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Bioactive glass/hydroxyapatite- containing electrospun poly (ε-Caprolactone) composite nanofibers for bone tissue engineering

  • Aylin M. Deliormanlı
  • Rabia Konyalı
Research
  • 42 Downloads

Abstract

In this study, bioactive glass and hydroxyapatite (HA)-containing poly(ε-caprolactone) (PCL) nanocomposite fiber mats were fabricated through electrospinning. For this purpose, microscale bioactive glass (silicate-based 45S5 and borate-based 13-93B3 compositions) or HA particles (at 10 wt%) were incorporated into the PCL matrix. The fabricated biocomposite fibers were investigated in terms of morphological and chemical properties. An in vitro mineralization assay in simulated body fluid was performed to understand the capability of the composite electrospun fibers to induce the formation of hydroxycarbonate apatite. Results showed that the diameter of the electrospun PCL-based fibrous scaffolds increased by the inclusion of bioactive glass or HA particles. All of the fibrous mats prepared in the study showed hydrophobic character. Relatively high contact angles (> 90°) obtained for fibrous scaffolds was attributed to the high porosity and surface roughness. Bioactive glass or HA addition to the PCL matrix enhanced the bioactivity of the fibrous scaffolds. The deposition rate of calcium phosphate-based material precipitates was higher on the surface of HA-containing samples compared to bioactive glass-containing PCL scaffolds. Additionally, mineralization ability of borate-based 13-93B3 glass-containing samples was higher compared to 45S5 glass-containing PCL fibers. The biocomposite fibrous scaffolds prepared in the study may find applications in wound healing as wound dressing and in bone tissue engineering.

