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Antibiotic resistance in Salmonella enterica isolated from dairy calves in Uruguay

  • M. L. Casaux
  • R. D. Caffarena
  • C. O. Schild
  • F. Giannitti
  • Franklin Riet-Correa
  • Martín FragaEmail author
Veterinary Microbiology - Short Communication

Abstract

Salmonella enterica is an important animal and human pathogen that can cause enteritis and septicaemia in calves. Generally, antibiotics are prescribed for the treatment of salmonellosis in dairy calves. Here, we report the isolation of antibiotic resistant S. enterica serotypes from calves, including multidrug-resistant isolates. A total of 544 faecal samples from live healthy and diarrheic dairy calves from 29 commercial dairy farms and organ samples from 19 deceased calves that succumbed to salmonellosis in 12 commercial dairy farms in Uruguay were processed for selective S. enterica culture. In total, 41 isolates were serotyped, and susceptibility to 14 antibiotics, from 9 classes of compounds, was evaluated by disk-diffusion test. The minimum inhibitory concentration (MIC) was determined by microdilution. Salmonella Typhimurium was the most frequent serotype, followed by S. Dublin and S. Anatum. Whether determined by diffusion assay or microdilution, resistance to tetracycline, streptomycin and ampicillin were the most frequently pattern found. Based on MIC, 5 isolates were resistant to at least one antibiotic, 21 were resistant to 2 antibiotics, and 14 were multidrug-resistant (resistant to at least one antibiotic in 3 different categories of antibiotics). Eleven different resistance patterns were found. Multidrug resistance in S. enterica is a concern for animal and public health not only because of its zoonotic potential but also due to the possibility of transfer resistance determinants to other bacterial genera. This represents the first report of the antibiotic resistance in S. enterica in dairy farms in Uruguay.

Keywords

Salmonella Typhimurium Salmonella Dublin Salmonella Anatum Antibiotic resistance Dairy calves 

Notes

Acknowledgements

The authors thank Cecilia Monesiglio from INIA for technical assistance with microbiological routine, and Laura Betancor and Arací Martínez from the “Instituto de Higiene, Facultad de Medicina, UdelaR” for Salmonella serotyping.

Data availability statement

The datasets generated during and/or analysed during the current study are available from the corresponding author on request.

Funding information

This work was partially funded by INIA’s grant PL-15, and project FMV-104922 from the Uruguayan “Agencia Nacional de Investigación e Innovación” (ANII). M.L. Casaux received master’s scholarships from ANII (POS_NAC_2016_1_130296).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

42770_2019_151_MOESM1_ESM.docx (18 kb)
Table S1 (DOCX 18 kb)

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Copyright information

© Sociedade Brasileira de Microbiologia 2019

Authors and Affiliations

  1. 1.Instituto Nacional de Investigación Agropecuaria (INIA), Plataforma de Investigación en Salud AnimalEstación Experimental INIA La EstanzuelaColoniaUruguay
  2. 2.Facultad de VeterinariaUniversidad de la RepúblicaMontevideoUruguay

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