Bio-Design and Manufacturing

, Volume 1, Issue 3, pp 171–181 | Cite as

Current advances in solid free-form techniques for osteochondral tissue engineering

  • João Bebiano Costa
  • Joana Silva-Correia
  • Rui Luís Reis
  • Joaquim Miguel Oliveira


Osteochondral (OC) lesions are characterized by defects in two different zones, the cartilage region and subchondral bone region. These lesions are frequently associated with mechanical instability, as well as osteoarthritic degenerative changes in the knee. The lack of spontaneous healing and the drawbacks of the current treatments have increased the attention from the scientific community to this issue. Different tissue engineering approaches have been attempted using different polymers and different scaffolds’ processing. However, the current conventional techniques do not allow the full control over scaffold fabrication, and in this type of approaches, the tuning ability is the key to success in tissue regeneration. In this sense, the researchers have placed their efforts in the development of solid free-form (SFF) techniques. These techniques allow tuning different properties at the micro–macro scale, creating scaffolds with appropriate features for OC tissue engineering. In this review, it is discussed the current SFF techniques used in OC tissue engineering and presented their promising results and current challenges.


Solid free-form Osteochondral Tissue engineering Scaffolds 


Osteochondral (OC) tissue engineering requires unique scaffolds with specific properties, which ideally promote individual growth of both cartilage and bone layers [1]. The OC defects are characterized by an injury in the cartilaginous region, as well as in the underlying subchondral bone, and are frequently related with mechanical instability of the joint. The lack of spontaneous healing and the associated osteoarthritic degenerative changes are leading to an increase awareness from the orthopedic field [2]. Therefore, in an ideal situation, OC repair strategies should: (1) comprise a substitute that is easy and quick to implant; (2) reduce surgical morbidity; (3) not require harvesting of other tissues (e.g., periosteum); and (4) allow an efficient and complete integration of the implant [2]. However, a complex structure that comprises a cartilage–bone interface requires a tissue engineering approach where implants are able to mimic the chondrogenic and osteogenic environment simultaneously. In other words, there must be a compromise between the temporary mechanical function provided and the architectural properties (i.e., pore shape, size and interconnectivity) in order to pursue a better biological environment and tissue regeneration [3]. A paradigm shift is taking place in the field of orthopedic surgery, with the introduction of the use of synthetic or natural implants [4]. Despite being weaker and softer materials, natural polymers have the advantage of being flexible, thus presenting the capability to adapt their shape to the required forms. In addition, natural materials usually contain specific molecular domains that can support and guide cells, enhancing the biological interaction between the scaffold and the host tissue [1]. As example, Oliveira et al. [5] developed a hydroxyapatite/chitosan (HA/CS) bilayered scaffold by combining a sintering with a freeze-drying technique. Two distinct layers were obtained, a porous HA layer and a CS layer corresponding to bone and cartilage zones, respectively. The scaffolds were shown to present adequate porosities and mechanical properties. It was also shown that both layers provided support for cell attachment, proliferation and differentiation into osteoblasts and chondrocytes, respectively. Moreover, since collagen is the major component in the extra-cellular matrix, collagen-based scaffolds have been shown promising results in OC tissue engineering approaches. Levingstone et al. [6] fabricated a collagen layered structure using a novel “iterative layering” freeze-drying technique that allowed to control material composition, pore size and substrate stiffness in each region of the construct. In the end, the authors obtained a gradient structure composed by a bone layer made of type I collagen and HA, an intermediate layer made of type I collagen, type II collagen and HA and a cartilaginous region made of type I collagen, type II collagen and hyaluronic acid. The scaffolds revealed an optimized environment for cell attachment and proliferation. In another study, Zhou et al. [7] developed also a collagen-based layered scaffold composed by a collagen and a collagen/HA part to mimic the cartilage and bone regions, respectively. Human mesenchymal stem cells were used to promote chondrogenic and osteogenic differentiation. The results showed that the collagen layer was more efficient at inducing chondrogenic differentiation, while the collagen/HA layer was superior in the promotion of osteogenic differentiation.

