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Uncovering proteomics changes of Penicillium expansum spores in response to decanal treatment by iTRAQ

  • Ting Zhou
  • Bishun Ye
  • Zhiqian Yan
  • Xiaohong Wang
  • Tongfei LaiEmail author
Original Article
  • 12 Downloads

Abstract

Exogenous decanal can significantly inhibit the germination and development of postharvest pathogen Penicillium expansum in vitro. Through an iTRAQ-based analysis, a global view of proteomic alteration of P. expansum spores under decanal treatment was acquired. A total of 246 up-regulated and 293 down-regulated differentially expressed proteins (DEPs) were identified. Among them, DEPs related to glutathione metabolism, ribosome, and oxidative phosphorylation pathway were noticed for their functional significance, large number and high rich value in pathway enrichment statistics. Further analysis found that, under decanal stress, expression of 9 genes corresponding to DEPs involved in the oxidative phosphorylation pathway showed a significantly decreasing trend, and activities of 3 crucial enzymes (NADH dehydrogenase, CoQ-Cytochrome c reductase, and F1F0-ATP synthetase) were inhibited. Also, a decreased in ATP content, reduction in the number of mitochondria, and weakening in carbohydrate consumption were detected. Based on these results, disturbance of oxidative phosphorylation would partly be responsible for the inhibitory effect of decanal on the growth of P. expansum. The findings would provide new insights into exploring the possible antifungal mechanisms of decanal.

Keywords

Penicillium expansum Decanal iTRAQ Oxidative phosphorylation 

Notes

Acknowledgments

This work was supported by the Zhejiang Provincial Natural Science Foundation of China (LY19C200022, LY18C150009) and the National Natural Science Foundation of China (NSFC31501810).

Funding information

This study was funded by the Zhejiang Provincial Natural Science Foundation of China (LY19C200022, LY18C150009) and the National Natural Science Foundation of China (NSFC31501810).

Compliance with ethical standards

Conflict of interest

All the authors declare that they have no conflict of interest.

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

42161_2020_486_MOESM1_ESM.xlsx (318 kb)
ESM 1 (XLSX 317 kb)
42161_2020_486_MOESM2_ESM.docx (137 kb)
ESM 2 (DOCX 137 kb)

