Molecular identification of tomato brown rugose fruit virus in tomato in Palestine
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Tomato, a top cash crop, is infected by a number of viruses that cause drastic yield losses. Recently an unusual viral syndrome that resembled somewhat that induced by tobacco mosaic virus has been observed in Northern Palestine. The most affected tomatoes were of cultivars ‘Ikram’ and ‘Azmeer’. A study aimed at revealing the cause of the disease, identified the presence of an apparently undescribed tobamovirus. The virus genome was entirely sequenced and shown to be composed of 6391 nucleotides. Sequence analysis indicated that this virus was an isolate of tomato brown rugose fruit virus (TBRFV). This is the first time TBRFV was detected in Palestine on tomatoes and the name tomato brown rugose fruit virus-Palestinian isolate (TBRFV-Ps) is suggested. Molecular tools were developed for specific detection of the virus and sanitary actions to protect tomato production from TBRFV were recommended.
KeywordsVirus Tomato Tobamovirus TBRFV Phylogenetic analysis
Authors wish to thank the Science Deanship of An-Najah National University and National Agriculture Research Center for their support of the study. The financial support of An-Najah National University to undertake this work under grant number [ANNU-1718-So029] is also duly acknowledged.
This study was funded by the Science Deanship of An-Najah National University under grant number [ANNU-1718-So029].
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
Corresponding Author has received research grants from An-Najah National University; in collaboration with co-authors from National Agricultural Research Center. Authors declare that they have no conflict of interest.
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