Fungal Endophyte Phomopsis liquidambari Improves Iron and Molybdenum Nutrition Uptake of Peanut in Consecutive Monoculture Soil
Iron (Fe) and molybdenum (Mo) are essential trace elements for plants, especially for peanut. However, peanut planted in consecutive monoculture soil usually suffers from malnutrition. Thus, the objective of this study was to investigate the effects of the fungal endophyte Phomopsis liquidambari on Fe and Mo nutrition uptake of peanut in consecutive monoculture soil. Plant growth parameters, physiological parameters, the accumulation of Fe and Mo elements, gene expression related to absorption and transformation of Fe and Mo in planta, and the low-molecular-weight organic acids (LMWOAs) concentration in rhizosphere soil were analyzed by greenhouse pot experiments. The results showed that the application of P. liquidambari improved growth parameters, chlorophyll content, nitrate reductase (NR) activity, and Fe and Mo nutrition of peanut. Furthermore, LMWOAs in peanut rhizosphere soil and the expression levels of AhFRO1, AhIRT1, and AhMOT1 were significantly changed by the application of fungal endophyte. These findings indicated that the improvement of Fe and Mo nutrition in peanut was mostly derived from the promoting growth function of P. liquidambari and the regulation of peanut-P. liquidambari symbiosis with rhizosphere LMWOAs and Fe- and Mo-related gene expression in continuous monoculture soil.
KeywordsFungal endophyte Peanut Iron Molybdenum Nutrition uptake Consecutive monoculture soil
This work was supported by the National Natural Science Foundation of China (NSFC) under Grant number 31870478 and the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions of China.
- Bastani S, Hajiboland R, Khatamian M, Saket-Oskoui M (2018) Nano iron (Fe) complex is an effective source of Fe for tobacco plants grown under low Fe supply. J Soil Sci Plant Nutr. https://doi.org/10.4067/S0718-95162018005001602
- Hong Y (2012) The relationship between root traits and aboveground traits in peanut (Arachis hypogaea L.). Afr J Agric Res 7:6234–6238Google Scholar
- Keable SM, Vertemara J, Zadvornyy OA, Eilers BJ, Danyal K, Rasmussen AJ, De Gioia L, Zampella G, Seefeldt LC, Peters JW (2017) Structural characterization of the nitrogenase molybdenum-iron protein with the substrate acetylene trapped near the active site. J Inorg Biochem 180:129–134CrossRefGoogle Scholar
- Li PD, Dai CC, Wang XX, Zhang TL, Chen Y (2012) Variation of soil enzyme activities and microbial community structure in peanut monocropping system in subtropical China. Afr J Agric Res 7:219–228Google Scholar
- Long WJ, Gu T, Wan NX, Peng BY, Kong FL, Yuan JC (2017) Effect of low iron stress on root growth and iron uptake and utilization of different maize cultivars at seedling stage. Chin J Eco-Agric 25:1163–1172Google Scholar
- Nie ZJ, Hu CX, Tan QL, Sun XC (2016) Gene expression related to molybdenum enzyme biosynthesis in response to molybdenum deficiency in winter wheat. J Soil Sci Plant Nutr 16:979–990Google Scholar
- Pizarro C, Escudey M, Gacitua MA, Fabris JD (2018) Iron-bearing minerals from soils developing on volcanic materials from Southern Chile: application in heterogeneous catalysis. J Soil Sci Plant Nutr. https://doi.org/10.4067/S0718-95162018005002001
- Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384–394CrossRefGoogle Scholar
- Shi Y, Dai CC, Wu YC, Yuan ZL (2004) Study on the degradation of wheat straw by endophytic fungi. Acta Sci Circumst 24:144–149Google Scholar
- Su CL, Wang HW, Xie XG, Zhang W, Li XG, Wang XX, Dai CC (2016) Effects of endophytic fungi and Atractylodes lancea powder on rhizosphere microflora and trace elements during continuous peanut cropping. Acta Ecol Sin 36:2052–2065Google Scholar
- Tejada-Jiménez M, Gil-Díez P, León-Mediavilla J, Wen J, Mysore KS, Imperial J, González-Guerrero M (2017) Medicago truncatula Molybdate transporter type 1 (MtMOT1.3) is a plasma membrane molybdenum transporter required for nitrogenase activity in root nodules under molybdenum deficiency. New Phytol 216:1223–1235CrossRefGoogle Scholar