Salmonella Enteritidis is a major public health threat of global proportions, while it is still a serious challenge to develop point-of-care detection assay. In this study, nanozymes (Fe-MOF nanoparticles) were successfully synthesized and applied as signal in a colorimetric immunoassay for naked-eye detection of Salmonella Enteritidis. After optimization, the proposed assay was able to detect Salmonella Enteritidis with a detection limit of 34 CFU/mL. The coefficients of variation (CV) of the test were less than 7.0% after 30 days storage at 4 °C. The estimated recoveries in milk samples of the colorimetric immunoassay range from 94.68 to 124%, which indicated the developed method is capable of detecting Salmonella Enteritidis in real samples. This method provides a potential platform for Salmonella detection with naked eyes, which has a significant application value for foodborne pathogen analysis at the point-of-care.
Salmonella Enteritidis Nanozyme Magnetic beads Colorimetric detection Immunoassay
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This work was supported by a WSU start up fund and China National Postdoctoral Program for Innovative Talents (BX20180364).
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