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Does QoI (strobilurin) resistance in isolates of Puccinia horiana, the causal agent of chrysanthemum white rust, occur in western Japan?

  • Shohei MatsuuraEmail author
Short Communication
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Abstract

Three isolates of chrysanthemum white rust fungus, Puccinia horiana, were collected from two regions in Hiroshima Prefecture, Japan, in 2017. In an in vitro spore germination assay, these isolates showed normal sensitivity to azoxystrobin. In inoculation tests, however, curative spraying of azoxystrobin at the recommended concentration was ineffective at suppressing symptoms caused by two isolates, although preventive spraying suppressed the disease sufficiently. The isolates did not carry the F129L mutation related to resistance to quinone outside inhibitor fungicide in their cytochrome b gene. These results suggest that the sensitivities of P. horiana isolates in Hiroshima to curative application of azoxystrobin may be reduced by mechanisms other than target-site modification.

Keywords

Chrysanthemum white rust Puccinia horiana QoI Strobilurin 

Notes

Acknowledgements

The author thanks Dr. Hideo Ishii of Kibi International University for advice.

Compliance with ethical standards

Conflict of interest

The author declares no conflict of interest.

References

  1. Abiko K, Kishi K, Yoshioka A (1977) Occurrence of oxycarboxin-tolerant isolates of Puccinia horiana P. Hennings in Japan. Ann Phytopath Soc Jpn 43:145–150CrossRefGoogle Scholar
  2. Bartlett DW, Clough JM, Godwin JR, Hall AA, Hamer M, Parr-Dobrzanski B (2002) Strobilurin fungicides. Pest Manag Sci 58:649–662CrossRefPubMedGoogle Scholar
  3. Cook RTA (2001) First report in England of changes in the susceptibility of Puccinia horiana, the cause of chrysanthemum white rust, to triazole and strobilurin fungicides. Plant Pathol 50:792CrossRefGoogle Scholar
  4. Di Rago JP, Coppée JY, Colson AM (1989) Molecular basis for resistance to myxothiazol, mucidin (strobilurin A), and stigmatellin. J Biol Chem 264:14543–14548PubMedGoogle Scholar
  5. Fernández-Ortuño D, Torés JA, de Vicente A, Pérez-García A (2008) Mechanisms of resistance to QoI fungicides in phytopathogenic fungi. Int Microbiol 11:1–9PubMedGoogle Scholar
  6. Fisher N, Brown AC, Sexton G, Cook A, Windass J, Meunier B (2004) Modeling the Qo site of crop pathogens in Saccharomyces cerevisiae cytochrome b. Eur J Biochem 271:2264–2271CrossRefPubMedGoogle Scholar
  7. Grasso V, Palermo S, Sierotzki H, Garibaldi A, Gisi U (2006a) Cytochrome b gene structure and consequences for resistance to Qo inhibitor fungicides in plant pathogens. Pest Manag Sci 62:465–472CrossRefPubMedGoogle Scholar
  8. Grasso V, Sierotzki H, Garibaldi A, Gisi U (2006b) Characterization of the cytochrome b gene fragment of Puccinia species responsible for the binding site of QoI fungicides. Pestic Biochem Physiol 84:72–82CrossRefGoogle Scholar
  9. Ishii H (2006) Impact of fungicide resistance in plant pathogens on crop disease control and agricultural environment. Jpn Agric Res Q 40:205–211CrossRefGoogle Scholar
  10. Kim YS, Dixon EW, Vincelli P, Farman ML (2003) Field resistance to strobilurin (QoI) fungicides in Pyricularia grisea caused by mutations in the mitochondrial cytochrome b gene. Phytopathology 93:891–900CrossRefPubMedGoogle Scholar
  11. Klosowski AC, May De Mio LL, Miessner S, Rodrigues R, Stammler G (2016) Detection of the F129L mutation in the cytochrome b gene in Phakopsora pachyrhizi. Pest Manag Sci 72:1211–1215CrossRefPubMedGoogle Scholar
  12. Palmer CL, Bonde MR, Nester SE, Revell JM, Luster DG (2015) Fungicide impact on in vitro germination of basidiospores of Puccinia horiana, the causal agent of chrysanthemum white rust. Plant Health Prog 16:73–76CrossRefGoogle Scholar
  13. Sugimura T, Nishizaki M (2001) Relation between evaluation of effectiveness of fungicides using pieces of leaf and their control effect to chrysanthemum white rust (Puccinia horiana). Bull Nara Agric Exp Stn 32:40–41 (in Japanese) Google Scholar
  14. Wojdyla AT (2004) Development of Puccinia horiana on chrysanthemum leaves in relation to chemical compounds and time of their application. J Plant Prot Res 44:91–102Google Scholar
  15. Wong FP, Wilcox WF (2001) Comparative physical modes of action of azoxystrobin, mancozeb, and metalaxyl against Plasmopora viticola (grapevine downy mildew). Plant Dis 85:649–656CrossRefPubMedGoogle Scholar
  16. Wood PM, Hollomon DW (2003) A critical evaluation of the role of alternative oxidase in the performance of strobilurin and related fungicides acting at the Qo site of Complex III. Pest Manag Sci 59:499–511CrossRefPubMedGoogle Scholar

Copyright information

© Deutsche Phytomedizinische Gesellschaft 2019

Authors and Affiliations

  1. 1.Hiroshima Prefectural Technology Research Institute, Agriculture Research CenterHigashi-HiroshimaJapan

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