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Growth and physiological responses of selected pakchoi varieties to Plasmodiophora brassicae infection

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Abstract

Clubroot disease, caused by Plasmodiophora brassicae, is a serious soil-borne disease of Brassica crops worldwide. Pakchoi is one of the most important cruciferous vegetable crops and commonly infected by P. brassicae. To date, the physiological changes and mechanism of pakchoi in response to P. brassicae are largely unknown. In this study, seedlings of two pakchoi cultivars, cv. Xinxiaqing (a clubroot-susceptible cultivar) and Yanlong (a less susceptible cultivar than Xinxiaqing), were inoculated with P. brassicae. Results showed that infected roots were enlarged and changed into club shape galls. Fresh root weight, relative electrical conductivity, the activity of lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH) in infected roots were significantly induced. In contrast, a significant reduction of root length, root function, and the activity of succinic dehydrogenase (SDH), catalase (CAT), ascorbate peroxidase (APX), and superoxide dismutase (SOD) were observed in diseased roots. Interestingly, activities of malate dehydrogenase (MDH) and peroxidase (POD) in S1 and S2 plant roots were increased and decreased in S3 plant roots. Additionally, infected Xinxiaqing roots showed more serious disease symptoms than Yanlong, and the antioxidant enzyme activities were lowered in Xinxiaqing roots compared with those in infected Yanlong roots, indicating the importance of these enzymes in plant resistance against clubroot disease.

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Acknowledgments

This work was supported by Agriculture Research System of Shanghai, China (Grant No. 201903) and National Key R&D Program of China 2017YFD0101803. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Correspondence to Yuying Zhu.

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Zhu, H., Li, X., Khalid, M. et al. Growth and physiological responses of selected pakchoi varieties to Plasmodiophora brassicae infection. J Plant Pathol (2020). https://doi.org/10.1007/s42161-019-00470-9

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Keywords

  • Clubroot disease
  • Disease index
  • Root growth
  • Antioxidant enzyme activities
  • Respiratory enzyme activities