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In silico characterization of SAMdC from Pokkali rice and its overexpression in transgenic tobacco

  • Saswati Laha
  • Diptasree Kumar
  • Dibyendu N. Sengupta
  • Gaurab GangopadhyayEmail author
Research Articles
  • 8 Downloads

Abstract

Polyamines help to mitigate salt stress in plants. SAMdC or S-adenosyl methionine decarboxylase is a crucial enzyme in the polyamine biosynthetic pathway. Overexpressing the samdc gene of Pokkali (a salt-tolerant Indica rice landrace) in the model plant tobacco through Agrobacterium-mediated transformation resulted in tobacco plants capable of withstanding high salinity (200 mM) stress. The transgenic tobacco plants maintained a steady level of the higher polyamines and resisted ionic imbalance under salt stress. Since appropriate binding of the substrate with the enzyme is essential for an enzymatic reaction, we performed molecular docking experiment of SAMdC enzyme of Pokkali rice and tobacco to get an idea about its commonality in the two plants in the backdrop of Japonica rice and Arabidopsis. In silico characterization of SAMdC revealed that the enzyme used the same substrate in Pokkali rice and tobacco, from where the gene is introgressed and where it is overexpressed.

Keywords

S-adenosylmethionine decarboxylase Docking analysis Pokkali rice Polyamines NaCl stress Overexpression Transgenic tobacco 

Notes

References

  1. Arnon DI (1949) Copper enzymes in isolated chloroplasts: polyphenol oxidase in Beta vulgaris. Plant Physiol. 24:1–15CrossRefGoogle Scholar
  2. Basu R, Mitra N, Ghosh B (1988) Salinity results in polyamine accumulation in early rice (Oryza sativa) seedlings. Aust. J. Plant Physiol. 15:777–786Google Scholar
  3. de Agazio M, Grego S, Ciofi-Luzzatto A, Rea E, Zaccaria ML, Federico R (1995) Inhibition of maize primary root elongation by spermidine: effect on cell shape and mitotic index. J. Plant Growth Regul. 14:85–89CrossRefGoogle Scholar
  4. Gangopadhyay G, Das S, Mitra SK, Poddar R, Modak BK, Mukherjee KK (2002) Enhanced rate of multiplication and rooting through the use of coir in aseptic liquid culture media. Plant Cell Tissue Organ Cult. 68:301–310CrossRefGoogle Scholar
  5. Laha S, Sen S, Ghosh B, Sengupta DN (2014) Characterization of plant S-Adenosyl-L-Methionine Decarboxylase and Spermidine Synthase in polyamine deficient mutant strain of E. coli. Int. Res. J. Biological Sci. 3:60–68Google Scholar
  6. Liu JH, Wang W, Wu H, Gong X, Moriguchi T (2015) Polyamines function in stress tolerance: from synthesis to regulation. Front. Plant Sci. 6:827.  https://doi.org/10.3389/fpls.2015.00827 Google Scholar
  7. Liu Z, Liu P, Qi D, Peng X, Liu G (2017) Enhancement of cold and salt tolerance of Arabidopsis by transgenic expression of the S-adenosylmethionine decarboxylase gene from Leymus chinensis. J. Plant Physiol. 211:90–99CrossRefGoogle Scholar
  8. Luo J, Liu M, Zhang C, Zhang P, Chen J, Guo Z, Lu S (2017) Transgenic Centipede grass (Eremochloa ophiuroides [Munro] Hack.) overexpressing S-Adenosylmethionine Decarboxylase (SAMDC) gene for improved cold tolerance through involvement of H2O2 and NO signalling. Front Plant Sci 8:1655Google Scholar
  9. Manni A, Fischer S, Franks M, Washington S, De Arment R, Griffith J, Demers L, Verderame M, Leiby B, Mauger D (2001) S-adenosylmethionine decarboxylase overexpression reduces invasiveness and tumorigenicity in nude mice of MCF-7 breast cancer cells. Int. J. Oncol. 2:317–323Google Scholar
  10. Marco F, Busó E, Carrasco P (2014) Overexpression of SAMDC1 gene in Arabidopsis thaliana increases expression of defence-related genes as well as resistance to Pseudomonas syringae and Hyaloperonospora arabidopsidis. Front. Plant Sci. 5:115.  https://doi.org/10.3389/fpls.2014.00115 CrossRefGoogle Scholar
  11. Minocha R, Majumdar R, Minocha SC (2014) Polyamines and abiotic stress in plants: a complex relationship. Front. Plant Sci. 5:175–180CrossRefGoogle Scholar
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15:473–497CrossRefGoogle Scholar
  13. Persson K, Slund LA, Grahn B, Hanke J, Heby O (1998) Trypanosoma cruzi has not lost its S-adenosylmethionine decarboxylase: characterization of the gene and the encoded enzyme. Biochem. J. 333:527–537CrossRefGoogle Scholar
  14. Sambrook J, Russel DW (2001) Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY, USAGoogle Scholar
  15. Slocum RD (1991) Polyamine biosynthesis in plants. In: Slocum RD, Flores HE (eds) Biochemistry and Physiology of Polyamines in Plants. CRC Press, Boca Raton, FL, pp 23–40Google Scholar
  16. Xie JH, Zapata-Arias FJ, Shen M, Afza R (2000) Salinity tolerant performance and genetic diversity of four rice varieties. Euphytica 116:105–110CrossRefGoogle Scholar
  17. Yang J, Roy A, Zhang Y (2013a) Protein-ligand binding site recognition using complementary binding-specific substructure comparison and sequence profile alignment. Bioinformatics 29:2588–2595CrossRefGoogle Scholar
  18. Yang J, Roy A, Zhang Y (2013b) BioLiP: a semi-manually curated database for biologically relevant ligand-protein interactions. Nucleic Acids Res 41(database issue):D1096–D1103Google Scholar
  19. Yesmin N, Elias SM, Rahman MS, Haque T, Mahbub Hasan AKM, Seraj ZI (2014) Unique genotypic differences discovered among indigenous Bangladeshi rice landraces. Int J Genom. https://doi.org/10.1155/2014/210328 (Article ID 210328)
  20. Zhang Y (2008) I-TASSER server for protein 3D structure prediction. BMC Bioinformatics.  https://doi.org/10.1186/1471-2105-9-40 Google Scholar

Copyright information

© Society for Plant Research 2019

Authors and Affiliations

  • Saswati Laha
    • 1
  • Diptasree Kumar
    • 2
  • Dibyendu N. Sengupta
    • 2
  • Gaurab Gangopadhyay
    • 2
    Email author
  1. 1.Department of BotanyBethune CollegeKolkataIndia
  2. 2.Division of Plant BiologyBose Institute (Main Campus)KolkataIndia

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