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Journal of Plant Pathology

, Volume 101, Issue 1, pp 149–153 | Cite as

First report of natural infection of alternanthera yellow vein virus and cotton leaf curl Multan betasatellite on a new host Picrorhiza kurroa, an important endangered medicinal herb

  • Dolly Sharma
  • Aditya Kulshreshtha
  • Rakesh Kumar
  • Vipin HallanEmail author
Short Communication
  • 16 Downloads

Abstract

Picrorhiza kurroa is an ethnopharmacologically important endangered medicinal herb and is traditionally used in Indian Ayurvedic medicine in several preparations. The plant grows as a wild population at an altitude between 3200 and 4500 m. Leaf curl, yellow mosaic and leaf puckering symptoms were observed on P. kurroa grown in a greenhouse at CSIR-IHBT, Palampur, Himachal Pradesh, India. Based on typical symptoms and whitefly infestation, begomovirus infection was suspected. PCR based detection showed the presence of a begomovirus and a betasatellite in all symptomatic plants. The complete sequence analysis of begomovirus and betasatellite characterized their identity as alternanthera yellow vein virus (AlYVV) and cotton leaf curl Multan betasatellite (CLCuMuB), respectively. Further, in phylogeny, both begomovirus and betasatellite were clustered with their respective isolates. This is the first report of a begomovirus and a betasatellite infecting P. kurroa.

Keywords

Picrorhiza kurroa Begomovirus Medicinal herbs Alternanthera yellow vein virus Cotton leaf curl Multan betasatellite 

Notes

Acknowledgements

The authors acknowledge Director, CSIR-IHBT for providing necessary research facilities to carry out the work. Work was done under CSIR network project entitled “Plant Diversity: Studying adaptation biology and understanding / exploiting medicinally important plants for useful bioactives”. Fellowship provided to DS and financial support provided under this project is duly acknowledged. AK is thankful to University Grants Commission (UGC), India, for granting Senior Research Fellowship during tenure of his work. Academy of Scientific and Innovative Research (AcSIR), New Delhi, where AK is registered for Ph.D., is also duly acknowledged.

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018

Authors and Affiliations

  • Dolly Sharma
    • 1
  • Aditya Kulshreshtha
    • 1
    • 2
  • Rakesh Kumar
    • 2
    • 3
  • Vipin Hallan
    • 1
    • 2
    Email author
  1. 1.Plant Virus Lab, Biotechnology DivisionCSIR-IHBTPalampurIndia
  2. 2.Academy of Scientific and Innovative Research (AcSIR)CSIR-Institute of Himalayan Bioresource TechnologyPalampurIndia
  3. 3.Agrotechnology of Medicinal, Aromatic and Commercially Important Plants DivisionCSIR-IHBTPalampurIndia

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