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Characterization of a severe isolate of papaya ringspot virus from papaya in western India

  • Asawari Gorane
  • Raj Verma
  • Archana Naik
  • Tukaram Nikam
  • Avinash Ade
  • Gagan Mahapatro
  • Savarni TripathiEmail author
Short Communication
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Abstract

Papaya ringspot virus (PRSV), the causative agent of a devastating and widespread ringspot disease in papaya (Carica papaya L.), belongs to the family Potyviridae. Here, we report the first complete genome characterization of a severe PRSV isolate (PRSV-Pune; GenBank accession No. MH311882) from papaya in the Pune district, western India. The genome size of PRSV-Pune is 10,326 nucleotides (nt), excluding the poly-A tail, encoding a polyprotein of 3345 amino acids (aa). The PRSV-Pune showed identity ranging from 82 to 86% and 87–90% at the nucleotide and amino acid levels, respectively, with 29 complete PRSV-P genomes from all over the world. Variations in amino acid composition at and around two polyprotein cleavage sites of PRSV-Pune were observed: MEQY/S instead of MEQY/N in P1/HC-Pro and DYHQ/N instead of VYHQ/S in CI/6K2. Phylogenetic analysis revealed the clustering of PRSV-Pune, along with the majority of Indian PRSV isolates, with isolates of American lineage. Putative recombination events in P1, HC-Pro, CI, NIb and CP coding regions were detected in PRSV-Pune. Negative selection pressure predicted in all cistrons indicates purifying selection. Our results highlight unique attributes of the severe PRSV-Pune from papaya.

Keywords

Papaya ringspot virus Complete genome Papaya Phylogenetic analysis Recombination 

Notes

Acknowledgements

This work was supported by the Savitribai Phule Pune University and the ICAR-Indian Agricultural Research Institute (in-house fund).

Funding

This work was supported by the Savitribai Phule Pune University and the ICAR-Indian Agricultural Research Institute (in-house grant).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

42161_2019_340_MOESM1_ESM.docx (44 kb)
ESM 1 (DOCX 43 kb)

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  • Asawari Gorane
    • 1
    • 2
  • Raj Verma
    • 2
  • Archana Naik
    • 1
  • Tukaram Nikam
    • 1
  • Avinash Ade
    • 1
  • Gagan Mahapatro
    • 2
  • Savarni Tripathi
    • 2
    Email author
  1. 1.Department of BotanySavitribai Phule Pune UniversityPuneIndia
  2. 2.ICAR-Indian Agricultural Research InstitutePuneIndia

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