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First report of Colletotrichum karstii causing anthracnose on citrus in the Mediterranean region of Turkey

  • Aysun Uysal
  • Şener KurtEmail author
Disease Note
  • 76 Downloads

Keywords

Anthracnose Citrus Colletotrichum Pathogenicity 

In the early 2011, anthracnose-like symptoms were observed on lemon (Citrus lemon L.) cv. ‘Kütdiken’ and mandarin (Citrus reticulata L.) cv. ‘Okitsu’ in Southern Turkey. Symptoms appeared as dieback and wither-tip of twigs, followed by circular light tan areas with purple margins on leaves. In severe attack infections, diseased fruits produced black acervuli on lesions (1–3 mm). Single spore isolations on potato dextrose agar (PDA) formed flat cultures with entire margins, white aerial mycelia and pink conidial masses. Conidia were hyaline, one celled, cylindrical, measuring 11.54–16.0 × 4.0–5.6 μm (n = 40). The isolates were assigned to the C. karstii (Guarnaccia et al. 2017). Fungal DNA was amplified using primers ITS1/ITS4, GDF1/GDR1, ACT-512F/ACT-783R, and CHS-79F/CHS-345R (Damm et al. 2012). These sequences were deposited in GenBank under mandarin isolate MC1 (Accession Nos. MH051303, MG387955, MG951774, and MG951825) and lemon isolate LC7 (Accession Nos. MH028213, MF616515, MG951784, and MG951820) for ITS, ACT, GAPDH, CHS-1, respectively. A BLASTn search of these sequences showed 100% identity to those of Colletotrichum karstiias per Damm et al. 2012.  Pathogenicity was carried out on previously wounded lemon cv. ‘Kütdiken’ leaves and fruits by inoculating with a conidial suspension (1 × 106 conidia per ml) of C. karstii isolates. Control plant organs were treated with sterilized water. All inoculated plants developed symptoms similar to those observed on naturally affected plants, whereas no lesions developed on the control. The pathogen has previously been reported from Asia, Europe, and South Africa (Farr and Rossman 2018). To our knowledge, this is the first report of C. karstii infecting citrus species in Turkey.

References

  1. Damm U, Cannon PF, Woudenberg JHC, Johnston PR, Weir BS, Tan YP, Shivas RG, Crous PW (2012) The Colletotrichum boninense species complex. Stud Mycol 73:1–36CrossRefGoogle Scholar
  2. Farr DF, Rossman AY (2018) Fungal Databases, U.S. National Fungus Collections, ARS, USDA. Retrieved from https://nt.arsgrin.gov/fungaldatabases/. Accessed 16 May 2018
  3. Guarnaccia V, Groenewald JZ, Polizzi G, Crous PW (2017) High species diversity in Colletotrichum associated with citrus diseases in Europe. Persoonia 39:32–50CrossRefGoogle Scholar

Copyright information

© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2019

Authors and Affiliations

  1. 1.Department of Plant Protection, Faculty of Agriculture, and Centre for Implementation and Research of Plant Health ClinicMustafa Kemal UniversityAntakyaTurkey

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