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Journal of Plant Pathology

, Volume 101, Issue 4, pp 1187–1193 | Cite as

Length of poly(A) tail affects transcript infectivity of three ZYMV symptom variants differing at only five amino acid positions

  • Boram Kim
  • In-Sook Cho
  • Ik-Hyun Kim
  • Go-Woon Choi
  • Hye-Kyoung Ju
  • Wen-Xing Hu
  • June-Pyo Oh
  • Jung-Kyu Kim
  • Eunyong Seo
  • Leslie L. Domier
  • John HammondEmail author
  • Hyoun-Sub LimEmail author
Short Communication
  • 67 Downloads

Abstract

The incidence of plant virus diseases infecting important cucurbit vegetables in Korea has increased as new isolates have been introduced, associated with warming temperatures and vector movement caused by climate change. Transcript infectivity of full-length infectious clones of three new ZYMV isolates was dependent upon the length of the poly(A) tract; transcripts with 55 A residues were inefficiently infectious, whereas 60 A residues resulted in highly efficient infection and significantly reduced time to production of systemic symptoms. Sequences of isolates BR1 (MH042024), BR2 (MH042025), and BR3 (MH042026) showed 99% pair-wise identity and differed at only five amino acid positions in: HC-Pro (D134N in BR2), CI (F31 L in BR1), and 6 K2 (A24V in BR3), and two positions in NIb (T300S in BR2, H429Q in BR3). Cucurbita pepo plants inoculated with transcripts of clones with these amino acid differences showed symptoms that ranged from mild to severe. Phylogenetic analysis of these new ZYMV isolates with previously characterized isolates indicated that the new isolates had 87.8–97.5% identity to other ZYMV isolates and were most closely related to recent ZYMV isolates from Australia and Spain.

Keywords

Potyvirus Zucchini yellow mosaic virus (ZYMV) Full-length infectious cDNA clone Poly(A) tail 

Notes

Acknowledgements

This work was supported by a grant from the Next-Generation Biogreen 21 Program (PJ01365501), Rural Development Administration, Republic of Korea.

Compliance with ethical standards

Conflict of interest

Hyoun-Sub Lim on behalf of all authors, who collaborate under formal inter-institutional agreements declares that she or he has no conflict of interest. (Boram Kim declares that she has no conflict of interest. In-Sook Cho declares that she has no conflict of interest. Ik-Hyun Kim declares that he has no conflict of interest. Go-Woon Choi declares that she has no conflict of interest. Hye-Kyoung Ju declares that she has no conflict of interest. Wen-Xing Hu declares that he has no conflict of interest. June-Pyo Oh declares that he has no conflict of interest. Jung-Kyu Kim declares that he has no conflict of interest. Eunyong Seo declares that she has no conflict of interest. Leslie L Domier declares that he has no conflict of interest. John Hammond declares that he has no conflict of interest. Hyoun-Sub Lim declares that he has no conflict of interest).

