High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil

  • Felipe Pinheiro Vilela
  • Dália dos Prazeres Rodrigues
  • Renata Garcia Costa
  • Monique Ribeiro Tiba Casas
  • Juliana Pfrimer FalcãoEmail author
  • Fábio CampioniEmail author
Bacterial Fungal and Virus Molecular Biology - Research Paper


Salmonella Dublin is a strongly adapted serovar that causes enteritis and/or systemic disease with high rates of mortality in cattle and occasionally infects humans. Despite the importance of this serovar, there is a lack of studies in Brazil. The aim of this study was to characterize the genetic diversity of 112 S. Dublin strains isolated from humans and animals in Brazil by CRISPR and CRISPR-MVLST and the relatedness among strains by MLST. In addition, the frequency of some important virulence genes was verified. The strains studied belonged to nine different sequence types, being all of them single- or double-locus variants of the ST10. CRISPR discriminated the strains into 69 subtypes with a similarity ≥ 84.4% and CRISPR-MVLST into 72 subtypes with a similarity ≥ 84.7%. The virulence genes ratB, lpfA, mgtC, avrA, sopB, sopE2, sifA, sseA, ssrA, csgA, fliC, and sinH were found in all the strains studied, while spvB, spvC, sodCl, rpoS, sipA, sipD, invA, and hilA were detected in ≥ 93.7% of the strains. In conclusion, the high similarity among the strains reinforces the clonal nature of the strains of this serovar that may have descended from a common ancestor that little differed over 33 years in Brazil. CRISPR and CRISPR-MVLST showed to be good alternatives to type S. Dublin strains. MLST suggested that S. Dublin strains from Brazil were phylogenetically related to strains from other parts of the globe. Moreover, the high frequency of virulence genes among the strains studied reinforces the capacity of S. Dublin to cause invasive diseases.


Salmonella Dublin MLST CRISPR CRISPR-MVLST Virulence genes 



The authors thank São Paulo Research Foundation - FAPESP for financial support and Dr. Marc Allard from FDA/CFSAN for providing whole-genome sequencing of the strains studied.

Funding information

This study was financed by São Paulo Research Foundation (FAPESP) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. During the course of the work, F.P. Vilela was supported by a scholarship from FAPESP (Proc. 2017/05756-7 and 2019/06947-6) and F. Campioni was supported by a postdoctoral fellowship from FAPESP (Proc. 2013/25191-3). Part of this work was also financed by a FAPESP grant (Proc. 2016/24716-3) under the supervision of J.P. Falcão that also received a productive fellowship (CNPq 303475/2015-3 and CNPq 304399/2018-3) from National Council for Scientific and Technological Development (CNPq).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

42770_2019_156_MOESM1_ESM.docx (14 kb)
ESM 1 (DOCX 13 kb)


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Copyright information

© Sociedade Brasileira de Microbiologia 2019

Authors and Affiliations

  1. 1.Departamento de Análises Clínicas, Toxicológicas e BromatológicasFaculdade de Ciências Farmacêuticas de Ribeirão Preto – USPRibeirão PretoBrazil
  2. 2.Fundação Oswaldo Cruz – FIOCRUZ, Pavilhão Rocha LimaRio de JaneiroBrazil
  3. 3.Centro de BacteriologiaInstituto Adolfo LutzSão PauloBrazil

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