Uncovering proteomics changes of Penicillium expansum spores in response to decanal treatment by iTRAQ

  • Ting Zhou
  • Bishun Ye
  • Zhiqian Yan
  • Xiaohong Wang
  • Tongfei LaiEmail author
Original Article


Exogenous decanal can significantly inhibit the germination and development of postharvest pathogen Penicillium expansum in vitro. Through an iTRAQ-based analysis, a global view of proteomic alteration of P. expansum spores under decanal treatment was acquired. A total of 246 up-regulated and 293 down-regulated differentially expressed proteins (DEPs) were identified. Among them, DEPs related to glutathione metabolism, ribosome, and oxidative phosphorylation pathway were noticed for their functional significance, large number and high rich value in pathway enrichment statistics. Further analysis found that, under decanal stress, expression of 9 genes corresponding to DEPs involved in the oxidative phosphorylation pathway showed a significantly decreasing trend, and activities of 3 crucial enzymes (NADH dehydrogenase, CoQ-Cytochrome c reductase, and F1F0-ATP synthetase) were inhibited. Also, a decreased in ATP content, reduction in the number of mitochondria, and weakening in carbohydrate consumption were detected. Based on these results, disturbance of oxidative phosphorylation would partly be responsible for the inhibitory effect of decanal on the growth of P. expansum. The findings would provide new insights into exploring the possible antifungal mechanisms of decanal.


Penicillium expansum Decanal iTRAQ Oxidative phosphorylation 



This work was supported by the Zhejiang Provincial Natural Science Foundation of China (LY19C200022, LY18C150009) and the National Natural Science Foundation of China (NSFC31501810).

Funding information

This study was funded by the Zhejiang Provincial Natural Science Foundation of China (LY19C200022, LY18C150009) and the National Natural Science Foundation of China (NSFC31501810).

Compliance with ethical standards

Conflict of interest

All the authors declare that they have no conflict of interest.

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

42161_2020_486_MOESM1_ESM.xlsx (318 kb)
ESM 1 (XLSX 317 kb)
42161_2020_486_MOESM2_ESM.docx (137 kb)
ESM 2 (DOCX 137 kb)


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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2020

Authors and Affiliations

  1. 1.Hangzhou Key Laboratory for Safety of Agricultural Products, College of Life and Environmental ScienceHangzhou Normal UniversityHangzhouChina
  2. 2.Research Centre for Plant RNA Signaling, College of Life and Environmental ScienceHangzhou Normal UniversityHangzhouChina

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