Unlike natural polymers, the synthetic polymers offer a wide range of chemistry and processing options and their production can be scaled up to industrial-scale manufacturing processing, which is a requirement for future clinical applications [1]. However, in general, they have limitations in terms of biocompatibility and bioactivity. As well as in natural polymers, synthetic polymers have been used in combination with HA and ceramics. Huang et al. [8] developed a novel amorphous calcium phosphate (ACP)/poly(L-lactic acid) (PLLA) material incorporating basic fibroblast growth factor (b-FGF) that showed good cartilage integration after 12 weeks implantation in a rabbit model. In another study, Jiang et al. [9] implanted a biphasic poly (DL-lactic-co-glycolide)/calcium phosphate construct into mini-pigs for 6 months. Despite the poor integration with the surrounding cartilage, histology revealed good bone integration and a tidemark was noted between cartilage and bone.

Nevertheless, although conventional techniques (i.e., solvent casting, phase separation, electro-spinning, salt-leaching, freeze-drying) have some capacity to tune the scaffolds pore size and porosity, they will never be able to completely control the morphology and architecture of scaffolds in terms of pore size, geometry, interconnectivity and spatial distribution. As alternative, several researchers have recently changed their attention to solid free-form (SFF) technologies. Commonly known as SFF techniques, rapid prototyping (RP) or additive manufacturing (AM), rely on the use of computer-aided design (CAD) to build structures by selectively adding materials layer-by-layer [10]. Furthermore, medical scans, such as computerized tomography (CT) or magnetic resonance imaging (MRI), can be used to create a personalized CAD model to produce patient-specific implants [11]. In other words, SFF techniques can be a huge help in OC tissue engineering, because it allows the fine tuning of different materials’ properties at the micro- and macro-levels, creating scaffolds with specific mechanical properties and with an appropriate biological environment for bone and chondral tissue differentiation [12]. For these reasons, and combining all these advantages with the high degree of reproducibility and homogeneity, SFF technique is considered the current “golden strategy” for the generation of scaffolds presenting significant benefits over conventional porous scaffold production technologies [13].

The schematics of solid free-form (SFF) techniques used in osteochondral (OC) tissue engineering are depicted in Fig. 1.
Fig. 1

Schematic diagram of solid free-form (SFF) techniques used in osteochondral (OC) tissue engineering

The overview of the significant reports on SFF technologies in OC tissue engineering approaches using different methods for scaffolding fabrication is presented herein.

SFF techniques used in OC scaffold fabrication


Considered a pioneer technique in SSF, stereolithography (SL) is a laser-based approach that follows basic principles. An ultraviolet (UV) laser irradiates the top of a bath composed by a photopolymerizable liquid polymer material. As polymerization starts, the laser creates a solid layer by tracing the laser beam along the model boundaries and internal structure leading to the formation of a cross-sectional structure (layer). This polymerization process is repeated, creating overlapped layers that, after successive stacking, lead to the formation of the 3D construct. In the end, the platform is raised, and the excess of resin is drained. The resolution of this technique is not impressive (80–250 mm) and is dependent on the elevator layer resolution and laser spot size [14]. To overcome SL low-resolution values, micro-stereolithography (MSL) was developed to provide higher precision. This technique, based on the same principles of SL, has the capability to offer resolutions around 1–2 µm due to the presence of a focusing lens.