Keywords

PCL Bioactive glass Nanofibers Electrospinning Tissue engineering 

References

  1. 1.
    Zeimaran, E., Pourshahrestani, S., Djordjevic, I., Pingguan-Murphy, B., Kadri, N.A., Towler, M.R.: Bioactive glass reinforced elastomer composites for skeletal regeneration: a review. Mater Sci Eng C. 53, 175–188 (2015)CrossRefGoogle Scholar
  2. 2.
    Kouhi, M., Morshed, M., Varshosaz, J., Fath, M.H.: Poly (e-caprolactone) incorporated bioactive glass nanoparticles and simvastatin nanocomposite nanofibers: preparation, characterization and in vitro drug release for bone regeneration applications. Chem Eng J. 228, 1057–1065 (2013)CrossRefGoogle Scholar
  3. 3.
    Fabbri, P., Cannillo, V., Sola, A., Dorigato, A., Chiellini, F.: Highly porous polycaprolactone-45S5 Bioglass® scaffolds for bone tissue engineering. Compos Sci Technol. 70(13), 1869–1878 (2010)CrossRefGoogle Scholar
  4. 4.
    Kim, H.W., Knowles, J.C., Kim, H.E.: Effect of biphasic calcium phosphates on drug release and biological and mechanical properties of poly(e-caprolactone) composite membranes. J Biomed Mater Res A. 70, 467–479 (2004)CrossRefGoogle Scholar
  5. 5.
    Gerhardt, L.-C., Boccaccini, A.R.: Bioactive glass and glass-ceramic scaffolds for bone tissue engineering. Materials. 3(7), 3867–3910 (2010)CrossRefGoogle Scholar
  6. 6.
    Rahaman, M.N., Day, D.E., Bal, B.S., Fu, Q., Jung, S.B., Bonewald, L.F., Tomsia, A.P.: Bioactive glass in tissue engineering. Acta Biomater. 7(6), 2355–2373 (2011)CrossRefGoogle Scholar
  7. 7.
    Hench, L.L.: Bioceramics. J Am Ceram Soc. 81, 1705–1728 (1998)CrossRefGoogle Scholar
  8. 8.
    Hench, L.L.: The story of Bioglass®. J Mater Sci Mater Med. 17, 967–978 (2006)CrossRefGoogle Scholar
  9. 9.
    Hench, L.L., Splinter, R.J., Allen, W.C., Greenlee, T.K.: Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res Symp. 334, 117–141 (1971)CrossRefGoogle Scholar
  10. 10.
    Liang, W., Rahaman, M.N., Day, D.E., Marion, N.W., Riley, G.C., Mao, J.J.: Bioactive borate glass scaffold for bone tissue engineering. J Non-Cryst Solids. 354(15–16), 1690–1696 (2008)CrossRefGoogle Scholar
  11. 11.
    Ródenas-Rochina, J., Ribelles, J.L.G., Lebourg, M.: Comparative study of PCL-HAp and PCL-bioglass composite scaffolds for bone tissue engineering. J Mater Sci Mater Med. 24, 1293–1308 (2013)CrossRefGoogle Scholar
  12. 12.
    Cannillo, V., Chiellini, F., Fabbri, P., Sola, A.: Production of Bioglass® 45S5–polycaprolactone composite scaffolds via salt-leaching. Compos Struct. 92, 1823–1832 (2010)CrossRefGoogle Scholar
  13. 13.
    Ji, L., Wang, W., Jin, D., Zhou, S., Song, X.: In vitro bioactivity and mechanical properties of bioactive glass nanoparticles/polycaprolactone composites. Mater Sci Eng C. 46, 1–9 (2015)CrossRefGoogle Scholar
  14. 14.
    Poh, P.S., Hutmacher, D.W., Stevens, M.M., Woodruff, M.A.: Fabrication and in vitro characterization of bioactive glass composite scaffolds for bone regeneration. Biofabrication. 5, 045005 (2013)CrossRefGoogle Scholar
  15. 15.
    Teo, W.-E., Inai, R., Ramakrishna, S.: Technological advances in electrospinning of nanofibers. Sci Technol Adv Mater. 12, 013002 (2011)CrossRefGoogle Scholar
  16. 16.
    Putti, M., Simonet, M., Solberg, R., Peters, G.W.M.: Electrospinning poly(ε-caprolactone) under controlled environmental conditions: influence on fiber morphology and orientation. Polymer. 63, 189–195 (2015)CrossRefGoogle Scholar
  17. 17.
    Otadi, M., Mohebbi-Kalhori, D.: Evaluate of different bioactive glass on mechanical properties of nanocomposites prepared using electrospinning method. Procedia Materials Sci. 11, 196–201 (2015)CrossRefGoogle Scholar
  18. 18.
