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Analysis of culturable microbiota present in the stomach of children with gastric symptoms

  • Changcheng Guo
  • Fang Liu
  • Li Zhu
  • Fangcao Wu
  • Guzhen Cui
  • Yan Xiong
  • Qiong Wang
  • Lin Yin
  • Caixia Wang
  • Huan Wang
  • Xiaojuan Wu
  • Zhengrong Zhang
  • Zhenghong ChenEmail author
Clinical Microbiology - Research Paper

Abstract

Despite extensive studies on the gastric microbiota, including Helicobacter pylori and non-H. pylori, the bacterial composition in children remains unknown. In this study, we analyzed the culturable gastric bacteria in stomach biopsies from 346 children aged 1–15 years affected by gastric diseases. H. pylori and non-H. pylori were identified by specific PCR and 16S rDNA sequencing, respectively. Antibiotic susceptibilities of H. pylori and non-H. pylori were tested by the E-test and disk diffusion methods, respectively. Rapid diagnosis was also performed by H. pylori–specific PCR. Twenty-two H. pylori strains were obtained from culture, and 92 biopsies were positive by H. pylori–specific PCR. The positive rate was higher in boys (40.3%) than in girls (23.3%) (P = 0.001). Resistance rates of 22 H. pylori strains were as follows: metronidazole, 86.4%; tetracycline, 22.7%; amoxicillin, 22.7%; levofloxacin, 31.8%; clarithromycin, 36.4%. Ten isolates were multidrug-resistant. Additionally, among 366 non-H. pylori strains, 204 exhibited urease activity. Non-H. pylori resistance rates were as follows: metronidazole, 94.8%; tetracycline, 26.2%; amoxicillin, 42.6%; levofloxacin, 15.3%; clarithromycin, 46.7%. Our results showed that children with gastric disorders harbor stomach bacteria with urease activity or nitrate reductase activity. Further studies will determine the effects of non-H. pylori bacteria in gastric diseases.

Keywords

Helicobacter pylori Gastric microbiota Urease Resistance 

Notes

Acknowledgements

We would like to thank Editage [www.editage.cn] for the English language editing.

Funding

This work was supported by the National Natural Science Foundation of China (No. 81460314 and 3176080034), Foundation of Education Department of Guizhou (Project number: KY-2014-216), and Foundation of Guiyang Health and Family Planning Commission (No. 2014-018).

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Sociedade Brasileira de Microbiologia 2018

Authors and Affiliations

  • Changcheng Guo
    • 1
  • Fang Liu
    • 1
  • Li Zhu
    • 2
  • Fangcao Wu
    • 1
  • Guzhen Cui
    • 1
  • Yan Xiong
    • 2
  • Qiong Wang
    • 1
  • Lin Yin
    • 1
  • Caixia Wang
    • 1
  • Huan Wang
    • 1
  • Xiaojuan Wu
    • 1
  • Zhengrong Zhang
    • 1
  • Zhenghong Chen
    • 1
    • 3
    Email author
  1. 1.School of Basic Medical Science, Key Laboratory of Medical microbiology and parasitology of Education Department of GuizhouGuizhou Medical UniversityGuiyangChina
  2. 2.Clinical Medical School of Maternal and Child Affiliated to Guizhou Medical UniversityGuiyangChina
  3. 3.Department of Microbiology, School of Basic Medical ScienceGuizhou Medical UniversityGuiyangChina

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