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Integration of anti-penetrant tricyclazole, signaling molecule salicylic acid and root associated Pseudomonas fluorescens enhances suppression of Bipolaris sorokiniana in bread wheat (Triticum aestivum L.)

  • Udai B. SinghEmail author
  • Shailendra Singh
  • Deepti Malviya
  • Nanjappan Karthikeyan
  • Mohd. Imran
  • Rajan Chaurasia
  • Manzar Alam
  • Pratap Singh
  • B. K. Sarma
  • Jai P. Rai
  • T. Damodaran
  • J. K. Tripathi
  • Sunil Kumar
  • Arun K. Sharma
Original Article
  • 12 Downloads

Abstract

Salicylic acid (SA) and root associated Pseudomonas fluorescens are known inducers of systemic resistance and influence plant defense responses including reprogramming of cellular mechanisms in response to biotic stress. However, integration of SA, tricyclazole and bioagent P. fluorescens in the integrated disease management practices is not clearly understood. With this rationale the present study was carried out to understand the effects of integration of an anti-penetrant tricyclazole, signaling molecule SA and P. fluorescens UBSPF-10 in modulation of defense cascades and enhancing suppression of Bipolaris sorokiniana in bread wheat (Triticum aestivum L.). Results showed that exogenous application of SA (100 mg L−1) alone and in combination with tricyclazole (750 mg L−1) and P. fluorescens UBSPF-10 significantly increased activation and accumulation of phenylalanine ammonia lyase (PAL), peroxidase, β 1,3-glucanase and chitinase activities in wheat leaves challenged with B. sorokiniana compared to individually treated and control plants. The influence of combined applications of SA, tricyclazole and P. fluorescens UBSPF-10 on lignin content in plant leaves was investigated and it was observed that the combined treatment resulted significant increase in the acid-soluble lignin content in wheat possibly leading to enhanced suppression of disease progression and severity. Furthermore, correlation analysis clearly indicated that enhanced accumulation of lignin in plant cells significantly reduces pathogen infection and invasion of tissue leading to reduction in disease development compared to other treatments. Therefore, it is concluded that combined application of SA, tricyclazole and P. fluorescens UBSPF-10 played an important role in suppression of B. sorokiniana development in wheat plants in a cooperative manner and thus, could be used in the integrated disease management practices of wheat.

Keywords

Salicylic acid Pseudomonas fluorescens Biotic stress Triticum aestivum Bipolaris sorokiniana Induced systemic resistance 

Notes

Acknowledgments

We gratefully acknowledge to Indian Council of Agricultural Research, New Delhi, India for providing financial support. We express our special thanks to Dr. Anil Kumar Saxena, Director, and staff of ICAR-NBAIM for providing technical support to carry out research work. Authors duly acknowledge the help extended by Sri Mahatam Singh, farmer to carry out the experiment on his own field.

Author contributions

All authors have materially contributed in the research and/or article preparation. All authors have approved the final version of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Statement of human and animal rights

This article does not contain any studies with human or animal subjects performed by the any of the authors.

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  • Udai B. Singh
    • 1
    Email author
  • Shailendra Singh
    • 1
  • Deepti Malviya
    • 1
  • Nanjappan Karthikeyan
    • 1
  • Mohd. Imran
    • 2
  • Rajan Chaurasia
    • 3
  • Manzar Alam
    • 2
  • Pratap Singh
    • 4
  • B. K. Sarma
    • 5
  • Jai P. Rai
    • 5
  • T. Damodaran
    • 6
  • J. K. Tripathi
    • 7
  • Sunil Kumar
    • 1
  • Arun K. Sharma
    • 1
  1. 1.Plant-Microbe Interaction and Rhizosphere Biology LabICAR-National Bureau of Agriculturally Important MicroorganismsMaunath BhanjanIndia
  2. 2.Department of Biosciences, Faculty of ScienceIntegral UniversityLucknowIndia
  3. 3.Department of Botany, Faculty of SciencesBanaras Hindu UniversityVaranasiIndia
  4. 4.Department of Plant Physiology, Institute of Agricultural SciencesBanaras Hindu UniversityVaranasiIndia
  5. 5.Department of Mycology & Plant Pathology, Institute of Agricultural SciencesBanaras Hindu UniversityVaranasiIndia
  6. 6.Regional Research StationICAR-Central Soil Salinity Research InstituteLucknowIndia
  7. 7.ICAR-Indian Institute of Seed SciencesMaunath BhanjanIndia

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