Acidovorax avenae subsp. cattleyae causes bacterial brown spot disease on terrestrial orchid Habenaria lindleyana in Thailand
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Bacterial diseases of orchid are widespread and constitute a serious limiting factor to the production of the terrestrial orchid, Habaneria lindleyana. Bacteria were isolated from terrestrial orchids exhibiting disease symptoms in the Chiang Mai province of Thailand. Initial symptoms were circular, water-soaked leaf spots, which became brown and surrounded by yellow haloes, then enlarged to irregularly-shaped brown blotches followed by necrosis. Pathogenicity tests proved that a bacterium caused the same symptoms on H. lindleyana leaves after inoculation. The bacterium was identified as Acidovorax avenae subsp. cattleyae based on bacteriological analyses and 16S rRNA sequence comparison. Additional pathogenicity tests indicated that an isolate of A. avenae subsp. cattleyae from H. lindleyana could cause brown spots in other orchid genera. To our knowledge, this is the first study conducted to evaluate the importance of A. avenae subsp. cattleyae as an orchid pathogen in the Chiang Mai province of Thailand.
KeywordsOrchid Habaneria Acidovorax Molecular identification
This research was partially supported by the Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Office of Higher Education Commission, Ministry of Education (AG-BIO/PERDO-CHE).
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Conflict of interest
The authors declare that they have no conflict of interest.
Statement of human and animal rights
This article does not contain any studies with human or animal subjects performed by the any of the authors.
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