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First report of Geotrichum candidum causing postharvest sour rot of carrot in Punjab, Pakistan

  • Akhtar Hameed
  • Muhammad Waqar AlamEmail author
  • Abdul Rehman
  • Khalid Naveed
  • Muhammad Atiq
  • Nasir Ahmed Rajput
  • Sohaib Sarfraz
  • Nadia Liaqat
  • Faizan Ahmad Tahir
Disease Note
  • 91 Downloads

Keywords

Carrot Geotrichum candidum Pathogenicity 

In December 2017, a severe sour rot of carrot (Daucus carota cv. ‘Kohinoor’) was found on roots of harvested carrots in commercial storage houses of Faisalabad (31°25′15.7620″ N; 73°5′21.4584″ E) - district of Punjab Province. Initially, water-soaked lesions appeared at the surface of carrots that extended deep into the root tissue. At later stages, the surface of diseased lesions was covered with fluffy, white mycelium and produced a sour odor. On potato dextrose agar, sterilized segments of roots displayed white, flat, and nearly powdery colonies, sometimes feathered at the margins. Mycelium was hyaline and septate. Arthroconidia were hyaline, one-celled, and aerial (4.4–12.5 × 4.1–7.2 μm). Cultural and morphological characteristics matched the descriptions of Geotrichum candidum (de Hoog et al. 1986). DNA was extracted from multiple isolates and the Internal Transcribed Spacer (ITS) region was amplified using ITS1/ITS4 primers. The sequences were all identical and two representative sequences were submitted to GenBank (accession Nos. MH729185 and MK215811), which had 99% identity to G. candidum isolate CBS 11628 (KY103456). The culture was deposited in First Culture Bank of Pakistan. Pathogenicity test was confirmed by inoculation of 10 carrot roots with 20 mL of a conidial suspension (105 conidia/mL) using a glass sprayer. Inoculated carrots were placed on a tray and maintained at 20 °C for one week. Ten control carrots were sprayed with sterilized water only. After three days, all inoculated carrots became infected and displayed the same symptoms as seen for naturally infected carrots, and fungus having morphological characters identical to G. candidum was re-isolated. Control carrots remained symptomless. Sour rot of carrot caused by G. candidum has been reported previously in Korea (Kim et al. 2011). To our knowledge, this is the first report of G. candidum associated with sour rot of carrot in Pakistan.

References

  1. de Hoog GS, Smith MT, Gueho E (1986) A revision of the genus Geotrichum and its teleomorphs. Stud Mycol 29:1–131Google Scholar
  2. Kim YK, Kim TS, Shim HS, Park KS, Yeh WH, Hong SJ, Shim CK, Kim JS, Park JO, Han EJ, Lee ML, Jee HJ (2011) First report of sour rot on post-harvest oriental melon, tomato, cucumber, potato, pumpkin and carrot caused by Geotrichum candidum. Research in Plant Disease 17:232–234CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  • Akhtar Hameed
    • 1
  • Muhammad Waqar Alam
    • 2
    Email author
  • Abdul Rehman
    • 1
  • Khalid Naveed
    • 1
  • Muhammad Atiq
    • 1
  • Nasir Ahmed Rajput
    • 1
  • Sohaib Sarfraz
    • 1
  • Nadia Liaqat
    • 1
  • Faizan Ahmad Tahir
    • 1
  1. 1.Department of Plant PathologyUniversity of AgricultureFaisalabadPakistan
  2. 2.Institute of Agricultural SciencesUniversity of the PunjabLahorePakistan

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