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Journal of Plant Pathology

, Volume 101, Issue 4, pp 1243–1243 | Cite as

First report of celery mosaic virus in Turkey

  • Ali KaranfilEmail author
  • Savas Korkmaz
  • Bayram Cevik
Disease Note
  • 273 Downloads

Keywords

Potyvirus CeMV Detection 

Celery mosaic virus (CeMV), which causes mosaic, vein-clearing, and dwarfing symptoms in celery (Apium graveolens), is a member of the genus Potyvirus in the family Potyviridae (Rose and Maiss 2018). In 2018–2019, symptoms reminiscent of CeMV were seen in celery plants in commercial celery production areas in the Çanakkale province, Turkey. Twenty-one symptomatic celery samples were collected for total nucleic acid extraction by the CTAB method (Li et al. 2008). Two-step RT-PCR was performed using a degenerate potyvirus-specific primer pair (Zheng et al. 2010). As a result, 19 out of 21 collected samples revealed a DNA band of about 350 bp in size, as expected. One of the RT-PCR products, designed as TRCNK, was randomly chosen, cloned into pGEM®-T easy vector system II (Promega, USA), and sequenced. The CeMV sequence was deposited in GenBank with the accession number MK575467. A BlastN analysis of the sequence against the GenBank database resulted in more than 96% identity with other CeMV isolates. To confirm the presence of CeMV, a new primer pair (CeMV_CPF_ 5’-GAGATGGCGGAGGAAGAA-3’ and CeMV_CPR_ 5’-ACTGCATAAACCGAAAGGA-3’) was designed to amplify the complete CeMV coat protein gene by RT-PCR. An amplicon (980 bp) of the expected size was obtained from the same 19 samples that tested positive in RT-PCR with the universal potyvirus primer pair. A randomly chosen RT-PCR product was purified and directly sequenced. The sequence of the complete CP gene (834 bp) was deposited in GenBank with the accession number MK570304. BlastN analysis of the sequence indicated 96–98% identity with other CeMV isolates for which sequence information is available in GenBank. To the best of our knowledge, this is the first report of CeMV in Turkey.

Notes

Acknowledgements

We would like to thank to Dr. Igor Koloniuk for constructive comments on this manuscript.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. Li R, Mock R, Huang Q, Abad J, Hartung J, Kinard G (2008) A reliable and inexpensive method of nucleic acid extraction for the PCR-based detection of diverse plant pathogens. J Virol Methods 154:48–55.  https://doi.org/10.1016/j.jviromet.2008.09.008 CrossRefPubMedGoogle Scholar
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  3. Zheng L, Rodoni BC, Gibbs MJ, Gibbs AJ (2010) A novel pair of universal primers for the detection of potyviruses. Plant Pathol 59:211–220.  https://doi.org/10.1111/j.1365-3059.2009.02201.x CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Department of Plant Protection, Faculty of AgricultureCanakkale Onsekiz Mart UniversityCanakkaleTurkey
  2. 2.Department of Plant Protection, Faculty of Agricultural Sciences and TechnologiesApplied Sciences University of IspartaIspartaTurkey

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