This study explores the potential of lead resistant bacterium Acinetobacter junii Pb1 for adsorption/accumulation of lead using various techniques. In the present work, growth of A. junii Pb1 was investigated in the presence of a range of Pb(II) concentrations (0, 100, 250, 500, and 1000 mg l−1). Lead was found to have no toxic effect on the growth of A. junii Pb1 at 100 and 250 mg l−1 concentrations. However, further increase in Pb(II) concentration (500 mg l−1) showed increase in lag phase, though growth remained unaffected and significant growth inhibition was observed when concentration was increased to 1000 mg l−1. Same was confirmed by the observations of flow cytometry. Further, the effect of Pb(II) on A. junii Pb1 was evaluated by using fluorescence microscopy, spectrofluorimetry, and flow cytometry. The spectrofluorimetry and fluorescence microscopy results revealed the accumulation of Pb(II) inside the bacterial cells as evident by green fluorescence due to lead binding fluorescent probe, Leadmium Green AM dye. Flow cytometry observations indicate an increase in cell size and granularity of exposure to lead. Thus, present work provides a new understanding of Pb(II) tolerance in A. junii Pb1 and its potential use in remediation of lead from contaminated soil.
Lead Acinetobacter juniiCytotoxicity Propidium iodide Leadmium Green AM dye
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The authors are grateful to MNNIT, Allahabad for this research. The authors are grateful to CIR, MNNIT, Allahabad and CMDR, MNNIT, Allahabad for providing facility for spectrofluorimetry and flow cytometry respectively. The authors are grateful to Dr. Jyotsna Sinha for the proofread for English language and grammar.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Bind A, Goswami L, Prakash V (2018) Comparative analysis of floating and submerged macrophytes for heavy metal (copper, chromium, arsenic and lead) removal: sorbent preparation, characterization, regeneration and cost estimation. Geol Ecol Landsc 2(2):61–72.CrossRefGoogle Scholar
Arul Manikandan N, Alemu AK, Goswami L, Pakshirajan K, Pugazhenthi G (2016) Waste litchi peels for Cr (VI) removal from synthetic wastewater in batch and continuous systems: sorbent characterization, regeneration and reuse study. J Environ Eng 142(9):C4016001CrossRefGoogle Scholar
Kushwaha A, Rani R, Kumar S, Gautam A (2015) Heavy metal detoxification and tolerance mechanisms in plants: implications for phytoremediation. Environ Rev 23:1–13CrossRefGoogle Scholar
Kushwaha A, Rani R, Kumar S (2017) Mechanism of soil-metal-microbe interactions and their implication on microbial bioremediation and phytoremediation. In book: Environmental science and engineering volume 8 biodegradation and bioremediation edition: edition 1st volume 8, Publisher: Studium press LLC, U.S.A. Editors: Pravinder Kumar, Bhola R Gurjar, J N GovilGoogle Scholar
Kushwaha A, Hans N, Rani R, Kumar S (2018) A critical review on speciation, mobilization and toxicity of lead in soil-microbe-plant system and bioremediation strategies. Ecotoxicol Environ Saf 147:1035–1045CrossRefGoogle Scholar
Goswami L, Manikandan NA, Pakshirajan K, Pugazhenthi G (2017) Simultaneous heavy metal removal and anthracene biodegradation by the oleaginous bacteria Rhodococcus opacus. 3 Biotech 7(1):37CrossRefGoogle Scholar
Gupta DK, Huang HG, Yang XE, Razafindrabe BHN, Inouhe M (2010) The detoxification of lead in Sedum alfredii H. is not related to phytochelatins but the glutathione. J Hazard Mater 177:437–444CrossRefGoogle Scholar
Maestri E, Marmiroli M, Visioli G, Marmiroli N (2010) Metal tolerance and hyperaccumulation: costs and trade-offs between traits and environment. Environ Exp Bot 68:1–13CrossRefGoogle Scholar
Ghosh M, Singh S (2005) A review on phytoremediation of heavy metals and utilization of it’s by products. Asian J Energy Environ 6(4):18Google Scholar
Glick BR (2010) Using soil bacteria to facilitate phytoremediation. Biotechnol Adv 28:367–374CrossRefGoogle Scholar
Ali H, Khan E, Sajad MA (2013) Phytoremediation of heavy metals-concepts and applications. Chemosphere 91:869–881CrossRefGoogle Scholar
Kushwaha A, Rani R, Kumar S, Thomas T, David AA, Ahmed M (2017) A new insight to adsorption and accumulation of high lead concentration by exopolymer and whole cells of lead-resistant bacterium Acinetobacter junii L. Pb1 isolated from coal mine dump. Environ Sci Pollut Res 24:10652–10661CrossRefGoogle Scholar
Naik MM, Dubey SK (2013) Lead resistant bacteria: lead resistance mechanisms, their applications in lead bioremediation and biomonitoring. Ecotoxicol Environ Saf 98:1–7CrossRefGoogle Scholar
Borremans B, Hobman JL, Provoost A, Brown NL, van der Lelie D (2001) Cloning and functional analysis of the pbr lead resistance determinant of Ralstonia metallidurans CH34. J Bacteriol 183:5651–5658CrossRefGoogle Scholar
Park JH, Bolan N, Megharaj M, Naidu R (2011) Concomitant rock phosphate dissolution and lead immobilization by phosphate solubilizing bacteria (Enterobacter sp.). J Environ Manag 92:1115–1120CrossRefGoogle Scholar
Jaafar R, Al-Sulami A, Al-Taee A (2016) Bioaccumulation of cadmium and lead by Shewanella oneidensis isolated from soil in Basra governorate, Iraq. Afr J Microbiol Res 10:370–375CrossRefGoogle Scholar
Malaiyandi LM, Sharthiya H, Dineley KE (2016) Fluorescence detection of intracellular cadmium with Leadmium Green. Biometals 29:625–635CrossRefGoogle Scholar
Marzan LW, Hossain M, Mina SA, Akter Y, Chowdhury AMA (2017) Isolation and biochemical characterization of heavy-metal resistant bacteria from tannery effluent in Chittagong city, Bangladesh: bioremediation viewpoint. Egypt J Aquat Res 43(1):65–74CrossRefGoogle Scholar
Chen Z, Pan X, Chen H, Lin Z, Guan X (2015) Investigation of lead (II) uptake by Bacillus thuringiensis 016. World J Microbiol Biotechnol 31(11):1729–1736CrossRefGoogle Scholar
Chatterjee S, Das J, Chatterjee S, Choudhuri P, Sarkar A (2014) Isolation, characterization and protein profiling of Lead resistant Bacteria. Br Microbiol Res J 4(1):116–131CrossRefGoogle Scholar
Khan Z, Rehman A, Hussain SZ, Nisar MA, Zulfiqar S, Shakoori AR (2016) Cadmium resistance and uptake by bacterium, Salmonella enterica 43C, isolated from industrial effluent. AMB Express 6(1):54CrossRefGoogle Scholar
Jobby R, Jha P, Desai N (2015) Sinorhizobium, a potential organism for bioremediation of nickel. Int J Adv Res 3:706–717Google Scholar