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Colletotrichum fructicola STE50 is required for vegetative growth, asexual reproduction, appressorium formation, pathogenicity and the response to external stress

  • Yan-ying Chen
  • Jun-ang Liu
  • Shi-qiang Jiang
  • He Li
  • Guo-ying ZhouEmail author
Original Article

Abstract

Colletotrichum fructicola is a fungal pathogen that causes Camellia oleifera anthracnose. Mitogen-activated protein kinase cascade signaling regulates plants defenses and is modulated by Ste50, a scaffold protein that mediates the interaction of Ste11 and Ste7. In this study, the Saccharomyces cerevisiae STE50 homolog CfSTE50 in C. fructicola was identified and disrupted. CfSTE50 encodes a 482 amino acid protein. The mutants showed significant differences in conidial yields and appressorium failed to form. Vegetative growth also decreased compared to wild-type strains. Inoculation with conidial suspensions showed that the virulence of the mutant on wounded leaves was significantly impaired. Further analysis showed that the targeted deletion of CfSTE50 resulted in enhanced sensitivity to external peroxide stress, but reduced sensitivity to cell wall stress. These results provide evidence that CfSte50 regulates the growth and development of C. fructicola and regulates asexual reproduction, appressorium formation, pathogenicity and the response to external stresses.

Keywords

Anthracnose Gene function Fungus pathogen Tea oil camellia 

Notes

Acknowledgments

This study was funded by the National Program on Key Research Project of China (grant number 2017YFD0600103-3) and Scientific Innovation Fund for Graduate of Central South University of Forestry and Technology (grant number 20183031).

Funding information

This study was funded by the National Program of the Key Research Project of China (grant number 2017YFD0600103–3) and Scientific Innovation Fund for the Graduate of Central South University of Forestry and Technology (grant number 20183031).

Compliance with ethical standards

Conflict of interest

All authors declare no conflicts of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

42161_2019_422_MOESM1_ESM.docx (21 kb)
Online Resource 1 (DOCX 21 kb)
42161_2019_422_Fig7_ESM.png (37 kb)
Online Resource 2

Minimum inhibitory concentration of hygromycin B and bleomycin on CFLH16 (PNG 37 kb)

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High Resolution Image (TIF 49 kb)
42161_2019_422_MOESM3_ESM.docx (22 kb)
Online Resource 3 (DOCX 21 kb)

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  • Yan-ying Chen
    • 1
    • 2
    • 3
  • Jun-ang Liu
    • 1
    • 2
    • 3
  • Shi-qiang Jiang
    • 1
    • 2
    • 3
  • He Li
    • 1
    • 2
    • 3
  • Guo-ying Zhou
    • 1
    • 2
    • 3
    Email author
  1. 1.Key Laboratory of National Forestry and Grassland Administration on Control of Artificial Forest Diseases and Pests in South ChinaCentral South University of Forestry and TechnologyChangshaChina
  2. 2.Hunan Provincial Key Laboratory for Control of Forest Diseases and PestsCentral South University of Forestry and TechnologyChangshaChina
  3. 3.Key Laboratory for Non-wood Forest Cultivation and Conservation of Ministry of EducationCentral South University of Forestry and TechnologyChangshaChina

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