Selection of yeasts from bee products for alcoholic beverage production

  • Mayara Salgado Silva
  • Luciana Marina Arruda
  • Pedro Lanna Xavier
  • Maria Ximena Díaz Ramírez
  • Fernando Augusto da Silveira
  • Weyder Cristiano Santana
  • Paulo Henrique Alves da Silva
  • Luciano Gomes Fietto
  • Monique Renon EllerEmail author
Food Microbiology - Research Paper


The use of appropriate yeast strains allows to better control the fermentation during beverage production. Bee products, especially of stingless bees, are poorly explored as sources of fermenting microorganisms. In this work, yeasts were isolated from honey and pollen from Tetragonisca angustula (Jataí), Nannotrigona testaceicornis (Iraí), Frieseomelitta varia (Marmelada), and honey of Apis mellifera bees and screened according to morphology, growth, and alcohol production. Bee products showed to be potential sources of fermenting microorganisms. From 55 isolates, one was identified as Papiliotrema flavescens, two Rhodotorula mucilaginosa, five Saccharomyces cerevisiae, and nine Starmerella meliponinorum. The S. cerevisiae strains were able to produce ethanol and glycerol at pH 4.0–8.0 and temperature of 10–30 °C, with low or none production of undesirable compounds, such as acetic acid and methanol. These strains are suitable for the production of bioethanol and alcoholic beverages due to their high ethanol production, similar or superior to the commercial strain, and in a broad range of conditions like as 50% (m/v) glucose, 10% (v/v) ethanol, or 500 mg L−1 of sodium metabisulfite.


Ethanol production Yeast selection Stingless bees Honey 


Funding information

This work was supported by Fundação Arthur Bernardes (FUNARBE), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Compliance with ethical standards


The funders had no role in the study design, data collection, analysis, publishing decisions, or preparation of this manuscript.


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Copyright information

© Sociedade Brasileira de Microbiologia 2019

Authors and Affiliations

  • Mayara Salgado Silva
    • 1
  • Luciana Marina Arruda
    • 2
  • Pedro Lanna Xavier
    • 2
  • Maria Ximena Díaz Ramírez
    • 2
  • Fernando Augusto da Silveira
    • 3
  • Weyder Cristiano Santana
    • 4
  • Paulo Henrique Alves da Silva
    • 2
  • Luciano Gomes Fietto
    • 5
  • Monique Renon Eller
    • 2
    Email author
  1. 1.Instituto Federal do CearáLimoeiro do NorteBrazil
  2. 2.Department of Food TechnologyUniversidade Federal de ViçosaViçosaBrazil
  3. 3.Department of MicrobiologyUniversidade Federal de ViçosaViçosaBrazil
  4. 4.Department of EntomologyUniversidade Federal de ViçosaViçosaBrazil
  5. 5.Department of Biochemistry and Molecular BiologyUniversidade Federal de ViçosaViçosaBrazil

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