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Antagonistic activity of phylloplane yeasts from Moringa oleifera Lam. leaves against Aspergillus flavus UNJCC F-30 from chicken feed

  • Dalia SukmawatiEmail author
  • Marsha Hanin Andrianto
  • Zico Arman
  • Nuniek Ina Ratnaningtyas
  • Shabrina Nida Al Husna
  • Hesham Ali El-Enshasy
  • Daniel Dailin
  • Ahmed Atta Kenawy
Research Article
  • 2 Downloads

Abstract

Aspergillus flavus is widely known as an aflatoxin-producing fungus that frequently contaminates feed and affects livestock, which leads to severe health problem for animal and human. Biological agents have been proven to prevent this contamination since they can produce metabolites which have antagonistic activity. In this study, phylloplane yeasts isolated from Moringa oleifera leaf have shown an ability to inhibit the growth of Aspergillus flavus UNJCC F-30 collected from chicken feed. This research was conducted in three stages: (1) yeast isolation (leaf washing and direct method), followed by (2) antagonistic test using dual culture method, and (3) molecular identification using D1/D2 region of 26S rDNA. In the first stage, 38 yeast isolates have been succesfully obtained. These isolates were of different colors: peach pigment (60.5%), the non-pigmented yeast (26.5%), cream (10%), and orange (3%). Antagonistic activity against A. flavus UNJCC F-30 was tested based on growth, sporulation, and the presence of clear zones. Screening result showed that 12 yeast isolates are capable of inhibiting A. flavus UNJCC F-30. Among them, 4 isolates with the code K4, K10, K15, and K26 showed the highest antagonist ability. Molecular identification resulted that the 4 isolates show a similar identity with Aureobasidium pullulans UWFP 993 (100%), Aureobasidium melanogenum QCC:M017/17 (99%), Aureobasidium melanogenum QCC:M017/17 (100%) and Rhodotorula taiwanensis CBS:11729 (99%), respectively. Isolate K10 exhibited the highest percentage of inhibition activity among all isolates which is potential for application as biocontrol agent against A. flavus. As A. pullulans is a common yeast found on leaf surfaces of many Indonesian flora, therefore it can be considered as safe and alternative to reduce fungal contamination from A. flavus in feed chicken.

Keywords

Antangonistic Asperillus flavus Yeast Morinaga oleifera Aureobasidium 

Notes

Acknowledgements

The present research work was supported by Direktorat Riset dan Pengabdian Masyarakat Direktorat Jenderal Penguatan Riset dan Pengembangan Kementrian Riset, Teknologi dan Pendidikan Tinggi Hibah Penelitian Riset Terapan No: 23/SP2H/DRPM/LPPM UNJ/III/2019, grand on behalf of Dalia Sukmawati (2019–2020). We express deep gratitude and appreciation to the Department Biology Universitas Negeri Jakarta Research Grant for supporting. We also appreciate member of Microbiology Lab. for all expertise assistance and technical contributions on the research projects, and also Universitas Negeri Jakarta Culture Collection (UNJCC) for the use of the facilities and availability of isolates. We also acknowledge the support of MOHE and UTM-RMC through HICOE Grant No. R.J130000.7846.4J262. We hope this research could be a starting point for every institution to do further study and findings to give more contribution to the research related area.

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Copyright information

© Indian Phytopathological Society 2020

Authors and Affiliations

  • Dalia Sukmawati
    • 1
    • 2
    Email author
  • Marsha Hanin Andrianto
    • 1
  • Zico Arman
    • 1
  • Nuniek Ina Ratnaningtyas
    • 3
  • Shabrina Nida Al Husna
    • 4
  • Hesham Ali El-Enshasy
    • 5
    • 6
  • Daniel Dailin
    • 5
  • Ahmed Atta Kenawy
    • 6
  1. 1.Department of Biology, Faculty of Mathematics and Natural SciencesUniversitas Negeri JakartaRawamangun, Jakarta TimurIndonesia
  2. 2.Univeritas Negeri Jakarta Culture Collection (UNJCC), Faculty of Mathematics and Natural SciencesUniversitas Negeri JakartaRawamangunIndonesia
  3. 3.Faculty of BiologyUniversitas Jenderal SoedirmanPurwokerto, Jawa TenggahIndonesia
  4. 4.Department of Microbiology, School of Life Sciences and TechnologyInstitut Teknologi BandungBandungIndonesia
  5. 5.Institute of Bioproduct DevelopmentUniversiti Teknologi Malaysia (UTM)Skudai, Johor BahruMalaysia
  6. 6.City of Scientific Research and Technology ApplicationsNew Burg Al Arab, AlexandriaEgypt

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