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Journal of Plant Pathology

, Volume 101, Issue 4, pp 1271–1272 | Cite as

First report of sheath rot caused by Fusarium proliferatum on Pisang Awak Banana (Musa ABB) in China

  • Sui-Ping Huang
  • Ji-Guang Wei
  • Tang-Xun Guo
  • Qi-Li Li
  • Li-Hua Tang
  • Jian-You MoEmail author
  • Ji-Feng Wei
  • Xiao-Bing Yang
Open Access
Disease Note
  • 321 Downloads

Keywords

Pisang Awak banana Sheath rot Fusarium proliferatum 

Pisang Awak banana (Musa ABB) is the second largest banana variety cultivated in China. A severe sheath disease of Pisang Awak banana with disease incidence of over 90% was observed in a plantation in Du’an County, Guangxi, China in 2017. Black punctiform lesions first appeared on the sheaths connecting petioles to stems. As lesions expanded, the leaves wilted and broke off near the base, causing the sheaths to die and rot away. Symptomatic plants had smaller stems and reduced yields. A fungus was consistently isolated from the surface-sterilized sheath samples. The isolate XJSF had abundant aerial white mycelia on potato dextrose agar (PDA). Microconidia on PDA were aseptate, club shaped, 4.25 to 8.57 × 1.7 to 3.21 μm (average 5.90 × 2.34 μm). Macroconidia on carnation leaf agar were slender, almost straight, 3–4 septate, 32.9 to 57.6 × 2.51 to 4.55 μm (average 44.53 × 3.57 μm). The internal transcribed spacer (ITS) of the ribosomal DNA sequences (GenBank accession number MF083155) and the translation elongation factor 1-alpha (EF-1α) gene sequences (MF083156) (O’Donnell et al. 1998) were deposited in GenBank. BlastN searches showed 100% and 99% identity to F. proliferatum strains U34558 and AF160280.1, respectively. The fungus was therefore identified as Fusarium proliferatum, based on morphological (Leslie and Summerell 2006) and ITS and EF-1α sequences analysis. Pathogenicity tests were performed on the sheaths of healthy Pisang Awak banana plantlets with a conidial suspension (1 × 106 conidia/ml) of the XJSF isolate according to the method of Huang et al. (2017). After three months, the whole sheaths of inoculated plants were rotted while only slight scars formed on the control plants. Fungi re-isolated from the lesions were identified as F. proliferatum based on morphology. To our knowledge, this is the first report of sheath rot on banana (Musa ABB) caused by F. proliferatum world-wide.

Notes

Acknowledgements

This work was financed by the National Key R&D Program of China (Grant No. 2016YFC1202100), Science and Technology Major Project of Guangxi (Grant No. AA18118028) and Planned Project of Scientific Research and Technology Development in Qingxiu District, Nanning City (Award Number 2017040).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

The authors declare that no human participants and animals were involved in this study.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

42161_2019_329_MOESM1_ESM.png (994 kb)
Fig. 1. (A-C) Symptoms of sheath rot caused by Fusarium proliferatum on Pisang Awak banana in a plantation in Guangxi, China; (D) diseased sheaths having a purplish red color; (E) symptoms of sheath rot caused by F. proliferatum on Pisang Awak banana three months after inoculation; (F) slight scars of inoculation of non- pathogenic F. oxysporum on Pisang Awak banana as control three months after inoculation. (PNG 994 kb)
42161_2019_329_MOESM2_ESM.png (134 kb)
Fig. 2. (A) Morphology of colony; (B) microconidia and macroconidia of F. proliferatum grown on carnation leaf agar after 21 days at 25°C. Bars=10 μm. (PNG 134 kb)

References

  1. Huang SP, Li ZL, Wei JG et al (2017) First report of stem canker caused by Fusarium solani on Tectona grandis in China. Plant Dis 101(12):2148–2148CrossRefGoogle Scholar
  2. Leslie JF, Summerell BA (2006) The Fusarium laboratory manual. Blackwell Publishing, OxfordCrossRefGoogle Scholar
  3. O’Donnell K, Kistler HC, Cigelnik E et al (1998) Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci 95(5):2044–2049CrossRefGoogle Scholar

Copyright information

© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.College of AgricultureGuangxi UniversityNanningChina
  2. 2.Plant Protection Research InstituteGuangxi Academy of Agricultural SciencesNanningChina
  3. 3.Department of Scientific Research ManagementGuangxi College of EducationNanningChina
  4. 4.Iowa State UniversityAmesUSA

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