Development and validation of RT-PCR multiplex detection of grapevine viruses and viroids in the Czech Republic
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A multiplex RT-PCR reaction was developed and validated for the simultaneous detection of two viruses and two viroids: grapevine fleck virus, grapevine Pinot gris virus, grapevine yellow speckle viroid 1, and hop stunt viroid. The multiplex reaction specificity was verified on a set of 210 grapevines from the Czech Republic. The results showed the occurrence of hop stunt viroid in 94.3% of the examined plants, while incidence of the other pathogens was lower: grapevine Pinot gris virus (64.8%), grapevine yellow speckle viroid 1 (52.4%), and grapevine fleck virus (15.2%).
KeywordsMultiplex Detection Grapevine fleck virus Grapevine Pinot gris virus Grapevine yellow speckle viroid 1 Hop stunt viroid
The authors wish to thank Peter Lemkin for improvements in the English.
This work was supported by institutional support MZE-RO0418 from the Ministry of Agriculture of the Czech Republic.
Compliance with ethical standards
The authors declare they carefully followed the COPE guidelines.
The authors declare that they have no conflicts of interest.
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