Nodulation and Genomic Capacity of a Novel High-Latitude Bradyrhizobium japonicum HLNEAU001
Rhizobium species are essential symbionts of legumes, participating in nitrogen fixation and having an important impact on the protein content of soybeans. In this study, we isolated Rhizobia from the soybean cultivar Suinong14 growing in a soybean field in Harbin (45.75° N, 126.53° E), which is situated within one of the three largest black soil belts in the world. One Bradyrhizobium japonicum strain (High-Latitude Northeast Agricultural University 001, HLNEAU001) was identified. Two hundred fifteen core soybean germplasms from Northeast China were screened for nodule traits, with the nodule number ranging from 0 to 95. Phylogenetic analysis showed that B. japonicum HLNEAU001 is closely related to B. japonicum USDA6 and B. diazoefficiens USDA110. A nodulation capacity analysis showed that B. japonicum HLNEAU001 formed more nodules than B. japonicum USDA6 or B. diazoefficiens USDA110, with 30 soybean germplasms being assayed for each strain. Using a draft genome sequence of B. japonicum HLNEAU001 to compare the genomic differences between B. japonicum HLNEAU001, B. japonicum USDA6 and B. diazoefficiens USDA110 showed that the three strains contain 5790 core genes. Because B. japonicum HLNEAU001 and B. japonicum USDA6 exhibited collinearity, the genomic differences between these two strains were further analysed. In addition to type IV and type VI secreted proteins, we hypothesize that type III effectors are the key factors underlying the nodulation capacity differences between B. japonicum HLNEAU001 and B. japonicum USDA6. The results of this study indicate that B. japonicum HLNEAU001 is a distinctive cold-region, slow-growing Rhizobium strain that is capable of effective symbiotic nitrogen fixation in cold regions and black soil and may play a pivotal role in sustainable agricultural production.
KeywordsBlack soil belts Bradyrhizobium japonicum Genome Nodulation Germplasm Plant soil
The promote project for young talents of the colleges in Heilongjiang province (UNPYSCT-2016008). Financial support was received from The Ministry of Science and Technology of People’s Republic of China Project (2017YFE0111000); EUCLEG (727312); National Natural Science Foundation of China (31400074, 31271747, 31471516, 31401465, 31501332); National Key R&D Program of China (2016YFD0100500, 2016YFD0100300, 2016YFD0100201); Natural Science Foundation of Heilongjiang Province of China (Grant number ZD201213); Heilongjiang Postdoctoral Science Foundation (LBH-Q16014); Harbin Science Technology Project (2013RFQXJ005; 2014RFXXJ012) and Academic Backbon’ Project of Northeast Agricultural University (15XG02).
- Cao MM, Qiao LI, Zhang LY, Gao J, Wei-Hai LI, Ding WM, Sun YK (2014) Accumulated temperature variation and accumulated temperature rezone in Heilongjiang Province. Chin J AgrometeorolGoogle Scholar
- Dâmiany PO, Marislaine AF, Bruno LS, Otávio HST, Fábio ADM, Márcia R et al (2017) Acid tolerant Rhizobium strains contribute to increasing the yield and profitability of common bean in tropical soils. J Soil Sci Plant Nutr 17:922–934Google Scholar
- Hongkun Z, Yumin W, Fu X, Xiaodong L, Cuiping Y, Guangxun Q et al (2018) The genetic diversity and geographic differentiation of the wild soybean in Northeast China based on nuclear microsatellite variation. Int J Genomics 2018:1–9Google Scholar
- Hueck CJ (1998) Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev 62:379Google Scholar
- Keyser HH, Griffin RF (1987) Beltsville hizobium culture collection catalog. ArsGoogle Scholar
- Kneen BE, Larue TA (1983) Congo red absorption by Rhizobium leguminosarum. Appl Environ Microbiol 45:340–342Google Scholar
- Pádua Oliveira D, Alves de Figueiredo M, Lima Soares B, Stivanin Teixeira OH, Dias Martins FA, Rufini M, Peixoto Chain C, Pereira Reis R, Ramalho de Morais A, de Souza Moreira FM, Bastos de Andrade MJ (2017) Acid tolerant rhizobium strains contribute to increasing the yield and profitability of common bean in tropical soils. JSSPN 17(4):922–934Google Scholar
- Ritsema T, Wijfjes AHM, Lugtenberg BJJ, Spaink HP (1996) Rhizobium nodulation protein Noda is a host-specific determinant of the transfer of fatty acids in Nod factor biosynthesis. Mol Gen Genet MGG 251(1):44–51Google Scholar
- Saghafi D, Ghorbanpour M, Lajayer AB (2018) Efficiency of Rhizobium strains as plant growth promoting rhizobacteria on morpho-physiological properties of Brassica napus L. under salinity stress. J Soil Sci Plant Nutr 18(1):253–268Google Scholar
- Zhao L, Li MM, Xu CJ, Yang X, Li DM, Zhao X et al (2018) Natural variation in GmGBP1 promoter affects photoperiod control of flowering time and maturity in soybean. Plant J 96:967–977Google Scholar
- Zhu XC, Song FB, Xu HW (2010) Arbuscular mycorrhizae improves low temperature stress in maize via alterations in host water status and photosynthesis. Plant Soil 331:129–131Google Scholar