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First report of tobacco vein banding mosaic virus infecting Solanum lyratum Thunb in China

  • Jian Zhang
  • Yong-Yuan Cheng
  • Lei Yang
  • Xin Sun
  • Jing Yu
  • Quan-You LuEmail author
Disease Note
  • 37 Downloads

Keywords

Solanum lyratum Thunb Vein banding and mosaic symptoms Tobacco vein banding mosaic virus 

Solanum lyratum Thunb, a species of the genus Solanum in the family Solanaceae, is a common Chinese herbal medicine. Tobacco vein banding mosaic virus (TVBMV) is a member of the genus Potyvirus and was first discovered in Taiwan in 1964 (Habera et al. 1994). In July 2018, several S. lyratum plants growing naturally along a roadside exhibited virus-like symptoms including dark green vein banding and mosaic in Zhenjiang, Jiangsu province, China. The crude sap from the symptomatic leaves of S. lyratum was rubbed onto leaves of Nicotiana benthamiana plants, resulting in mild downward leaf curling and mild mosaic symptoms at 9 days post inoculation (dpi) and severe downward leaf curling, yellowing and plant stunting at 20 dpi, indicating mechanical transmission of a virus. Mock-inoculated N. benthamiana plants did not exhibit any symptoms at 9 or 20 dpi. Double-stranded (ds) RNA was extracted from the symptomatic leaves of S. lyratum (Morris and Dodds 1979), and used as a template for sequence-independent amplification (SIA) (Agindotan et al. 2010). Polymerase chain reaction (PCR) products were ligated into pUCm-T Vector (Sangon, Shanghai, China) and sequenced (SunYa, Shanghai, China). BLAST analysis showed that the nucleotide sequence (nt) of seven fragments were 92–94% identical to a TVBMV isolate from Shandong, China (JN630469). The presence of TVBMV was further confirmed in the symptomatic S. lyratum and N. benthamiana plants inoculated with crude sap by reverse transcription (RT) PCR using virus-specific primers (forward, 5’-GGATATTGGTGAGCAGATGC-3′; and reverse, 5’-CGTTTTCGATGCACCACACCATC-3′) designed on the sequences obtained by SIA. The expected 1109 nt product was not amplified from the asymptomatic or the mock-inoculated N. benthamiana plants. Subsequently, the complete genome of this isolate (tentatively named TVBMV-lyr) was determined by sequencing RT-PCR products obtained using degenerate TVBMV primers designed from the results of multiple sequence alignments of TVBMV isolates along with TMV-lyr-specific primers designed from sequences obtained by SIA. The complete genomic sequence of TVBMV-lyr was deposited in GenBank as accession number MH898883. It is 9570 nt in length and shares the highest nucleotide identity (94.32%) with TVBMV-SDZC1 (HQ396793). To our knowledge, this is the first report of TVBMV infection in S. lyratum.

Notes

Acknowledgements

This work was partially granted by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No. 18KJA210001).

Supplementary material

42161_2019_419_MOESM1_ESM.jpg (62 kb)
ESM 1 Fig. 1 Virus-like symptoms exhibiting in Solanum lyratum Thunb (A) and comparison between the healthy (left) and diseased (right) leaves (B) (JPG 61 kb)
42161_2019_419_MOESM2_ESM.jpg (72 kb)
ESM 2 Fig. 2 Symptoms expression 9 dpi (upper panel) and 20 dpi on Nicotiana benthamiana plants inoculated with crude sap from symptomatic S. lyratum leaves. The dpi is the abbreviation for days postinoculation. Panel A shows the symptom of plant stunting. Panel B shows the symptoms of severe downward leaf curling and yellowing (JPG 72 kb)

References

  1. Habera LF, Berger PH, Reddick BB (1994) Molecular evidence from 3′-terminus sequence-analysis that tobacco vein-banding mosaic virus is a distinct member of the Potyvirus group. Arch Virol 138:27–38CrossRefGoogle Scholar
  2. Agindotan BO, Ahonsi MO, Domier LL, Gray ME, Bradley CA (2010) Application of sequence-independent amplification (SIA) for the identification of RNA viruses in bioenergy crops. J Virol Methods 169:119–128CrossRefGoogle Scholar
  3. Morris TJ, Dodds JA (1979) Isolation and analysis of double stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69(8):854–858CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Sericultural Research InstituteJiangsu University of Science and TechnologyZhenjiangPeople’s Republic of China
  2. 2.College of BiotechnologyJiangsu University of Science and TechnologyZhenjiangPeople’s Republic of China
  3. 3.Key Laboratory of Genetic Improvement of Silkworm and Mulberry, Ministry of Agriculture and Rural Affairs, Sericultural Research InstituteChinese Academy of Agricultural SciencesZhenjiangPeople’s Republic of China

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