The effect of antimicrobial photodynamic therapy mediated by papain gel on infected dentin in primary teeth: a clinical trial with microbiological evaluation
- 3 Downloads
Antimicrobial photodynamic therapy (aPDT) has been used for treatment of dental caries. Papacarie™ is a gel composed of papain and chloramine employed for the partial removal of carious tissue, effective against bacteria; however, some studies report that this antibacterial action is not quite so evident. The aim of this study is to evaluate the clinical effect of the substance PapaMBlue followed by laser irradiation on infected dentin in dental caries lesion in primary teeth.
Thirty-two primary molars with deep occlusal dental caries were selected and divided in two groups: G1—caries removal with a low-speed drill—and G2—application of aPDT with PapacarieMBlue. After treatment, all the teeth were restored with glass ionomer cement and followed up clinically and radiographically, with evaluations at 3, 6, and 12 months. Dentin samples before and after treatment were analyzed microbiologically.
A reduction in the bacterial count was found in both groups and the inter-group difference was non-significant (p = 0.41022).
It is possible to conclude that there was a microbial reduction with the two treatments proposed; however, PapaMBlue is minimally invasive, maintains healthy tissue integrity, and is easy to use in Pediatric Dentistry and Public Health.
NCT02734420 on 10 March 2016
KeywordsDental caries Antimicrobial photodynamic therapy Low-level laser therapy
The authors gratefully thank FAPESP process number 2016/11711-3 Nove de Julho University for its technological support.
Compliance with ethical standards
This clinical trial received approval from the Human Research Ethics Committee of University Nove de Julho(certificate number: 391563) .
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
Informed consent was obtained from all individual participants included in the study.
- 1.Fejerskov O, Kidd E (2009) Dental caries: the disease and its clinical management. John Wiley & Sons, Blackwell, London.Google Scholar
- 4.Frencken J, Makoni E, Sithole WA Traumatic restorative treatment and glass ionomer cement sealants in school oral health program in Zimbabwe. Evaluation After 1:428–436Google Scholar
- 10.Bijle M, Patil S, Mumkekar SS, Arora N, Bhalla M, Murali K (2013) Awareness of dental surgeons in Pune and Mumbai, India, regarding chemomechanical caries removal system. J Contemp Dent Pract 1(14):1Google Scholar
- 11.Martins MD, Fernandes KPS, Motta LJ, Santos EM, Pavesi VCS, Bussadori SK (2009) Biocompatibility analysis of chemomechanical caries removal material Papacarie on cultured fibroblasts and subcutaneous tissue. J Dent Child 76(2):123–129Google Scholar
- 18.K-i T, Araki K, Mataki S, Kurosaki N (2003) Effects of chloramines and sodium hypochlorite on carious dentin. J Med Dent Sci 50(2):139–146Google Scholar
- 19.Walsh L (1994) Dental lasers: some basic principles. Postgrad Dent 4:26–29Google Scholar
- 20.Miserendino L, Pick RM (1995) Lasers in dentistry. Quintessence Pub Co, Michigan.Google Scholar
- 29.Pupo Y, Gomes GM, Santos EB, Chaves L, Michel MD, Kozlowski V Jr, Gomes O, Gomes JC (2010) Susceptibility of Candida albicans to photodynamic therapy using methylene blue and toluidine blue as photosensitizing dyes. Acta Odontol Latinoam 24(2):188–192Google Scholar