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Blua MJ, Perring TM (1989) Effect of zucchini yellow mosaic-virus on development and yield of cantaloupe (Cucumis melo). Plant Dis 73:317–320CrossRefGoogle Scholar
  2. Cho SW (2018) Nonsan City, crop virus alert request for management of control of insect pests. Hungcheong message Web. http://www.ccmessage.kr/news/articleView.html?idxno=3762. Accessed 18 June 2018
  3. Choi GY (2017) Recent changes in the North Pacific Ocean air pollution affecting the subtropical nation in Korea. Korean Geographical Society Conference 6:73–73Google Scholar
  4. Choi SK, Yoon JY, Ryu KH, Choi JK (2002) First report of zucchini yellow mosaic virus on hollyhock (Althaea rosea). Plant Pathol J 18:121–125CrossRefGoogle Scholar
  5. Choi SK, Yoon JY, Choi GS (2015) Biological and molecular characterization of a Korean isolate of cucurbit aphid-borne yellows virus infecting Cucumis species in Korea. Plant Pathol J 31:371–378CrossRefGoogle Scholar
  6. Daugherty PM, Zeilinger RA, Rodrigo P, Almeida P (2017) Conflicting effects of climate and vector behavior on the spread of a plant pathogen. Phytobiomes 1:46–53CrossRefGoogle Scholar
  7. Desbiez C, Lecoq H (1997) Zucchini yellow mosaic virus. Plant Pathol 46:809–829CrossRefGoogle Scholar
  8. Desbiez C, Lecoq H (2008) Watermelon mosaic virus and zucchini yellow mosaic virus. In: Mahy BWJ, Van Regenmortel MHV (eds) Encyclopedia of virology. Elsevier, Amsterdam, pp 433–440Google Scholar
  9. Domier LL, Franklin KM, Hunt AG, Rhoads RE, Shaw JG (1989) Infectious in vitro transcripts from cloned cDNA of a potyvirus, tobacco vein mottling virus. Proc Natl Acad Sci U S A 86:3509–3513CrossRefGoogle Scholar
  10. Gal-On A (2007) Zucchini yellow mosaic virus: insect transmission and pathogenicity - the tails of two proteins. Mol Plant Pathol 8:139–150CrossRefGoogle Scholar
  11. Gal-On A, Antignus Y, Rosner A, Raccah B (1991) Infectious in vitro RNA transcripts derived from cloned cDNA of the cucurbit potyvirus, zucchini yellow mosaic virus. J Gen Virol 72:2639–2643CrossRefGoogle Scholar
  12. Gal-On A, Meiri E, Hua WJ, Raccah B, Gaba V (1995) Particle bombardment drastically increases the infectivity of cloned DNA of zucchini yellow mosaic potyvirus. J Gen Virol 76:3223–3227CrossRefGoogle Scholar
  13. Gyeonggi Agricultural Technology Institute (2018) Pest occurrence information 2018 (notice and forecast). In: Lee YS (ed) Gyeonggi Agricultural Technology Institute web, Gyeonggi Agricultural Technology InstituteGoogle Scholar
  14. Hong YH, Chen YK (2011) Zucchini yellow mosaic virus causes begonia chlorotic ringspot in Chinese Taipei. Acta Hortic 901:173–179CrossRefGoogle Scholar
  15. Kang M, Seo JK, Choi H, Choi HS, Kim KH (2016) Establishment of a simple and rapid gene delivery system for cucurbits by using engineered of zucchini yellow mosaic virus. Plant Pathol J 32:70–76CrossRefGoogle Scholar
  16. Katis NI, Tsitsipis JA, Lykouressis DP, Papapanayotou A, Margaritopoulos JT, Kokinis GM, Perdikis DC, Manoussopoulos IN (2006) Transmission of zucchini yellow mosaic virus by colonizing and non-colonizing aphids in Greece and new aphid species vectors of the virus. J Phytopathol 154:293–302CrossRefGoogle Scholar
  17. Kim NY, Lee HJ, Park MR, Hong JS, Jeong RD (2018) First report of infection of Fallopia multiflora with cucumber mosaic virus and zucchini yellow mosaic virus. J Plant Pathol 100:333–333CrossRefGoogle Scholar
  18. KMA (2018) 2017 Report of abnormal climate. In: Kim HK (ed) KMAWeb, Korea Meteorological AdministrationGoogle Scholar
  19. Kwon SW, Kim MS, Choi HS, Kim KH (2005) Biological characteristics and nucleotide sequences of three Korean isolates of zucchini yellow mosaic virus. J Gen Plant Pathol 71:80–85CrossRefGoogle Scholar
  20. Lee KH, Ban YH, Yoon HB, Seo BM, Lee MW, Kim SK, Chang WB, Kim YG (2015) Occurrence of diseases in cucumber in Chungbuk province. Res Plant Dis 21:155–155Google Scholar
  21. Lin SS, Hou RF, Yeh SD (2002) Construction of in vitro and in vivo infectious transcripts of a Taiwan strain of zucchini yellow mosaic virus. Bot Bull Acad Sin 43:261–269Google Scholar
  22. Lisa V, Lecoq H (1984) Zucchini yellow mosaic virus. CMI/AAB Descriptions of Plant Viruses, No. 282, Association of Applied Biologists, Wellesbourne, UKGoogle Scholar
  23. Park DH (2015) High temperature dry weather, viral vector aphids surge. Wonyesanup news paper Web. http://www.wonyesanup.co.kr/news/articleView.html?idxno=30723. Accessed 22 June 2015
  24. Park KS (2016) Spring crops, virus disease alert. Korea Farmers' Daily Newspaper Web. http://newsam.co.kr/news/article.html?no=9094. Accessed 25 April 2016
  25. Park CY, Lee MA, Nam M, Park EH, Bae YS, Lee SH, Kim JS, Won DY (2014) First report of clover yellow vein virus on white clover (Trifolium repens) in South Korea. Plant Dis 98:1450–1450CrossRefGoogle Scholar
  26. Riechmann J, Lain S, Garcia JA (1990) Infectious in vitro transcripts from a plum pox potyvirus cDNA clone. Virology 177:710–716CrossRefGoogle Scholar
  27. Schrijnwerkers CCFM, Huijberts N, Bos L (1991) Zucchini yellow mosaic virus; two outbreaks in the Netherlands and seed transmissibility. Neth J Plant Pathol 97:187–191CrossRefGoogle Scholar
  28. Simmons HE, Holmes EC, Gildow FE, Bothe-Goralczyk MA, Stephenson AG (2011) Experimental verification of seed transmission of zucchini yellow mosaic virus. Plant Dis 95:751–754CrossRefGoogle Scholar
  29. Yamashita T, Lida A, Morikawa H (1991) Evidence that more than 90% of β-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus. Plant Physiol 97:829–831CrossRefGoogle Scholar
  30. Yoon JY, Choi IY, Jang SW, Park SH, Choi SK (2018) First report of zucchini yellow mosaic virus in chayote (Sechium edule) in Korea. Plant Dis 102:1179CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Department of Applied Biology, College of Agriculture and Life SciencesChungnam National UniversityDaejeonSouth Korea
  2. 2.Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal ScienceRural Development AdministrationWanjuSouth Korea
  3. 3.United States Department of Agriculture – Agricultural Research Service, Department of Crop SciencesUniversity of Illinois at Urbana-ChampaignChampaignUSA
  4. 4.United States Department of Agriculture – Agricultural Research ServiceUnited States National Arboretum, Floral and Nursery Plants Research UnitBeltsvilleUSA

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