The performance of both techniques is highly dependent on the photo-polymerization reaction. In this sense, there is a limited choice in terms of biomaterials with good photo-polymerization capacity and with the adequate properties (e.g., biocompatibility, biodegradability and mechanical properties) for tissue engineering applications. Many researchers opted to synthesize or modify existent polymers such as polypropylene fumarate, polyethylene glycol (PEG), polyvinyl alcohol (PVA) or polycaprolactone (PCL), in order to create biodegradable polymers [15]. Recently, Bian et al. [16] designed β-tricalcium phosphate (TCP)/collagen scaffolds for OC tissue engineering. In this work, the authors used SL to build a ceramic scaffold, where they subsequently added the cartilage zone (collagen) by gel casting. The final osteochondral scaffold presented fully interconnected pores (700–900 μm) and supported cell adhesion and proliferation up to 7 days of culturing. Later, Bian et al. [17] used histology, micro-computed tomography (micro-CT) and scanning electron microscopy (SEM) to investigate the microstructure of the cartilage–bone transitional structures in order to improve the biomimetic design of the OC scaffold. A new CAD model was developed, allowing the discovery that the subchondral bone plate is not an intact plate and the presence of some scattered defects allows the blood vessel invasion and nutritional supply.

In another study, Zhang et al. [18] fabricated PEG/β-TCP OC scaffolds using, as in previous work, SL and gel casting. However, unlike the previous approach, the authors produced a β-TCP ceramic scaffold using the gel casting process, while for the chondral zone it was used SL. The PEG hydrogel was directly cured on the ceramic scaffolds giving origin to a bilayer OC scaffold. The scaffolds were implanted in rabbit trochlea model within a critical size defect. The animals were euthanized at 1, 2, 4, 8, 16, 24 and 52 weeks after implantation. This work revealed good outcomes and the authors concluded that subchondral bone migration is related with cartilage regeneration in critical size osteochondral defects. In a different and more advanced approach, Castro et al. [19] developed two biologically inspired nanomaterials: (1) osteoconductive nanocrystalline hydroxyapatite (nHA) (primary inorganic component of bone) and (2) core–shell poly(lactic-co-glycolic) acid (PLGA) nanospheres encapsulated with the transforming growth-factor β1 (TGF-β1). The authors used a novel table-top SL 3D printer to fabricate a hierarchical scaffold with the aim to provide biological cues at nano- and microscales (Fig. 2a). In the end, the scaffolds were able to mimic the native tissue supporting cell adhesion, proliferation and osteochondral differentiation (Fig. 2b).
Fig. 2

a (i–iii) 3D CAD model (bottom, top and side view) of the three-layer osteochondral scaffold design with 60% in-fill density. SEM images of (iv–vi) control scaffolds without nHA (bottom and top images); and (vii–ix) osteochondral scaffolds with graded nHA (vi is the bottom, vii is the top, viii is 10% nHA layer, and ix is 20% nHA layer). b Three- (i,ii,iii,iv) and five-day (v,vi) hMSC spreading morphology on 3D printed scaffolds containing spatially distributed nHA (graded) when compared to controls. After 3 days of culture, hMSCs display excellent spreading when compared to the spherical morphology of hMSCs seeded upon control scaffolds. In addition, increased cell growth density is observed through DAPI staining of cell nuclei. Scale bars: a 100 µm (iv–viii), 2 µm (vi, ix). b 2 mm (i, ii), 100 µm (iii–vi).

Reprinted with permission from Ref. [19]

However, the limited number of resins available for stereolithography applications is one of the main drawbacks in this type of approach. Ronca et al. [20] developed an acrylic photo-cross-linkable resin based on methyl methacrylate (MMA), butyl methacrylate (BMA) and poly(ethylene glycol) dimethacrylate (PEGDA) with different compositions. The resins were further characterized in terms of mechanical, thermal and biological behavior. The cross-linked materials revealed good mechanical properties and thermal stabilities; moreover, cytotoxicity tests confirmed their biocompatibility with no cytotoxic effect on cells metabolism. In addition, two different treatments have been proposed, using fetal bovine serum (FBS) and methanol (MeOH). The results showed that the samples treated with MeOH allowed cell adhesion and survival, promoting spreading, elongation and fusion of the cells.

