Influence of different surface protocols on dentin bond strength irradiated with Er,Cr:YSGG laser

  • Máyra Andressa R. Valinhos PiccioniEmail author
  • Aline Carvalho Girotto
  • José Roberto Cury Saad
  • Edson Alves de Campos



The aim of this study was to evaluate the influence of Er,Cr:YSGG laser irradiation on the bond strength of different self-etch adhesives systems in normal and artificially hypermineralized dentin.


Two hundred forty healthy bovine incisors were selected. The specimens were randomly divided into two different groups according to the type of dentin. The teeth of each group were randomly divided into five subgroups according to the adhesive system used: Clearfil SE Bond (Kuraray) and Single Bond Universal (3M ESPE). Each subgroup received different treatments: (1) adhesive system (control); (2) acid etching + laser etching + adhesive system; (3) laser etching + acid etching + adhesive system; (4) acid etching + adhesive system; and (5) laser etching + adhesive system. Matrices were positioned, filled with composite resin, and photoactivated for 40 s. After storage period of 24 h in a humid environment, the specimens were submitted to microshear bond strength testing. Subsequently, the fracture pattern of each sample was determined. Four specimens per group were prepared in order to evaluate the interface and/or appearance of resin tags. The data of microshear bond strength (μSBS) were analyzed by two-way ANOVA and Tukey’s (p < 0.05).


Lower μSBS values were found for the (3) and (5) in experiments with both adhesives.


Single Bond Universal self-etching adhesive, after preliminary etching with phosphoric acid, showed highest μSBS values for both substrates in accordance with the manufacturer’s recommendations. The μSBS values were higher for the control treatment using Clearfil SE Bond for both substrates. It may be possible the option of employing the Clearfil SE Bond in dentin sclerotic without the need for complementary etching treatment.


Dentin Self-etch adhesives Laser Microshear bond strength 



Composite resin


Hybrid layer






Compliance with ethical standards

This study was approved by the local Ethics Committee on Animal Research (CEUA) of the School of Dentistry of Araraquara under case number 21/2016 and protocol number 002715.

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Máyra Andressa R. Valinhos Piccioni
    • 1
    Email author
  • Aline Carvalho Girotto
    • 1
  • José Roberto Cury Saad
    • 1
  • Edson Alves de Campos
    • 1
  1. 1.School of Dentistry of AraraquaraUniversidade Estadual Paulista-UNESPAraraquaraBrazil

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