    Lepry, W.C., Smith, S., Liverani, L., Boccaccini, A.R., Nazhat, S.N.: Acellular bioactivity of sol-gel derived borate glass-polycaprolactone electrospun scaffolds. Biomed Glasses. 2, 88–98 (2016)CrossRefGoogle Scholar
  19. 19.
    Liverani, L., Lacina, J., Roether, J.A., Boccardi, E, Killian, M.S., Schmuki, P., Schubert, D.W., Boccaccini, A.R.: Incorporation of bioactive glass nanoparticles in electrospun PCL/chitosan fibers by using benign solvents, Bioactive Materials. 3, 55-63 (2018) Google Scholar
  20. 20.
    Pirayesh, H., Nychka, J.A.: Sol-gel synthesis of bioactive glass-ceramic 45S5 and its in vitro dissolution and mineralization behavior, J Am Ceram Soc, 96, 1643-1650 (2013)Google Scholar
  21. 21.
    Deliormanlı, A.M.: Synthesis and characterization of Ce+3 and Ga+3 doped borate based bioactive glass scaffolds for bone tissue engineering applications. J Mater Sci Mater Med. 26(2), 67 (2015)CrossRefGoogle Scholar
  22. 22.
    Fu, Q., Rahaman, M.N., Fu, H., Liu, X.: Silicate, borosilicate, and borate bioactive glass scaffolds with controllable degradation rate for bone tissue engineering applications. I. Preparation and in vitro degradation. J Biomed Mater Res A. 95(1), 164–171 (2010)CrossRefGoogle Scholar
  23. 23.
    Turk, M., Deliormanlı, A.M.: Electrically conductive borate-based bioactive glass scaffolds for bone tissue engineering applications. J Biomater Appl. 32(1), 28–39 (2017)CrossRefGoogle Scholar
  24. 24.
    Sultana, N., Khan, T.H.: In vitro degradation of PHBV scaffolds and nHA/PHBV composite scaffolds containing hydroxyapatite nanoparticles for bone tissue engineering. J Nanomater. 2012, Article ID 190950 (2012)Google Scholar
  25. 25.
    Zhu, X., Cui, W., Li, X., Jin, Y.: Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. Biomacromolecules. 9, 1795–1801 (2008)Google Scholar
  26. 26.
    Kokubo, T., Kushitani, H., Saka, S., Kitsugi, T., Yamamuro, T.: Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. J Biomed Mater Res. 24, 721–734 (1990)CrossRefGoogle Scholar
  27. 27.
    Faure, J., Drevet, R., Lemelle, A., Ben Jaber, N., Tara, A., El Btaouri, H., Benhayoune, H.: A new sol–gel synthesis of 45S5 bioactive glass using an organic acid as catalyst. Mater Sci Eng C. 47(1), 407–412 (2015)CrossRefGoogle Scholar
  28. 28.
    Deliormanlı, A.M.: Preparation and in vitro characterization of electrospun 45S5 bioactive glass nanofibers. Ceram Int. 41(1), 417–425 (2015)CrossRefGoogle Scholar
  29. 29.
    Dias, J.R., Antunes, F.E., Bártolo, P.J.: Influence of the rheological behaviour in electrospun PCL nanofibres production for tissue engineering applications. Chem Eng Trans. 32, 1015–1020 (2013)Google Scholar
  30. 30.
    Nezarati, R.M., Eifert, M.B., Cosgriff-Hernandez, E.: Effects of humidity and solution viscosity on electrospun fiber morphology. Tissue Eng Part C Methods. 19(10), 810–819 (2013)CrossRefGoogle Scholar
  31. 31.
    Mohammadkhah, A., Marquardt, L.M., Sakiyama-Elbert, S.E., Day, D.E., Harkins, A.B.: Fabrication and characterization of poly-(ε)-caprolactone and bioactive glass composites for tissue engineering applications. Mater Sci Eng C. 49, 632–639 (2015)CrossRefGoogle Scholar
  32. 32.
    Faghihnejad, A., Zeng, H.: Interaction mechanism between hydrophobic and hydrophilic surfaces: using polystyrene and mica as a model system. Langmuir. 29, 12443–12451 (2013)CrossRefGoogle Scholar
  33. 33.
    Gönen, S.Ö., Erol Taygun, M., Küçükbayrak, S.: Fabrication of bioactive glass containing nanocomposite fiber mats for bone tissue engineering applications. Compos Struct. 138(15), 96–106 (2016)CrossRefGoogle Scholar
  34. 34.
