The metabolism and genetic regulation of lipids in the oleaginous yeast Yarrowia lipolytica
- 13 Downloads
The biotechnological potential of Yarrowia lipolytica, as a single cell oil-producing microorganism, is presented in this review. Although initially this yeast species was considered as a lipid-degrading, recently, it was reclassified as a lipid-producing microorganism, since it has been reported to be capable of accumulating diverse desirable fatty acids after metabolic pathway engineering. In the first part of the present document, a general revision of the oil metabolic pathways and the capacity of oil production in Y. lipolytica is presented. The single cell oil produced by these metabolic engineering strategies has been designed by optimization, introduction, or suppression of new pathways to increase yield on lipid production. Later on, the genetic regulation systems and the lipid composition generated by this yeast for industrial purposes are discussed. These lipids could be safely used in the chemical food and biofuel industries, due to their high proportion of oleic acid. This document emphasizes in the overviewing at Y. lipolytica as an ideal oil cell factory, and as an excellent model to produce single cell oil.
KeywordsLipid metabolism Citric acid Lipases Hydrophobic substrates Y. lipolytica
The authors thanks to the Mexican Instituto Politécnico Nacional, through SIP funding, for its contribution in the knowledge generation about this microorganism. Likewise, we are grateful for the scholarships granted by CONACYT Mexico.
Compliance with ethical standards
Conflict of interest
The authors declare not having a conflict of interests in this article’s publishing.
- 1.Thorsten Lumbsch H, Huhndorf SM (2007) Outline of Ascomycota 2007. Myconet 13:1–58Google Scholar
- 6.Rosas-Quijano R, Gallardin C (2011) Sistema Cre/loxP1 como herramienta genética en Yarrowia lipolytica. Rev Mex Micol 33:17–27Google Scholar
- 40.Tsigie YA, Wang CY, Kasim NS, Diem QD et al (2012) Oil production from Yarrowia lipolytica Po1g using rice bran hydrolysate. J Biomed Biotechnol:1–10. https://doi.org/10.1155/2012/378384
- 41.Gonçalves FAG, Colen G, Takahashi JA (2014) Yarrowia lipolytica and its multiple applications in the biotechnological industry. Scientific World J:1–14. https://doi.org/10.1155/2014/476207
- 45.Blazeck J, Hill A, Liu L, Knight R et al (2014) Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production. Nature Comm 31:1–10Google Scholar
- 46.Fukuda R (2013) Metabolism of hydrophobic carbon sources and regulation of it in n-alkane-assimilating yeast Yarrowia lipolytica. Biosci Biotechnol Biochem (6):1149–1154Google Scholar
- 48.Dulermo T, Tréton B, Beopoulos A, Kabran APG et al (2013) Characterization of the two intracellular lipases of Y. lipolytica encoded by TGL3 and TGL4 genes: new insights into the role of intracellular lipases and lipid body organization. Biochim Biophys Acta 1831:482–491Google Scholar