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Development and validation of RT-PCR multiplex detection of grapevine viruses and viroids in the Czech Republic

  • Marcela Komínková
  • Petr KomínekEmail author
Short Communication
  • 28 Downloads

Abstract

A multiplex RT-PCR reaction was developed and validated for the simultaneous detection of two viruses and two viroids: grapevine fleck virus, grapevine Pinot gris virus, grapevine yellow speckle viroid 1, and hop stunt viroid. The multiplex reaction specificity was verified on a set of 210 grapevines from the Czech Republic. The results showed the occurrence of hop stunt viroid in 94.3% of the examined plants, while incidence of the other pathogens was lower: grapevine Pinot gris virus (64.8%), grapevine yellow speckle viroid 1 (52.4%), and grapevine fleck virus (15.2%).

Keywords

Multiplex Detection Grapevine fleck virus Grapevine Pinot gris virus Grapevine yellow speckle viroid 1 Hop stunt viroid 

Notes

Acknowledgements

The authors wish to thank Peter Lemkin for improvements in the English.

Funding information

This work was supported by institutional support MZE-RO0418 from the Ministry of Agriculture of the Czech Republic.

Compliance with ethical standards

The authors declare they carefully followed the COPE guidelines.

The authors declare that they have no conflicts of interest.

Supplementary material

42161_2019_452_MOESM1_ESM.xlsx (24 kb)
ESM 1 (XLSX 24 kb)

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Plant Virology and PhytoplasmatologyCrop Research InstitutePraha 6 - RuzyněCzech Republic

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