Stereolithography, as a SFF technique, enables the production of personalized OC scaffolds controlling the morphology and architecture of the structures. Despite the promising outcomes obtained through in vitro analysis and in vivo animal studies, very few scaffolds fabricated by SL have been evaluated in clinical trials.

Using the same principles, other laser-assisted techniques have been attempted in the OC field. Du et al. [21] have recently developed a new approach to produce bio-inspired multilayer osteochondral scaffold made of poly(ε-caprolactone) (PCL) and hydroxyapatite (HA)/PCL microspheres, through a selective laser sintering (SLS) technique. The results showed that the scaffolds revealed excellent in vitro biocompatibility, as well as great in vivo performance by inducing articular cartilage formation and subchondral bone regeneration in a rabbit model. In this sense, SLS can be a good alternative not only for OC tissue engineering, but also for the fabrication of bio-inspired multilayer scaffolds with well-designed architecture and gradient composition. Furthermore, a recent study performed by Fousová et al. [22] showed the comparison of other two laser-assisted techniques in the scaffold’s production. In this work, the authors compared the architecture and mechanical performance of solid free-form scaffolds composed by a Ti6Al4 V alloy. The Ti6Al4 V alloy is one of the most commonly used implants in orthopedic surgery and already showed promising results in terms of in vitro and in vivo performance [23]. The scaffolds were produced by selective laser melting (SLM) and electron beam melting (EBM). Interestingly, although the results have revealed some similarities in terms of microstructure, due to differences in surface roughness and specific internal defects, the fatigue strength of the EBM samples reached only half the value of the SLM samples. In short, this showed that the use of different solid free-form approaches could lead to different behaviors in terms of mechanical properties and architecture.

Fused deposition modeling

The fused deposition modeling (FDM) is one of the most famous and traditional SFF techniques. Briefly, a head-heated liquefier cartridge melts the filament and pushing it through a nozzle directly on the build platform. The melted thin filament is guided using a carriage that moves in the horizontal x,y plane and builds a layer-by-layer 3D construct. Once a layer is assembled, the build platform moves down in the z direction in a distance correspondent to the layer thickness and starts to deposit the next layer. This method does not require the use of harsh solvents; however, the polymers used in FDM techniques are restricted to thermoplastic materials, disabling cell encapsulation into the constructs during the fabrication process [10].

PCL is a thermoplastic that has been constantly used in OC tissue engineering. However, although PCL can generate mechanically stable constructs, the lack of osteoconductive factors, such as TCP or HA, has led to its combination with ceramics. In 2003, Endres et al. [24] assessed the osteogenic potential of human adipose stem cells in PCL/HA constructs. In that work, the authors reported encouraging results showing cell proliferation toward and onto the PCL/HA scaffolds surfaces. Heo et al. [25] developed nano- and micro-sized HA/PCL composite three-dimensional scaffolds with potential for bone tissue engineering applications. These potential was proved later in an in vivo study, since 8 weeks after implantation in a rabbit tibial segmental defect model, dense bone formation was observed throughout the constructs [26]. Swieszkowski et al. [27] developed a biphasic OC scaffold by FDM. The scaffolds were composed of a PCL–TCP phase for the bone region, and a PCL–Fibrin phase for the cartilage region. Bone marrow-derived mesenchymal cells were isolated and seeded into the scaffolds that were subsequently implanted in medial condyle critical size defects of the rabbit model. Micro-CT analysis revealed significant regeneration in the bone phase. Moreover, the fast degradability of the fibrin restrained the cartilage healing in the PCL–Fibrin region. More recently, Ding et al. [28] developed also a biphasic scaffold, which comprised a cartilage region made of polylactic acid-coated polyglycolic acid (PGA/PLA) and a bone region made of PCL/HA. As usual in SFF approaches, CAD technology helped the authors to produce a scaffold suitable for the regeneration of goat femoral head. Chondrocytes and bone marrow stromal cells (BMSCs) were seeded into the scaffolds for cartilage and bone regeneration, respectively, and subsequently implanted subcutaneously in nude mice. After 10 weeks, the regenerated femoral heads presented smooth, continuous and homogeneous articular cartilage layer and a good subchondral bone integration. Recently, Holmes et al. [29] created novel osteochondral scaffolds with both excellent interfacial mechanical properties and biocompatibility for facilitating human bone marrow mesenchymal stem cell (MSC) growth and chondrogenic differentiation. In this sense, the authors designed and printed a series of innovative biphasic 3D models that mimic the osteochondral region of articulate joints. The mechanical testing results showed suitable mechanical properties under compression (a maximum Young’s modulus of 31 MPa) and shear (a maximum fracture strength of 5768 N/mm2) when compared with homogenous designs. In addition, in order to improve their biocompatibility, the authors modified the surface of the scaffolds with acetylated collagen. The biological assays revealed that the surface modification enhanced MSC proliferation up to 5 days of in vitro culturing. A 2-week chondrogenic differentiation was also performed with the cells presenting good indication of chondrogenic differentiation. Santis et al. [30] used two solid free-form technologies to fabricate PCL- and PEG-based magnetic nanocomposite scaffolds. These scaffolds were fabricated using fused deposition modeling and stereolithography approaches in order to produce a hybrid scaffold. The viscoelastic properties under compression were investigated at 37 °C, spanning a range frequency of four decades. The results suggested that hybrid scaffolds adequately reproduce viscoelastic properties of subchondral bone and articular cartilage tissues, respectively. By means of combining FDM and SL, it was possible to produce a hybrid scaffold suitable for osteochondral tissue regeneration.

