DNA-A sequences of whitefly transmitted begomovirus infecting okra (Abelmoschus esculentus) in India are extensively diverse
- 9 Downloads
Okra or bhendi (Abelmoschus esculentus) is one of the most popular and widely grown vegetable crops in India. Whitefly (Bemisia tabaci) transmitted begomoviruses cause heavy economic loss in the okra cultivation in India. In the present study, full length annotated DNA-A (~ 2.7 kb) sequences of 134 begomoviruses infecting okra in India available in NCBI database were retrieved, analyzed and compared following with other begomovirus sequences. The sequence analysis reveals extensive genetic diversity among the begomoviruses infecting okra in India showing 69–100% nt identity among them. The present phylogenetic analysis displays an extensive genetic diversity among begomoviruses infecting okra in India showing occurrence of at least 14 Types begomoviruses. Majority of the present sequences fell into two major Types; 45 sequences into Type-5 i.e., Bhendi yellow vein mosaic virus (BYVMV-[IN-Mah-NOL751]EU589392) strain and 44 sequences into Type-9 i.e., BYVMV-Har[IN-Har-07]FN645923 and/or BYVMV-IN[IN-Mad]AF241479 strain. Five Indian sequences fell into Type-7 i.e., BYVMV-TN[IN-Coi4-04]FJ179372 and/or BYVMV-[PK-Fai201-95]AJ002451 strain and eight sequences into Type 1 i.e., Okra enation leaf curl virus (OELCuV,GU111996). Four sequences in Type-13 i.e., Tomato leaf curl New Delhi (ToLCNDV), three in Type-6 i.e., Cotton leaf curl Alabad (CLCuAlV), two in Type 14 i.e., Bhendi yellow vein mosaic Bhubaneswar (BYVBhV) and three in Type-2 i.e., Mesta yellow vein mosaic virus (MeYVMV). The other sequences were distributed into another six distinct types or putative species. Recombination analysis showed that most of the present begomovirus types identified were recombinants. The present study reports that DNA-A sequences of begomovirus infecting okra in India are extensively diverse and originated from recombination events involving diverse begomovirus sequences.
KeywordsBegomovirus Okra/bhendi Genetic diversity Phylogenetic analysis Recombination
The authors are thankful to R. Aggarwal, Head; V. K. Baranwal, In-charge, Plant Virology Unit; Division of Plant Pathology; Director, ICAR-IARI, New Delhi for providing the facility.
- Anonymous (2017) Horticultural Statistics at a Glance 2017. Horticulture Statistics Division, Department of Agriculture, Cooperation & Farmers Welfare, Ministry of Agriculture & Farmers welfare, Government of India. https://www.agricoop.nic.in
- Brown JK (2007) The Bemisia tabaci complex: genetic and phenotypic variability drives begomovirus spread and virus diversification. https://doi.org/10.1094/APSnetFeature/2007-0107
- Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JCF, Fiallo-Olive E, Briddon RW, Hernandez-Zepeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160:1593–1619CrossRefPubMedGoogle Scholar
- Gill RJ, Brown JK (2010) Systematics of bemisia and bemisia relatives: can molecular techniques solve the bemisia tabaci complex conundrum-a taxonomist's viewpoint. In: Stansly P, Naranjo S (eds) Bemisia: bionomics and management of a global pest. Springer, Netherlands, pp 5–29Google Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- Kulkarni CS (1924) Mosaic and other related diseases of crops in the Bombay Presidency. Poona Agriculture College Magazine, Pune, p 16Google Scholar
- Singh SJ (1996) Assessment of losses in okra due to enation leaf curl virus. Indian J Virol 12:51–53Google Scholar