Journal of Analysis and Testing

, Volume 2, Issue 4, pp 332–341 | Cite as

Size-dependent modulation of CoOOH nanoflakes light scattering for rapid and selective detection of tetracycline in milk

  • Yin Hui Wei
  • Xin Yu Li
  • Jie Gao
  • Jia Jun Liu
  • Dan Yuan
  • Bin Cheng YinEmail author
  • Jian WangEmail author
Original Paper


As an antibiotic, tetracycline has been widely used against bacterial infection or as feed additives for growth promotion, which may lead to the enrichment of antibiotic residues in animals or their products. Therefore, the detection of tetracycline is closely related to human health. The morphological transformation of CoOOH nanoflakes induced by tetracycline was monitored by scanning electron microscope images, dark-field light scattering images and optical spectra, which found that tetracycline could decompose CoOOH nanoflakes into small nanoparticles with low light scattering signal. As a result, the reduced light scattering of CoOOH nanoflakes was proportional to the increased concentration of tetracycline in the range of 1.0–250 μmol/L, and the limit of determination (LOD) was 0.23 μmol/L (3σ/k). Most foreign substances did not interfere in the analysis of tetracycline. Furthermore, the concentration of tetracycline in different milk samples detected with the standard addition method was so low that it accorded with the safety regulation, which was similar to the detection result with high-performance liquid chromatography (HPLC).


CoOOH nanoflakes Light scattering Tetracycline Milk 



This work was supported by the National Natural Science Foundation of China (No. 21405123), the Innovation and Entrepreneurship Project of Southwest University (20172902008) and the College of Pharmaceutical Sciences Innovation and Entrepreneurship Project of Southwest University (YX2017-CXYB-10), as well as the Open Funding Project of the State Key Laboratory of Bioreactor Engineering.

Compliance with Ethical Standards

Conflict of interest

The author(s) declare that they have no competing interests.


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Copyright information

© The Nonferrous Metals Society of China 2018

Authors and Affiliations

  1. 1.Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Ministry of Education), College of Pharmaceutical ScienceSouthwest UniversityChongqingChina
  2. 2.Laboratory of Biosystem and Microanalysis, State Key Laboratory of Bioreactor EngineeringEast China University of Science and TechnologyShanghaiChina

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