Using different polymers, Woodfield et al. [31] fabricated porous scaffolds from a poly(ethylene glycol)-terephthalate poly(butylene terephthalate) (PEGT/PBT) block copolymer. The influence of different PEGT/PBT compositions and pore geometries in the scaffolds’ mechanical behavior was tested, as well as the scaffolds capability to support cell adhesion and proliferation. The scaffolds revealed good biological performance showing its ability to support cell proliferation and matrix synthesis. Later, the same group also revealed better biological results when compared FDM produced scaffolds with more traditional particulate leached scaffolds [32]. The author’s hypothesized that the superior nutrient and oxygen diffusion caused by the orientated pores of the FDM scaffold improved cell viability in the central region of the scaffolds. The effectiveness of porous polyethylene-oxide-terephthalate/polybutylene-terephthalate (PEOT/PBT) scaffolds (Fig. 3a) seeded with MSC was also evaluated in an osteochondral defect using a rabbit model [33]. Regarding chondrogenesis, the results showed evidence of GAG accumulation in the empty defect and around the scaffolds struts of the cell-free scaffold (Fig. 3b–d). Chondrocyte cells were observed in their lacunae above the tidemark in the cell-seeded scaffolds (Fig. 3dii–iii). There was evidence of hypocellularity in the cell-free scaffolds (Fig. 3ciii–iv). In addition, chondrocyte clusters were observed in the cell-seeded constructs (Fig. 3div) and the adjacent cartilage at the margin of the defect in the empty defects (Fig. 3biii–iv). Succinctly, the FDM scaffolds provided both biological cues and mechanical support and enabled to obtain enhanced hyaline-like tissue repair.
Fig. 3

a SEM images showing the 3D scaffold architecture from (i) top view and (ii) cross section. Representative images showing toluidine blue staining for chondrogenesis and GAG accumulation in b an empty defect; c a cell-free PEOT/PBT scaffold, and d a rabbit MSC-seeded scaffold, with insets taken at higher magnifications of ×4 and ×10 to show tissue repair at the edge and the center of the defects as highlighted by dotted black boxes. Dotted red box shows original defect site areas.

