Bacterial leaf spot on Convolvulus arvensis caused by Pseudomonas sp.

  • Esmaeil Basavand
  • Pejman KhodayganEmail author
  • Heshmatollah Rahimian
  • Aboalfazl Solhizadeh
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Field bindweed (Convolvulus arvensis) is considered as one of the most destructive weeds in the fields and gardens worldwide. In summer 2017, during the investigation carried out in Mazandaran province, a significant number of field bindweed indicated the leaf spot symptoms. Hence, the samples were collected and brought to the phytobacteriology lab for further examination. Then, isolation, purification and biochemical characterization of fifteen isolates were performed based on the standard bacteriological methods. The pathogenicity test of selected isolates on field bindweed plant were evaluated as positive. In order to validate the results, partial of 16S rRNA region were sequenced and phylogenetic trees constructed as well. According to the phenotypic and molecular characteristics, field bindweed isolates were identified as Pseudomonas sp. To the best of the authors’ knowledge, the present study is the first report of bacterial leaf spot on Convolvulus arvensis caused by Pseudomonas sp. in Iran.


Convolvulus arvensis Bacterial leaf spot Pseudomonas sp. 



We thank the Research Deputy of Vali-e-Asr University of Rafsanjan, Iran for providing the financial support.


  1. Ausuble F, Brent FM, Kingestone RE, Moor DD, Smith JA, SeIDeman JG, Struhl K (1992) Current protocol in molecular biology. Wiley Interscience, New YorkGoogle Scholar
  2. Borkar SG (2017) Laboratory techniques in plant bacteriology. CRC Press, New YorkCrossRefGoogle Scholar
  3. Mulet M, Bennasar A, Lalucat J, García-Valdés E (2010) An rpoD-based PCR procedure for the identification of Pseudomonas species and for their detection in environmental samples. Mol Cell Probes 23:140–147. CrossRefGoogle Scholar
  4. Rashed Mohasel M (1998) Field bindweed, the collection and identification of important Iranian weeds. Ferdowsi University of Mashhad Publications, MashhadGoogle Scholar
  5. Schaad NW, Jones JB, Chun W (2001) Laboratory guide for identification of plant pathogenic bacteria. APS Press, MinnesotaGoogle Scholar
  6. Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173(2):697–703. CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Indian Phytopathological Society 2019

Authors and Affiliations

  1. 1.Department of Plant PathologyVali-e-Asr University of RafsanjanRafsanjanIran
  2. 2.Department of Plant PathologySari Agricultural Sciences and Natural Resources UniversitySariIran

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