    Notingher, I., Jones, J.R., Verrier, S., Bisson, I., Embanga, P., Edwards, P., Polak, J.M., Hench, L.L.: Application of FTIR and Raman spectroscopy to characterization of bioactive materials and living cells. Spectroscopy. 17, 275–288 (2003)CrossRefGoogle Scholar
  35. 35.
    Sepulveda, P., Jones, J.R., Hench, L.L.: Characterization of melt-derived 45S5 and sol-gel-derived 58S bioactive glasses. J Biomed Mater Res (Appl Biomater). 58, 734–740 (2001)CrossRefGoogle Scholar
  36. 36.
    Yohe, S.T., Freedman, J.D., Falde, E.J., Colson, Y.L., Grinstaff, M.W.: A mechanistic study of wetting Superhydrophobic porous 3D meshes. Adv Funct Mater. 7; 23(29), 3628–3637 (2013)CrossRefGoogle Scholar
  37. 37.
    Meikandan, M., Malarmohan, K.: Fabrication of a superhydrophobic nanofibres by electrospinning. Dig J Nanomater Biostruct. 12(1), 11–17 (2017)Google Scholar
  38. 38.
    Li, S., and Barber, A.H.: Creating superhydrophobic polycarbonate fiber network from hydrophilic polycarbonate through electrospinning. Mater. Res. Soc. Symp. Proc. Vol. 1 © 2012 Materials Research SocietyGoogle Scholar
  39. 39.
    Hassan, M.I., Sultana, N.: Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane, 3 Biotech. 7(4), 249 (2017)Google Scholar
  40. 40.
    Saeed, S.M., Mirzadeh, H., Zandi, M., Barzin, J.: Designing and fabrication of curcumin loaded PCL/PVA multi-layer nanofibrous electrospun structures as active wound dressing. Prog Biomater. 6, 39–48 (2017)CrossRefGoogle Scholar
  41. 41.
    Miguez-Pacheco, V., Hench, L.L., Boccaccini, A.R.: Bioactive glasses beyond bone and teeth: emerging applications in contact with soft tissues. Acta Biomater. 13, 1–15 (2015)CrossRefGoogle Scholar
  42. 42.
    Ranjbar-Mohammadi, M., HajirBahrami, S.: Development of nanofibrous scaffolds containing gum tragacanth/poly (ε-caprolactone) for application as skin scaffolds. Mater Sci Eng C. 48, 71–79 (2015)CrossRefGoogle Scholar
  43. 43.
    Liverani, L., Boccardi, E., Maria Beltrán, A., Boccaccini, A.R.: Incorporation of calcium containing mesoporous (MCM-41-type) particles in electrospun PCL fibers by using benign solvents. Polymers. 9(487), 1–16 (2017)Google Scholar
  44. 44.
    Kister, G., Cassanas, G., Bergounhon, M., Hoarau, D., Vert, M.: Structural characterization and hydrolytic degradation of solid copolymers of d,l-lactide-co-ε-caprolactone by Raman spectroscopy. Polymer. 41(3), 925–932 (2000)CrossRefGoogle Scholar
  45. 45.
    Tas, A.C., Bhaduri, S.B.: Rapid coating of Ti6Al4V at room temperature with a calcium phosphate solution similar to 10× simulated body fluid. J Mater Res. 19(9), 2742–2749 (2004)CrossRefGoogle Scholar
  46. 46.
    Berzina-Cimdina, L., Borodajenko, N.: Research of calcium phosphates using Fourier transform infrared spectroscopy, Infrared spectroscopy—materials science, engineering and technology, Prof. Theophanides Theophile (Ed.). J Mater Sci Mater Med. 123–149 (2012)Google Scholar
  47. 47.
    Huang, W., Day, D.E., Kittiratanapiboon, K., Rahaman, M.N.: Kinetics and mechanisms of the conversion of silicate (45S5), borate, and borosilicate glasses to hydroxyapatite in dilute phosphate solution. J Mater Sci Mater Med. 17, 583–596 (2006)CrossRefGoogle Scholar
  48. 48.
    Liu, X., Rahaman, M.N., Day, D.E.: Conversion of melt-derived microfibrous borate (13-93B3) and silicate (45S5) bioactive glass in a simulated body fluid. J Mater Sci Mater Med. 24(3), 583–595 (2013)CrossRefGoogle Scholar

Copyright information

© Australian Ceramic Society 2018
corrected publication July/2018

Authors and Affiliations

  1. 1.Faculty of Engineering, Department of Metallurgical and Materials EngineeringManisa Celal Bayar UniversityManisaTurkey

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