Reprinted with permission from Ref. [33]

Recently, the same group developed advanced strategies to better mimic the native tissue. Different pore size gradients revealed stimulation of different cell behaviors. In this sense, the authors showed the capability to tune the scaffolds’ architecture using FDM to achieve an improved induction of mesenchymal stem cells chondrogenesis and osteogenesis [34, 35].

Although FDM technique already showed promising results, there is still the need to see if the in vitro improvements will be translated into enhanced OC regeneration in vivo.

3D bioprinting

In the previous two SFF techniques, the control of cell and growth factors distribution inside the scaffold was not possible, which limits the provision of an ideal microenvironment for cell migration and differentiation. In this sense, new strategies have been developed, namely approaches based on the 3D bioprinting. This SFF technique differs from the others due to the capability to produce cell-leaden scaffolds with specific cell types, cell densities or with a specific growth factor. In other words, besides the possibility to tune the architecture and morphology of the scaffolds, it is possible to mimic as much as possible the anatomical cell arrangement of the native tissues enabling optimal conditions for the regeneration of specific tissues.

Fedorovich et al. [36] developed a 3D fiber deposition (3DF) technique for the fabrication of heterogeneous hydrogel constructs. This novel technique allowed the control of fiber spacing and deposition angle, as well as the capability to dispense cells. The cell-leaden scaffolds were composed by two sections: (1) alginate with encapsulated chondrocytes and (2) alginate supplemented with biphasic calcium phosphate and HA with encapsulated bone marrow stem cells. The authors confirmed heterogeneous tissue formation, but, as expected, the use of alginate will not confer sufficient mechanical strength in future OC applications. Shim et al. [37] developed a multi-head tissue/organ building system (MtoBS) capable of dispensing a wide range of relevant biomaterials to produce 3D tissues or organs. That complex system was composed by six nozzles: two nozzles were used to dispense molten PCL, while four nozzles dispensed a liquid alginate hydrogel encapsulated with human osteoblast-derived cells or chondrocytes derived from human nasal septum. Therefore, PCL was used to enhance the mechanical properties of the constructs and the cell-leaden hydrogel was to confer biological cues to induce the regeneration process. The in vitro biological assays revealed cell viability maintenance of printed cells up to 7 days.

In another study, Cui et al. [38] combined a solution of poly(ethylene glycol) dimethacrylate (PEGDMA) with human chondrocytes to print scaffolds for osteochondral defects. This technique is based on a photo-polymerization reaction, where a photolytic cross-linker was used to form a hybrid cell-containing hydrogel. The cell-leaden structure revealed good integration with the surrounding tissue and the capability to maintain cell phenotype. Recently, cell-leaden osteochondral constructs composed of gelatin methacrylated hydrogel were fabricated. In this approach, scaffolds with high cell density and viability were achieved by the addition of mesenchymal stromal cells encapsulated in polylactic acid microcarriers. Additionally, the microcarrier encapsulation increased the stiffness of the printed constructs, as well as the cell adhesion and osteogenic differentiation. In a different approach, Cohen et al. [39] have developed an approach for in situ fabrication of an alginate scaffold. This novel strategy requires an adaptive system capable of performing real-time imaging, registration and path planning in order to directly print the material in the defect. In this study, the alginate cross-linking with calcium sulfate was initiated inside the printing cartridge and subsequently printed with the specific size and shape of defects formed in an ex vivo bovine femoral condyle. In a similar approach, Li et al. [40] applied 3D scanning and bioprinting for repairing osteochondral defects (Fig. 4). In that work, two different photopolymerized hydrogels were used as bioinks to fully restore the osteochondral defects. As well as in the previous study, the results suggested that 3D scanning and 3D bioprinting could provide a useful strategy for osteochondral regeneration. In situ SFF techniques have great potential for clinical applications. However, there are considerable challenges that need to be addressed regarding the material processing, printing resolution and printing conditions.
Fig. 4

Process of 3D bioprinting and photo-polymerization on osteochondral defect. Photo-polymerization was taken at the end of printing. a Repair of osteochondral defect through in situ 3D bioprinting with alginate hydrogel. b Exposure to UV light. c Alginate hydrogel that was printed to repair the osteochondral defect was transparent before photo-polymerization. df The color of alginate hydrogel turned milky white after being exposing to UV light in few seconds.

