Journal of the Australian Ceramic Society

, Volume 55, Issue 1, pp 145–155 | Cite as

Preparation and in vitro investigation on bioactivity of magnesia-contained bioactive glasses

  • Sampath Kumar ArepalliEmail author
  • Himanshu Tripathi
  • Partha Pratim Manna
  • Paliwal Pankaj
  • Sairam Krishnamurthy
  • Shashikant C.U. Patne
  • Ram Pyare
  • S. P. SinghEmail author


Mg plays an important role in the human skeletal system as it stimulates the bone formation and reduces bone resorption. Magnesium has been substituted into SiO2–Na2O–CaO–P2O5 bioactive glass. In the present work, the bioactive glasses were designed to enhance bioactivity. Bioactivity of these glasses was assessed by the immersion of the samples in simulated body fluid (SBF) for different time periods. The formation of hydroxy carbonate apatite (HCA) layer was confirmed by scanning electron microscopy, X-ray diffractometry, and FTIR spectrometry which had shown the HCA layer formation and growth. The formation of HCA layer was found to increase on the surface of the SBF immersed bioactive glasses with increasing magnesia contents in the glass. In vitro cell culture investigations such as viability, proliferation, and cell attachment were studied using human osteosarcoma U2-OS cell lines. The in vitro results of new magnesium containing bioactive glasses had shown improved bioactivity as well as better biocompatibility.


Magnesium oxide Bioactive glass Cytocompatibility Cell attachment 



The authors gratefully acknowledge the Head of the Department of Ceramic Engineering, IIT (BHU) and the honorable Director, Indian Institute of Technology (BHU) Varanasi, India for providing the necessary facilities for the present research work. The author, Sampath Kumar Arepalli, is also very much grateful to the University Grants Commission, New Delhi, India (F.14-2(SC)/2010(SA-III)) for providing Rajiv Gandhi National Fellowship for this research work. The authors are thankful to central instrument facility centre (CIFC), IIT(BHU), Varanasi, India for providing the SEM and EDS facilities for this research work.

Funding information

The present work was supported by the grant from the Department of Biotechnology (DBT), New Delhi, India, Nos. BT/PR11490/BRB/10/675/2008 (PPM).


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Copyright information

© Australian Ceramic Society 2018

Authors and Affiliations

  1. 1.Department of Ceramic EngineeringIndian Institute of Technology (Banaras Hindu University)VaranasiIndia
  2. 2.Immunobiology Laboratory, Department of ZoologyBanaras Hindu UniversityVaranasiIndia
  3. 3.Department of PharmaceuticsIndian Institute of Technology (Banaras Hindu University)VaranasiIndia
  4. 4.Department of Pathology, Institute of Medical ScienceBanaras Hindu UniversityVaranasiIndia

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