References

  1. AI-Attar S, Yu YJ, Pinkse M, Hoeser J, Friedrich T, Bald D, de Vries S (2016) Cytochrome bd display significant quinol peroxidase activity. Sci Rep-UK 6:2763Google Scholar
  2. Atarés L, Chiralt A (2016) Essential oils as additives in biodegradable films and coating for active food packaging. Trends Food Sci Technol 48:51–62CrossRefGoogle Scholar
  3. Barad S, Sionov E, Prusky D (2016) Role of patulin in post-harvest diseases. Fungal Biol Rev 30:24–32CrossRefGoogle Scholar
  4. Calo JR, Grandall PG, O’Bryan CA, Ricke SC (2015) Essential oils as antimicrobials in food systems-A review. Food Control 54:111–119CrossRefGoogle Scholar
  5. Chaban Y, Boekema EJ, Dudkina N (2014) Structures of mitochondrial oxidative phosphorylation supercomplexes and mechanisms for their stabilization. BBA-Bioenergetics 1837:418–426CrossRefGoogle Scholar
  6. Chang PK, Scharfenstein LL, Mack B, Yu JJ, Ehrlich KC (2014) Transcriptomic profiles of Aspergillus flavus CA42, a strain that produces small sclerotia, by decanal treatment and after recovery. Fungal Genet Biol 68:39–47CrossRefGoogle Scholar
  7. Chen L, Zhao B, Fan ZJ, Liu XM, Wu QF, Li HP, Wang HX (2018) Synthesis of novel 3,4-chloroisothiazole-base imidazoles as fungicides and evaluation of their mode of action. J Agric Food Chem 66:7319–7327CrossRefGoogle Scholar
  8. Daniel CK, Lennoxa CL, Vriesb FA (2015) In vivo application of garlic extracts in combination with clove oil to prevent postharvest decay caused by Botrytis cinerea, Penicillium expansum and Neofabraea alba on apples. Postharvest Biol Technol 99:88–92CrossRefGoogle Scholar
  9. Das D, Samanta D, Das A, Chosh J, Bhattacharya A, Basu A, Chakrabarti A, Gupta CD (2010) Ribosome: the structure-function relation and a new paradigm to the protein folding problem. Isr J Chem 50:109–116CrossRefGoogle Scholar
  10. De Oliveira KÁR, Berger LRR, Araújo SAD, Câmara MPS, Souza ELD (2017) Synergistic mixtures of chitosan and Mentha piperita L. essential oil to inhibit Colletotrichum species and anthracnose development in mango cultivar Tommy Atkins. Food Microbiol 66:96–103CrossRefGoogle Scholar
  11. Dimroth P, Kaim G, Matthey U (2000) Crucial role of the membrane potential for ATP synthesis by F1F0 ATP synthases. J Exp Biol 203:51–59PubMedGoogle Scholar
  12. Gabaldón T (2010) Peroxisome diversity and evolution. Philos. T R Soc B 365:765–773CrossRefGoogle Scholar
  13. Grinberg AV, Hannemann F, Schiffler B, Müller J, Heinemann U (2000) Adrenodoxin: structure, stability, and electron transfer properties. Proteins 40:590–612CrossRefGoogle Scholar
  14. Hatefi Y (1985) The mitochondrial electron transport and oxidative phosphorylation system. Annu Rev Biochem 54:1051–1069CrossRefGoogle Scholar
  15. Kühlbrandt W, Davies KM (2016) Rotary ATPases: a new twist to an ancient machine. Trends Biochem Sci 41:106–116CrossRefGoogle Scholar
  16. Lai TF, Wang Y, Fan YY, Zhou YY, Bao Y, Zhou T (2017) The response of growth and patulin production of postharvest pathogen Penicillium expansum to exogenous potassium phosphite treatment. Int J Food Microbiol 244:1–10CrossRefGoogle Scholar
  17. Li BQ, Zong YY, Du ZL, Chen Y, Zhang ZQ, Qin GZ, Zhao WM, Tian SP (2015) Genomic characterization reveals insights into patulin biosynthesis and pathogenicity in Penicillium species. Mol Plant Microbe In 28:635–647CrossRefGoogle Scholar
  18. Li YH, Shao XF, Xu JY, Wei YY, Xu F, Wang HW (2017) Tea tree oil exhibits antifungal activity against Botrytis cinerea by affecting mitochondria. Food Chem 234:62–67CrossRefGoogle Scholar
  19. Liu T, Zhou J, Cui HJ, Li PF, Luo JK, Li T, He F, Wang Y, Tang T (2019) iTRAQ-based quantitative proteomics reveals the neuroprotection of rhubarb in experimental intracerebral hemorrhage. J Ethnopharmacol 232:244–254CrossRefGoogle Scholar
  20. Mahboubi M, Feizabadi MM (2009) Antimicrobial activity of ducrosia anethifolia essential oil and main component, decanal against methicillin-resistant and methicillin-susceptible Staphylococcus aureus. J Essent Oil Bear Pl 12:574–579CrossRefGoogle Scholar
  21. Morales H, Marín S, Ramos AJ, Sanchis V (2010) Influence of post-harvest technologies applied during cold storage of apples in Penicillium expansum growth and patulin accumulation: a review. Food Control 21:953–962CrossRefGoogle Scholar
  22. Muroi M, Osada H (2019) Proteomic profiling for target identification of biologically active small molecule using 2D DIGE. Methods Mol Biol 1888:127–139CrossRefGoogle Scholar
  23. Page CC, Moser CC, Chen XX, Dutton PL (1999) Natural engineering principles of electron tunneling in biological oxidation-reduction. Nature 402:47–52CrossRefGoogle Scholar
  24. Palou L, Ali A, Fallik E, Romanazzi G (2016) GRAS, plant- and animal-derived compounds as alternatives to conventional fungicides for the control of postharvest diseases of fresh horticultural produce. Postharvest Biol Technol 122:41–52CrossRefGoogle Scholar
  25. Pompella A, Visvikis A, Paolicchi A, Tata VD, Casini AF (2003) The changing faces of glutathione, a cellular protagonist. Biochem Pharmacol 66:1499–1503CrossRefGoogle Scholar
  26. Qin GZ, Tian SP, Chan ZL, Li BQ (2006) Crucial role of antioxidant proteins and hydrolytic enzymes in pathogenicity of Penicillium expansum. Mol Cell Proteomics 6:425–438CrossRefGoogle Scholar
  27. Reyes-Prieto A, Barquera B, Juárez O (2014) Origin and evolution of the sodium-pumping NADH: ubiquinone oxidoreductase. PLoS One 9:e96696CrossRefGoogle Scholar
  28. Rich P (2003) The molecular machinery of Keilin’s respiratory chain. Biochem Soc T 31:1095–1105CrossRefGoogle Scholar
  29. Senior AE (1988) ATP synthesis by oxidative phosphorylation. Physiol Rev 68:177–231CrossRefGoogle Scholar
  30. Soares BV, Morais SM, Fontenelle RODS, Queiroz VA, Vila-Nova NS, Pereira CMC, Brito ES, Neto MAS, Brito EHS, Cavalcante CSP, Castelo-Branco DSCM, Rocha MFG (2012) Antifungal activity, toxicity and chemical composition of the essential oil of Coriandrum sativum L. fruits. Molecules 17:8439–8448CrossRefGoogle Scholar
  31. Tao NG, Jia L, Zhou HE (2014) Anti-fungal activity of Citrus reticulate Blanco essential oil against Penicillium italicum and Penicillium digitatum. Food Chem 153:265–271CrossRefGoogle Scholar
  32. Tian J, Zeng XB, Feng ZZ, Miao XM, Peng X, Wang YW (2014) Zanthoxylum molle Rehd. essential oil as a potential natural preservative in management of Aspergillus flavus. Ind Crop Prod 60:151–159CrossRefGoogle Scholar
  33. Yoshikawa S, Shimada A (2015) Reaction mechanism of cytochrome c oxidase. Chem Rev 115:1936–1989CrossRefGoogle Scholar
  34. Zhao B, Wang HX, Fan ZJ, Wu QF, Guo XF, Zhang NL, Yang DY, Yu B, Zhou S (2018) Mode of action for a new potential fungicide candidate, 3-(4-Methyl-1,2,3-thiadiazolyl)-6-trichloromethyl-[1,2,4]-triazolo-[3,4-b][1,3,4]-thiadiazole by iTRAQ. Food Agric Immunol 30:533–547CrossRefGoogle Scholar
  35. Zhou T, Wang XH, Ye BS, Shi L, Bai XL, Lai TF (2018) Effects of essential oil decanal on growth and transcriptome of the postharvest fungal pathogen Penicillium expansum. Postharvest Biol Technol 145:203–212CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020

Authors and Affiliations

  1. 1.Hangzhou Key Laboratory for Safety of Agricultural Products, College of Life and Environmental ScienceHangzhou Normal UniversityHangzhouChina
  2. 2.Research Centre for Plant RNA Signaling, College of Life and Environmental ScienceHangzhou Normal UniversityHangzhouChina

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