Reprinted with permission from Ref. [40]

Despite the challenges that still exist in SFF technique, 3D bioprinting can be considered a powerful tool for the development of cell-laden tissue constructs with suitable characteristics for OC tissue engineering.

In this review, three different SFF techniques (i.e., stereolithography, fused deposition modeling and 3D bioprinting) that have been widely used in OC tissue engineering applications are discussed. In our opinion, it cannot be said that there is a better or a worse technique, but we can mention that each one of the three SFF techniques presents some advantages and limitations (Table 1), and it is thus important to choose the most adequate technique for the envisioned application, which will also depend on the type of processing and material that will be used.
Table 1

Advantages and limitations of SFF techniques and examples of materials used in these techniques






High detailed constructs

Good surface finish

Requires post-curing

Possibility of shrinkage and curl

Limited biomaterials (photopolymers)

The need of support

In some cases, the difficulty of removing the support structures

Modified polypropylene fumarate

Modified polyethylene glycol (PEG)

Modified polyvinyl alcohol (PVA)

Modified polycaprolactone (PCL)

Methyl methacrylate (MMA)

Butyl methacrylate (BMA)

Fused deposition modeling

No post-curing

Variety of biomaterials

Easy to change the biomaterial


Low detailed constructs

Surface finish

Support structures are needed depending on the model design

In some cases, the difficulty of removing the support structures

Polycaprolactone (PCL)

Polyethylene glycol (PEG)

Polylactic acid (PLA)

Polyglycolic acid (PGA)

Poly(ethylene glycol)-terephthalate poly(butylene terephthalate) (PEGT/PBT)

Polyethylene-oxide-terephthalate/polybutylene-terephthalate (PEOT/PBT)

3D Bioprinting

Controllable cell spatial distribution

Better biological properties

Variety of biomaterials

Low detailed constructs


Sterilization of the printing environment

Ethical issues

Polycaprolactone (PCL)

Polyethylene glycol (PEG)




The SFF approaches have been revolutionizing scaffolds fabrication techniques in the OC tissue engineering field. In this review, three different SFF techniques have been described showing the most promising features for future applications, being one of these features the capability offered in terms of design control. Pore architecture, pore geometry, total porosity and cell density are some of the aspects that can be tuned using these SFF approaches. Probably, the 3D bioprinting is the most promising technique among the three, due to the possibility to control the cell spatial distribution in order to produce more complex constructs. Concerning the polymers used, over the last few years, a wide group of materials has been investigated, with the natural materials taking advantage over the synthetic materials due to their biocompatibility. Despite the important advances in this field, further investigation concerning material processing, printing resolution and polymers biocompatibility is necessary to translate the in vitro validated results to the clinics.



The authors would like to thank H2020-MSCA-RISE program, as this work is part of developments carried out in BAMOS project, funded from the European Union’s Horizon 2020 research and innovation program under grant agreement Nº 734156. The Portuguese Foundation for Science and Technology (FCT) distinctions attributed to J. Silva-Correia (IF/00115/2015) and J. Miguel Oliveira (IF/01285/2015) under the Investigator FCT program are greatly acknowledged. FCT/MCTES is also acknowledged for the PhD scholarship attributed to J. B. Costa (PD/BD/113803/2015).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Zhejiang University Press 2018

Authors and Affiliations

  1. 1.3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and BiomimeticsUniversity of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineBarco, GuimarãesPortugal
  2. 2.ICVS/3B’s–PT Government Associate LaboratoryBraga, GuimarãesPortugal
  3. 3.The Discoveries Centre for Regenerative and Precision MedicineHeadquarters at University of MinhoBarco, GuimarãesPortugal

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