Advertisement

Culturable Stress-Tolerant Plant Growth-Promoting Bacterial Endophytes Associated with Adhatoda vasica

  • Pratibha VyasEmail author
  • Ramanpreet Kaur
Research Article
  • 2 Downloads

Abstract

Endophytes are potential source of various novel compounds that help to promote plant growth, eliminate plant pathogens, and enable the plant to resist stress-like conditions. The present study aimed at selecting stress-tolerant bacterial endophytes with plant growth-promoting ability from Adhatoda vasica. Salt-tolerant bacterial endophytes were isolated on nutrient agar with 2.5% NaCl from the leaves of Adhatoda vasica collected from Manipur, India. The isolates were screened for stress tolerance, plant growth-promoting traits, antagonism against fungal phytopathogens and plant growth promotion. Sixteen morphologically distinct salt-tolerant bacterial endophytes were isolated from Adhatoda vasica. All bacterial endophytes showed auxin production while phosphate solubilization was shown by 81% isolates, siderophore production by 75%, ACC deaminase by 43.8% isolates, HCN by 50% isolates, and ammonia by 62.5% isolates. Four bacterial isolates showed antagonistic activity against all the test fungal phytopathogens Fusarium verticillioides (MTCC 3322), Curvularia lunata (MTCC 283), and Alternaria alternata (MTCC 1362). Dendrogram generated based on stress tolerance of the bacterial isolates against salinity, temperature, pH, and calcium salts showed 3 clusters and two independent branches. Two bacterial isolates identified based on phenotypic features and 16S rRNA gene sequencing as Bacillus thuringiensis A1B3 and Bacillus sp. A1B6 significantly increased the growth parameters of pea and maize in comparison to uninoculated control in pots under natural conditions. The attributes of stress tolerance, antagonism against fungal pathogens, and plant growth promotion indicated the potential of Bacillus thuringiensis A1B3 and Bacillus sp. A1B6 to be used as microbial inoculant in agriculture under stressed environment.

Keywords

Endophytes Adhatoda vasica Antagonism Plant growth promotion Abiotic stress tolerance 

Notes

Acknowledgements

The authors acknowledge the Vice Chancellor, Punjab Agricultural University, Punjab and the Chancellor, Lovely Professional University, Punjab, for providing the necessary facilities.

Funding information

This study was financially supported by the Chancellor, Lovely Professional University, Punjab, to carry out the research studies.

