Journal of Plant Pathology

, Volume 101, Issue 4, pp 1187–1193 | Cite as

Length of poly(A) tail affects transcript infectivity of three ZYMV symptom variants differing at only five amino acid positions

  • Boram Kim
  • In-Sook Cho
  • Ik-Hyun Kim
  • Go-Woon Choi
  • Hye-Kyoung Ju
  • Wen-Xing Hu
  • June-Pyo Oh
  • Jung-Kyu Kim
  • Eunyong Seo
  • Leslie L. Domier
  • John HammondEmail author
  • Hyoun-Sub LimEmail author
Short Communication


The incidence of plant virus diseases infecting important cucurbit vegetables in Korea has increased as new isolates have been introduced, associated with warming temperatures and vector movement caused by climate change. Transcript infectivity of full-length infectious clones of three new ZYMV isolates was dependent upon the length of the poly(A) tract; transcripts with 55 A residues were inefficiently infectious, whereas 60 A residues resulted in highly efficient infection and significantly reduced time to production of systemic symptoms. Sequences of isolates BR1 (MH042024), BR2 (MH042025), and BR3 (MH042026) showed 99% pair-wise identity and differed at only five amino acid positions in: HC-Pro (D134N in BR2), CI (F31 L in BR1), and 6 K2 (A24V in BR3), and two positions in NIb (T300S in BR2, H429Q in BR3). Cucurbita pepo plants inoculated with transcripts of clones with these amino acid differences showed symptoms that ranged from mild to severe. Phylogenetic analysis of these new ZYMV isolates with previously characterized isolates indicated that the new isolates had 87.8–97.5% identity to other ZYMV isolates and were most closely related to recent ZYMV isolates from Australia and Spain.


Potyvirus Zucchini yellow mosaic virus (ZYMV) Full-length infectious cDNA clone Poly(A) tail 



This work was supported by a grant from the Next-Generation Biogreen 21 Program (PJ01365501), Rural Development Administration, Republic of Korea.

Compliance with ethical standards

Conflict of interest

Hyoun-Sub Lim on behalf of all authors, who collaborate under formal inter-institutional agreements declares that she or he has no conflict of interest. (Boram Kim declares that she has no conflict of interest. In-Sook Cho declares that she has no conflict of interest. Ik-Hyun Kim declares that he has no conflict of interest. Go-Woon Choi declares that she has no conflict of interest. Hye-Kyoung Ju declares that she has no conflict of interest. Wen-Xing Hu declares that he has no conflict of interest. June-Pyo Oh declares that he has no conflict of interest. Jung-Kyu Kim declares that he has no conflict of interest. Eunyong Seo declares that she has no conflict of interest. Leslie L Domier declares that he has no conflict of interest. John Hammond declares that he has no conflict of interest. Hyoun-Sub Lim declares that he has no conflict of interest).

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Department of Applied Biology, College of Agriculture and Life SciencesChungnam National UniversityDaejeonSouth Korea
  2. 2.Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal ScienceRural Development AdministrationWanjuSouth Korea
  3. 3.United States Department of Agriculture – Agricultural Research Service, Department of Crop SciencesUniversity of Illinois at Urbana-ChampaignChampaignUSA
  4. 4.United States Department of Agriculture – Agricultural Research ServiceUnited States National Arboretum, Floral and Nursery Plants Research UnitBeltsvilleUSA

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