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Biocontrol measures against onion basal rot incidence under natural field conditions

  • Nehal Samy El-Mougy
  • Mokhtar Mohamed Abdel-KaderEmail author
Original Article
  • 16 Downloads

Abstract

Laboratory and field experiments were carried out to evaluate the efficacy of the bio-agents Trichoderma harzianum, T. viride, Bacillus subtilis and Pseudomonas fluorescens and of the fungicide Carbendazim 50WP against onion basal rot caused by Fusarium oxysporum f. sp. cepae. The tested agents were found to be effective in vitro against F. oxysporum f. sp. cepae. The maximum growth inhibition of the fungal pathogen was induced by T. viride (95.5%) and T. harzianum (92.6%) followed by P. fluorescens (88.3%) and B. subtilis (79.3%), respectively. The fungicide Carbendazim 50WP caused a growth reduction of 85.7% at the concentration of 50 ppm. Complete inhibition of pathogen growth (100%) was recorded at 100 ppm. Under field conditions throughout two successive growing seasons, onion transplants immerged in a cell or spore suspension of P. fluorescens and T. viride showed the highest disease reduction, i.e. 69.5 and 61.8%, followed by 53.8, and 53.7% induced by B. subtilis and T. harzianum, respectively. Meanwhile, 22.2% disease reduction over the untreated control was induced by carbendazim 50WP. It can be concluded that the candidate bioagents could be utilized as options alternative to chemical fungicides for the management of basal rot infection of onion, also because they have v advantage of promoting soil fertility.

Keywords

Onion basal rot Biocontrol Carbendazim 50WP Fusarium oxysporum f. sp. cepae 

Notes

Acknowledgements

The authors thank the Administration Board of the Researches and Production Station, National Research Center, Beheira governorate for providing facilities to carry out this work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Statement of human and animal rights

This article does not contain any studies with human or animal subjects performed by the any of the authors.

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Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Plant Pathology DepartmentNational Research CentreGizaEgypt

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