First report of association of begomovirus in yellow mosaic disease of bur cucumber in India

  • Shivani Khanna
  • Sonali Rana
  • Jitender SinghEmail author
  • Manisha Goyal
  • Pankaj Kumar
  • Neetu Singh
  • R. P. Pant
  • V. K. Baranwal
New Reports


In November- December, 2016–2017 Bur cucumber (Sicyos angulatus) plants samples showing severe symptom of yellowing, mosaic and leaf curling were collected from Horticulture research centre (HRC), Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut. Polymerase chain reaction using coat protein gene specific primer (ToLCVAV1F and ToLCVAV1R) gave an amplification of ~ 750 bp, similar to leaf curl virus reported on different crops and weeds. The gene was sequenced and submitted to GenBank with MF471344. BLAST analysis showed the association of Begomovirus with 99% nucleotide sequence identity with Tomato leaf curl Palampur virus (ToLCPalV) strain India: UP: Matera: Tomato [KF157552]. Phylogenetic analysis also supported the relationship of the obtained sequence with other begomoviruses as they clustered in a separate monophyletic cluster with ToLCPalV-Karnataka, India (KY564204, KY564205), India; ToLCPalV-Varanasi, India (FJ931537, GQ225738); ToLCPalV-Matera (KF157552), India and ToLCPalV-Allipur, India (HQ848383). Sequence Identity matrix with other begomovirus sequences was found to be in range between (78 and 99%). To our knowledge, this is the first confirmed evidence of a begomovirus infecting weed bur cucumber (Sicyos angulatus) in India.


Tomato leaf curl Palampur virus Tomato leaf curl New Delhi virus Coat protein Phylogenetic analysis 



The authors acknowledge the Vice Chancellor, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250110, Uttar Pradesh and Bioinformatics facility, Department of Biotechnology, Government of India, for providing financial support and the facilities to carry out this research work.


  1. Ali I, Malik AH, Mansoor S (2010) First report of tomato leaf curl Palampur virus on bitter gourd in Pakistan. Plant Dis 94:276CrossRefGoogle Scholar
  2. Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG (2002) Universal primers for the PCR-mediated amplification of DNA β. Mol Biotechnol 20:315–318CrossRefGoogle Scholar
  3. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus. 12:13–15Google Scholar
  4. Heydarnejad J, Hesari M, Massumi H, Varsani A (2013) Incidence and natural hosts of tomato leaf curl Palampur virus in Iran. Aust Plant Pathol 42:195–203CrossRefGoogle Scholar
  5. Kumar Y, Hallan V, Zaidi AA (2008) Molecular characterization of a distinct bipartite begomovirus species infecting tomato in India. Virus Genes 37:425–431CrossRefGoogle Scholar
  6. Malik AH, Briddon RW, Mansoor S (2011) Infectious clones of tomato leaf curl Palampur virus with a defective DNA B and their pseudo-recombination with tomato leaf curl New Delhi virus. Virol J. 8:173CrossRefGoogle Scholar
  7. Namrata J, Saritha RK, Datta D, Singh M, Dubey RS, Rai AB, Rai M (2012) Molecular characterization of tomato leaf curl Palampur virus and pepper leaf curl betasatellite naturally infecting pumpkin (Cucurbita moschata) in India. Indian J Virol. 21:128–132CrossRefGoogle Scholar
  8. Raj S, Kumar S, Srivastava A (2015) Association of tomato leaf curl Palampur virus with yellow mosaic disease of Armenian cucumber (Cucumis melo var. flexuoses) and wild melon (C. callosus var. agrestis) in India. Arch Phytopathol Pflanzenschutz 48:751–759CrossRefGoogle Scholar
  9. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  10. Singh J, Singh A, Kumar P, Rani A, Baranwal VK, Sirohi A (2013) Evidence of association of a monopartite tomato leaf curl New Delhi virus with chilli leaf curl disease in western Uttar Pradesh, India. Vegetos 26(2):203–211Google Scholar
  11. Tahir M, Haider MS (2006) First report of a begomovirus associated with leaf curl disease of bell pepper in Pakistan. Plant Pathol 55:570CrossRefGoogle Scholar
  12. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefGoogle Scholar

Copyright information

© Indian Phytopathological Society 2019

Authors and Affiliations

  1. 1.College of BiotechnologySardar Vallabhbhai Patel University of Agriculture and TechnologyMeerutIndia
  2. 2.Department of BiotechnologyMewar Institute of ManagementGhaziabadIndia
  3. 3.Division of Plant Pathology, Advance Center for Plant VirologyICAR-Indian Agriculture Research InstituteNew DelhiIndia

Personalised recommendations