Biological and molecular evidence of papaya ring spot virus pathotype P from mid-hills of Meghalaya, India
Papaya ring spot virus (PRSV) is the limiting factor of papaya production in India irrespective of agro-climatic conditions and it may cause crop losses up to 85–90%. In this study, diagnostic survey on PRSV was conducted in mid-hills of Ri-Bhoi district, Meghalaya, India during April to June, 2015. Reverse transcription polymerase chain reaction assay detected PRSV infection in 38 of 53 samples collected from surveyed locations with an average incidence of 71.7%. The representative isolate (PRSV-P:Umiam) from mid-hill Meghalaya was maintained in healthy papaya seedlings by standard sap inoculation method and typical symptoms were expressed within 21–24 days post inoculation. Studies on the physical properties revealed that the dilution end point of PRSV-P:Umiam at 10−2, longevity in vitro for 18 h and thermal inactivation point in between 50 and 55 °C. Among the cucurbitaceous hosts, pumpkin (Cucurbita pepo) alone exhibited chlorotic lesions on leaves within 20–25 days post inoculation. The partial coat protein (CP 750 bp) of PRSV-P:Umiam gene shared maximum nucleotide (~ 95%) and amino acid (~ 98%) identity with PRSV isolates reported from northern and eastern India. The CP based phylogeny clustered PRSV-P:Umiam with the PRSV isolates from north and east India.
KeywordsPapaya ring spot virus Characterization Physical property Host range Coat protein
This work is carried out under M.Sc. thesis programme by Korla Sarath Babu. The authors are thankful to Drs. G.T. Behere, Sandip Patra and T. Rajesh for their critical suggestions during the work. The work was supported by Indian Council of Agricultural Research.
- Bateson MF, Lines RE, Reville P, Chaleeprom W, Ha CV, Gibbs AJ, Dale JL (2002) On the evolution and molecular epidemiology of the potyvirus papaya ringspot virus. J Gen Biol 83:2575–2585Google Scholar
- Capoor SP, Varma PM (1958) A mosaic disease of papaya in Bombay. Indian J Agric Sci 28:225–235Google Scholar
- Jensen DD (1949) Papaya virus diseases with special reference to papaya ringspot. Phytopathology 39:191–211Google Scholar
- King AM, Adams MJ, Lefkowitz EJ, Carstens EB, Ball LA (2011) Virus taxonomy: IXth report of the international committee on taxonomy of viruses. Elsevier Academic Press, LondonGoogle Scholar
- Kunkalikar S, Byadgi AS (2004) Molecular characterization, cloning of coat protein gene, epidemiology and management of papaya ringspot virus. Indian Phytopath 57:330–331Google Scholar
- Purcifull DE, Edwardson JR, Hiebert E, Gonsalves D (1984) Papaya ringspot virus. CMI/AAB descriptions of plant virus, no 292. CAB International, WallingfordGoogle Scholar
- Sharma J, Jain RK, Varma A (2004) Detection of Papaya ring spot virus in naturally infected papaya plants by reverse transcription polymerase chain reaction. Indian Phytopath 57(2):237–239Google Scholar
- Singh RK, Singh D, Singh SJ (2003) Incidence, distribution and detection of a virus infecting papaya (Carica papaya L.) in eastern Uttar Pradesh. Indian J Plant Pathol 21(1-2):51–56Google Scholar
- Wang HL, Wang CC, Chiu RJ, Sun MH (1978) Preliminary study on papaya ringspot virus in Taiwan. Plant Prot Bull 20:133–140Google Scholar