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Recombinant xylanase production by Escherichia coli using a non-induced expression system with different nutrient sources

  • Elenira H. M. Mendonça
  • Nilton Cesar Avanci
  • Luis Henrique Romano
  • Daniel Lopes Branco
  • Alessandra Xavier de Pádua
  • Richard John Ward
  • Álvaro de Baptista NetoEmail author
  • Marcos Roberto Lourenzoni
Original Paper

Abstract

The application of enzymes for sustainable and low-environmental impact industrial processes requires high-level enzyme production at low-cost. A promising strategy is the use of a high efficiency heterologous protein expression system using E. coli and the pT7BsXA vector encoding the GH11 xylanase from Bacillus subtilis with promoter, replication origin and signal peptide sequences from B. subtilis (Ruller et al. 2006). This expression system produces high amounts of enzyme that are secreted to the culture broth. The present study aimed to maximize the xylanase production by this system through evaluation of culture medium composition. Different culture media previously described in the literature together with compositions derived from agro-industrial residues were evaluated. A culture medium derived from agro-industrial residues using sugarcane molasses as carbon source showed a 9-fold increase in enzyme production (195,000 U/L) in relation to LB medium and the lowest production cost, which was 8.5-fold lower than LB medium using sugarcane molasses as carbon source and brewer’s yeast as vitamin source in shaker experiments. In a bioreactor experiment the best production medium promoted an 8.5-fold higher production at a 10.8-fold lower cost as compared to shaker LB cultivation.

Keywords

Xylanase Recombinant enzymes Bioprocess Agro-industrial residues E. coli 

Notes

Acknowledgements

We would like to thank FAPESP (Proc. 2008/53426-7 and 2010/50328-4) and CNPq (stipends to E.H.M.M and N.C.A.) for financial support.

Supplementary material

43153_2019_4_MOESM1_ESM.docx (20 kb)
Supplementary material 1 (DOCX 20 kb)

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Copyright information

© Associação Brasileira de Engenharia Química 2020

Authors and Affiliations

  • Elenira H. M. Mendonça
    • 1
  • Nilton Cesar Avanci
    • 1
  • Luis Henrique Romano
    • 1
  • Daniel Lopes Branco
    • 1
  • Alessandra Xavier de Pádua
    • 1
  • Richard John Ward
    • 2
  • Álvaro de Baptista Neto
    • 3
    Email author
  • Marcos Roberto Lourenzoni
    • 1
    • 4
  1. 1.Verdartis Desenvolvimento Biotecnológico LtdaRibeirão PretoBrazil
  2. 2.Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil
  3. 3.Departamento de Bioprocessos e Biotecnologia, Faculdade de Ciências FarmacêuticasUniversidade Estadual Paulista Júlio de Mesquita FilhoAraraquaraBrazil
  4. 4.Fundação Oswaldo Cruz, FiocruzEusébioBrazil

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