Irradiation as a phytosanitary treatment for controlling, Bactrocera zonata (Saunders) and its impact on different quality attributes of pomegranate fruits

  • A. GabartyEmail author
  • A. Hammad
  • Amira A. K. H. Negm
  • Samia M. Abd-El Hameed
Original Research Article


The study was conducted in order to assess the impact of gamma radiation on the different development stages of peach fruit fly, Bactrocera zonata, and to evaluate the influence of irradiation treatment on the physical, chemical, sensorial and microbiological qualities of pomegranate fruits. Pomegranate fruits were artificially infected with eggs and the 1st, 2nd and 3rd instar larvae of B. zonata and treated with different irradiation doses in the range of 50–400 Gy. The results indicated that no emerging adults were seen when eggs had been exposed to 100 Gy. The 3rd instar larva appeared to be the most tolerant stage to radiation treatment than the 2nd instar larva. Radiation treatment with 350 Gy resulted in 100% mortality in the 3rd instar larva. When adult emergence was used as criterion for measuring the effective irradiation dose, 150 Gy was required for disinfestation of the 3rd instar larva of B. zonata. Irradiation treatment of pupae either 3-or 7- days olds with 300 Gy prevented adult emergence. The irradiation dose level of 150 Gy used for the large – scale confirmatory tests was applied to 17,000 3rd instar larvae of B. zonata in pomegranate fruits resulting in non F1 adults’ production with confidence level of 81.7%. It could be conclude that the irradiation dose of 150 Gy prevented adult emergence from infested pomegranate fruits without causing significant (p ≤ 0.05) effect on the quality characteristics of the pomegranate fruits.


Gamma irradiation Bactrocera zonata Phytosanitary Pomegranate fruits 



This work was conducted as a part of International Atomic Energy Agency (IAEA) coordination research project (Development of Electron Beam and X-rays Application for Food Irradiation D 61024). The authors acknowledge partial financial support from International Atomic Energy Agency (IAEA) under the Coordination Research Project D 61024 contract No.19247∕ RO.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© African Association of Insect Scientists 2019

Authors and Affiliations

  1. 1.Natural Products Research Department, National Center for Radiation Research and Technology (NCRRT)Atomic Energy AuthorityCairoEgypt
  2. 2.Radiation Microbiology Research Department, National Center for Radiation Research and Technology (NCRRT)Atomic Energy AuthorityCairoEgypt
  3. 3.Horticulture insects Department, Plant Protection Research InstituteAgricultural Research CenterGizaEgypt
  4. 4.Horticulture Research InstituteAgricultural Research CenterGizaEgypt

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