References

  1. Cappuccino JC, Sherman N (1992) Microbiology: a laboratory manual. Benjamin/Cummings Publishing Co., New York, pp 125–179Google Scholar
  2. Castric PA (1975) Hydrogen cyanide, a secondary metabolite of Pseudomonas aeruginosa. Can J Microbiol 21(5):613–618CrossRefGoogle Scholar
  3. Das P, Behera BK, Meena DK, Azmi SA, Chatterjee S, Meena K, Sharma AP (2015) Salt stress tolerant genes in halophilic and halotolerant bacteria: paradigm for salt stress adaptation and osmoprotection. Int J Curr Microbiol App Sci 4(1):642–658Google Scholar
  4. Dodd IC, Pérez-Alfocea F (2012) Microbial amelioration of crop salinity stress. J Exp Bot 63(9):3415–3428CrossRefGoogle Scholar
  5. Ghosh R, Barman S, Khatun J, Mandal NC (2016) Biological control of Alternaria alternata causing leaf spot disease of Aloe vera using two strains of rhizobacteria. Biol Control 97:102–108CrossRefGoogle Scholar
  6. Glick BR (2014) Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol Res 169(1):30–39CrossRefGoogle Scholar
  7. Gulati A, Rahi P, Vyas P (2008) Characterization of phosphate-solubilizing fluorescent pseudomonads from the rhizosphere of seabuckthorn growing in the cold deserts of Himalayas. Curr Microbiol 56(1):73–79CrossRefGoogle Scholar
  8. Gulati A, Vyas P, Rahi P, Kasana RC (2009) Plant growth-promoting and rhizosphere-competent Acinetobacter rhizosphaerae strain BIHB 723 from the cold deserts of the Himalayas. Curr Microbiol 58(4):371–377CrossRefGoogle Scholar
  9. Harris LJ, Daeschel MA, Stiles ME, Klaenhammer TR (1989) Antimicrobial activity of lactic acid bacteria against Listeria monocytogenes. J Food Prot 52:384–387CrossRefGoogle Scholar
  10. Kaur R, Devi MA, Vyas P (2017) Endophytic Pseudomonas sp. TCA1 from Tinospora cordifolia stem with antagonistic and plant growth-promoting potential. Res J Pharmacy Technol 10(2):456–460CrossRefGoogle Scholar
  11. Kaur A, Devi SR, Vyas P (2018) Stress-tolerant antagonistic plant growth-promoting rhizobacteria from Zea mays. J Plant Prot Res 58:115–123Google Scholar
  12. Krieg NR, Holt JG (1984) Bergey’s manual of systematic bacteriology Vol 1. Williams and Willkins, Baltimore, p 964Google Scholar
  13. Kumar M, Dandapat S, Kumar A, Sinha MP (2013) Determination of nutritive value and mineral elements of five-leaf chaste tree (Vitex negundo L.) and Malabar nut (Adhatoda vasica Nees). Atmosphere 7:8Google Scholar
  14. Lal M, Kumar S, Ali M, Khan A, Singh V, Murti S (2013) Host range, susceptibility period of Curvularia lunata causing leaf spot of black gram and germplasm screening. Agriways 1:142–146Google Scholar
  15. Loper JE, Schroth MN (1986) Influence of bacterial sources of indole-3-acetic acid on root elongation of sugar beet. Phytopathol 76(4):386–389CrossRefGoogle Scholar
  16. Matos AD, Gomes IC, Nietsche S, Xavier AA, Gomes WS, Dos Santos Neto JA, Pereira MC (2017) Phosphate solubilization by endophytic bacteria isolated from banana trees. An Acad Bras Cienc 89(4):2945–2954CrossRefGoogle Scholar
  17. Mohamad OA, Li L, Ma JB, Hatab S, Xu L, Guo JW, Rasulov BA, Liu YH, Hedlund BP, Li WJ (2018) Evaluation of the antimicrobial activity of endophytic bacterial populations from Chinese traditional medicinal plant Licorice and characterization of the bioactive secondary metabolites produced by Bacillus atrophaeus against Verticillium dahliae. Front Microbiol 9Google Scholar
  18. Nautiyal CS (1999) An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol Lett 170:265–270CrossRefGoogle Scholar
  19. Paz ICP, Santin RCM, Guimarães AM, Rosa OPP, Dias ACF, Quecine MC, Azevedo JL, Matsumura ATS (2012) Eucalyptus growth promotion by endophytic Bacillus spp. Genet Mol Res 11(4):3711–3720CrossRefGoogle Scholar
  20. Reetha AK, Pavani SL, Mohan S (2014) Hydrogen cyanide production ability by bacterial antagonist and their antibiotics inhibition potential on Macrophomina phaseolina (Tassi.) Goid. Int J Curr Microbiol App Sci 3:172–178Google Scholar
  21. Ribeiro VP, Marriel IE, de Sousa SM, de Paula Lana UG, Mattos BB, de Oliveira CA, Gomes EA (2018) Endophytic Bacillus strains enhance pearl millet growth and nutrient uptake under low-P. Braz J Microbiol 49:40–46CrossRefPubMedCentralGoogle Scholar
  22. Saikia J, Sarma RK, Dhandia R, Yadav A, Bharali R, Gupta VK, Saikia R (2018) Alleviation of drought stress in pulse crops with ACC deaminase producing rhizobacteria isolated from acidic soil of Northeast India. Sci Rep 8:3560CrossRefPubMedCentralGoogle Scholar
  23. Sayyed RZ, Chincholkar SB (2009) Siderophore-producing Alcaligenes feacalis exhibited more biocontrol potential Vis-à-Vis chemical fungicide. Curr Microbiol 58:47–51CrossRefGoogle Scholar
  24. Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56CrossRefGoogle Scholar
  25. Tan D, Fu L, Han B, Sun X, Zheng P, Zhang J (2015) Identification of an endophytic antifungal bacterial strain isolated from the rubber tree and its application in the biological control of banana Fusarium wilt. PLoS One 10:e0131974CrossRefPubMedCentralGoogle Scholar
  26. Tsavkelova EA, Cherdyntseva TA, Botina SG, Netrusov AI (2007) Bacteria associated with orchid roots and microbial production of auxin. Microbiol Res 162:69–76CrossRefGoogle Scholar
  27. Vardharajula S, Zulfikar Ali S, Grover M, Reddy G, Bandi V (2011) Drought-tolerant plant growth promoting Bacillus spp.: effect on growth, osmolytes, and antioxidant status of maize under drought stress. J Plant Interact 6:1–14CrossRefGoogle Scholar
  28. Vyas P, Kaur R (2017) Plant growth-promoting and antagonistic endophytic bacteria from the medicinal plant Tinospora cordifolia stem. Inter J Res Pharm Sci 8:196–199Google Scholar
  29. Vyas P, Kaur A (2018) Stress-tolerant antagonistic rhizobacteria isolated from the medicinal plant Tinospora cordifolia. Biotechnologia 99:129–136CrossRefGoogle Scholar
  30. Vyas P, Rahi P, Gulati A (2009) Stress tolerance and genetic variability of phosphate-solubilizing fluorescent Pseudomonas from the cold deserts of the trans-Himalayas. Microb Ecol 58:425–434CrossRefGoogle Scholar
  31. Vyas P, Joshi R, Sharma KC, Rahi P, Gulati A, Gulati A (2010) Cold-adapted and rhizosphere-competent strain of Rahnella sp. with broad-spectrum plant growth-promotion potential. J Microbiol Biotechnol 2:1724–1734Google Scholar
  32. Zalila-Kolsi I, Mahmoud AB, Ali H, Sellami S, Nasfi Z, Tounsi S, Jamoussi K (2016) Antagonist effects of Bacillus spp. strains against Fusarium graminearum for protection of durum wheat (Triticum turgidum L. subsp. durum). Microbiol Res 192:148–158CrossRefGoogle Scholar

Copyright information

© Sociedad Chilena de la Ciencia del Suelo 2019

Authors and Affiliations

  1. 1.Department of Microbiology, College of Basic Sciences and HumanitiesPunjab Agricultural UniversityLudhianaIndia
  2. 2.Department of Microbiology, School of Bioengineering and BiosciencesLovely Professional UniversityPhagwaraIndia

Personalised recommendations