embryos of Fundulus heteroclitus and F. bermudae adapted to sea water. Amer. J. Physiol. 238R: 36–41.
Guggino, W.B. 1980b. Salt balance in embryos of Fundulus heteroclitus and F. bermudae adapted to sea water. Amer. J. Physiol. 238R: 42–61.
Heath, A.G. 1987. Osmotic and ionic regulation. pp. 107–130. In: A.G. Heath (ed.) Water Pollution and Fish Physiology, CRC Press, Boca Raton.
Holden, K.K. & M.N. Bruton. 1992. A life-history approach to the early ontogeny of the Mozambique tilapia Oreochromis mossambicus (Pisces, Cichlidae). S. Afr. J. Zool. 27: 173–191.
Hossler, F.E., G. Musil & L.J. Karnaky, Jr. 1985. Surface ultrastructure of the gill arch of the killifish, Fundulus heteroclitus, from seawater and freshwater, with special reference to the morphology of apical crypts of chloride cells. J. Morphol. 185: 377–386.
Hwang, P.P. 1988. Multicellular complex of chloride cells in the gills of freshwater teleosts. J. Morphol. 196: 15–22.
Hwang, P.P. 1989. Distribution of chloride cells in teleost larvae. J. Morphol. 200: 1–8.
Hwang, P.P. 1990. Salinity effects of development of chloride cells in the larvae of ayu (Plecoglossus altivelis). Mar. Biol. 107: 1–7.
Hwang, P.P. & R. Hirano. 1985. Effects of environmental salinity on intercellular organization and junctional structure of chloride cells in early stages of teleost development. J. Exp. Zool. 236: 115–126.
Hwang, P.P. & Y.N. Tsai. 1993. Effects of arsenic on osmoregulation in the tilapia Oreochromis mossambicus reared in seawater. Mar. Biol. 117: 551–558.
Hwang, P.P., S.W. Lin & H.C. Lin.1995. Different sensitivities to Cadmium in tilapia larvae (Oreochromis mossambicus; Teleostei). Arch. Environ. Contam. Toxicol. (in press).
Karnaky, Jr., K.J. 1986. Structure and function of the chloride cell of Fundulus heteroclitus and other teleosts. Amer. Zool. 26: 209–224.
King, J.A.C. & F.E. Hossler. 1991. The gill arch of the striped bass (Morone saxatilis). IV Alterations in the ultrastructure of chloride cell apical crypts and chloride efflux following exposure to seawater. J. Morphol. 209: 165–167.
Lasker, R. & L.T. Threadgold. 1968. "Chloride cells" in the skin of the larval sardine. Exp. Cell Res. 52: 582–590.
Laurent, P & S.F. Perry. 1991. Environmental effects on fish gill morphology. Physiol. Zool. 64: 4–25.
Mallatt, J. 1985. Fish gill structural changes induced by toxicants and other irritants: a statistical review. Can. J. Fish. Aquat. Sci. 42: 630–648.
McDonald, D.G. & C.M. Wood. 1993. Branchial mechanisms of acclimation to metals in freshwater fish. pp. 297–321. In: J.C. Rankin & F.B. Jensen (ed.) Fish Ecophysiology, Chapman & Hall, London.
O'Connell, C.P 1981. Development of organ systems in the northern anchovy Engraulis mordax and other teleosts. Amer. Zool. 21: 429–446.
Oladimeji, A.A., S.U. Qadri & S.W. deFreitas. 1984. Measuring the elimination of arsenic by the gills of rainbow trout (Salmo gairdneri) by using a two compartment respirometer. Bull. Environ. Contam. Toxic. 32: 661–668.
Oronsaye, J.A.O. & A.E. Brafield. 1984. The effect of dissolved cadmium on the chloride cells of the gills of the stickleback, Gasterosteus aculeatus L. J. Fish. Biol. 25: 253–258.
Pascoe, D., S.A. Evans & J. Woodward. 1986. Heavy metal toxicity to fish and the influence of water hardness. Arch. Environ. Contam. Toxicol. 15: 481–487.
Pereira, J.T. 1988. Morphological effects of mercury exposure on flounder gills as observed by scanning electron microscopy. J. Fish Biol. 33: 571–580.
Perry, S.F. & P. Laurent. 1993. Environmental effects on fish gill structure and function. pp. 231–264. In: J.C. Rankin & F.B. Jensen (ed.) Fish Ecophysiology, Chapman & Hall, London.
Pratap, H.B. & S.E. Wendelaar Bonga. 1993. Effect of ambient and dietary cadmium on pavement cells, chloride cells, and Na+/K+-ATPase activity in the gills of the freshwater teleost Oreochromis mossambicus at normal and high calcium levels in the ambient water. Aquat. Toxicol. 26: 133–150.
Reid, S.D. & D.G. McDonald. 1988. Effects of cadmium, copper, and low pH on ion fluxes in the rainbow trout, Salmo gairdneri. Can. J. Fish. Aquat. Sci. 45: 244–253.
Rombough, P.J. & E.T. Garside. 1982. Cadmium toxicity and accumulation in eggs and alevins of Atlantic salmon Salmo salar. Can. J. Zool. 60: 2006–2014.
Somasundaram, B. 1985. Effects of zinc on epidermal ultrastructure in the larva of Clupea harengus. Mar. Biol. 85: 199–207.
Spry, D.J. & C.M. Wood. 1985. Ion flux rates, acid-base status, and blood gases in rainbow trout, Salmo gairdneri, exposed to toxic zinc in natural soft water. Can. J. Fish. Aquat. Sci. 42: 1332–1341.
Weisbart, M. 1968. Osmotic and ionic regulation in embryos, alevin and fry of the five species of Pacific salmon. Can. J. Zool. 163: 237–264.
Wendelaar Bonga, S.E. & R.A.C. Lock. 1992. Toxicants and osmoregulation in fish. Netherlands J. Zool. 42: 478–493.
Westernhagen, H. von. 1988. Sublethal effects of pollutants on fish eggs and larvae. pp. 253–346. In: W.S. Hoar & D.J. Randall (ed.) Fish Physiology, Vol. 11A, Academic Press, New York.
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Lee, TH., Hwang, PP. & Lin, HC. Morphological changes of integumental chloride cells to ambient cadmium during the early development of the teleost, Oreochromis mossambicus . Environ Biol Fish 45, 95–102 (1996). https://doi.org/10.1007/BF00000631
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DOI: https://doi.org/10.1007/BF00000631