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Journal of Plant Pathology

, Volume 101, Issue 4, pp 1183–1186 | Cite as

Defensin AFP1 inhibits appressorium formation and penetration of rice cells by the rice blast fungus Magnaporthe oryzae

  • Yoshiyuki SagehashiEmail author
  • Taketo Ashizawa
  • Hiroaki Takaku
  • Osamu Yatou
Short Communication
  • 26 Downloads

Abstract

The plant defensin antifungal peptide 1 (AFP1) is composed of only 51 amino acids and has potent antifungal activity against the rice blast fungus Magnaporthe oryzae. To elucidate the antifungal mechanisms of AFP1, we assessed the suppression of appressorium formation and hyphal penetration by M. oryzae. AFP1 interfered with appressorium formation and host cell penetration following to invasion in a dose-dependent manner. This is the first report of the prevention of M. oryzae infection in rice by AFP1 and our observations could help to develop resistant cultivars and antifungal formulations for use in agriculture.

Keywords

Defensin Antifungal peptide Magnaporthe oryzae Appressorium formation 

Notes

Acknowledgments

We thank Ms. Yumi Ikeda for her technical support in various experiments; Dr. Takayuki Mitsunaga for support with statistical analysis; Dr. Marie Nishimura for kindly providing the M. oryzae strain. M. oryzae Guy11 was used with the special permission of the Ministry of Agriculture, Forestry and Fisheries of Japan.

Compliance with ethical standards

This article does not contain any studies with human participants or animal performed by any of the authors.

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  • Yoshiyuki Sagehashi
    • 1
    Email author
  • Taketo Ashizawa
    • 2
  • Hiroaki Takaku
    • 3
  • Osamu Yatou
    • 4
  1. 1.Hokkaido Agricultural Research CenterNational Agriculture and Food Research OrganizationSapporoJapan
  2. 2.Central Region Agricultural Research CenterNational Agriculture and Food Research OrganizationTsukubaJapan
  3. 3.Department of Applied Life SciencesNiigata University of Pharmacy and Applied Life SciencesAkiha-kuJapan
  4. 4.Hokuriku Research Station, Central Region Agricultural Research CenterNational Agriculture and Food Research OrganizationJoetsuJapan

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