Lasers in Dental Science

, Volume 2, Issue 4, pp 255–263 | Cite as

Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats

  • Paula Delello Macedo
  • Sâmara Tfaile Corbi
  • Fernanda Ali Kitagawa
  • Janice Rodrigues Perussi
  • Anderson Orzari Ribeiro
  • Rosemary Adriana Chierici MarcantonioEmail author
Original Article



The purpose of this study was to evaluate the effectiveness of a photosensitizer hypericin-glucamine, activated by LED amber (34.10 J/cm2) as an adjuvant to scaling and root planing (SRP), on the treatment of experimental periodontal disease (PD) in rats.


In a 15-day period, PD was induced in the mandibular molars through ligature placement. The animals (n = 60) were randomly divided into four groups: control (with induction of periodontal disease), scaling and root planing (SRP), antimicrobial photodynamic therapy (aPDT), and SRP + aPDT. At 7, 15, and 30 days after treatments, animals were euthanized. Digital microtomography, histometric, and stereometric analyses were performed to calculate the bone loss of mandibular second molars, and the tissue repair was analyzed histologically. The data were subjected to statistical analysis (α = 5%).


Histologically, the control group periodontium showed several morphological changes, but an evident gradual reduction in the inflammatory process was observed in the subsequent periods. The SRP, aPDT, and SRP + aPDT groups presented the same, but in less intensity. The stereometric analysis showed a significantly higher proportion of fibroblasts in SRP group (p < 0,001) and SRP + aPDT group (p < 0.0001) compared to that in the control group at 7 days post treatment.


We conclude that scaling and root planing with or without photodynamic therapy with hypericin-glucamine increased density of the fibroblast and cell density. However, there is no statistically significant difference between SRP and SRP + aPDT.


Hypericin-glucamine Scaling and root planing Periodontal disease 



This work was supported in part by a grant from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (BR) (AUXPG 655/2014) and by a grant from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (BR) (133436/2012-8).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Ethical board approval was obtained for this study by the Ethics in Animal Research Committee of the School of Dentistry of Araraquara (UNESP, Brazil CEEA/FOAr 07/2012).


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Paula Delello Macedo
    • 1
  • Sâmara Tfaile Corbi
    • 1
  • Fernanda Ali Kitagawa
    • 1
  • Janice Rodrigues Perussi
    • 2
  • Anderson Orzari Ribeiro
    • 3
  • Rosemary Adriana Chierici Marcantonio
    • 1
    Email author
  1. 1.School of Dentistry Araraquara, Department of Diagnosis and SurgerySão Paulo State University (Unesp)AraraquaraBrazil
  2. 2.Chemistry and Molecular Physics DepartmentUSP, São Paulo UniversitySão CarlosBrazil
  3. 3.Centre for Natural Sciences and HumanitiesUFABC, Federal University of ABCSanto AndréBrazil

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