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Journal of Plant Diseases and Protection

, Volume 126, Issue 2, pp 115–127 | Cite as

Volatiles of several grapevine cultivars emitted at different phenological stages linked to discriminatory ability of grapevine moths

  • Margit Rid
  • Anna Markheiser
  • Svenja Stein
  • Christoph Hoffmann
  • Jürgen GrossEmail author
Original Article
  • 50 Downloads

Abstract

The tortricid moths Lobesia botrana (Denis and Schiffermueller) and Eupoecilia ambiguella (Hübner) are major insect pests of grapevine Vitis vinifera. We compared the pattern of volatile organic compounds in headspace samples of four different grapevine cultivars in dependency of their phenological stage by thermodesorption coupled with gas chromatography (GC) and mass spectrometry. Further, we assessed the detection of compounds by the antenna of both moth species by GC coupled with electroantennographical detection. We detected significant differences between the chemical compositions of headspace samples of the four cultivars based on the odor bouquet. By comparing the principal component linear discriminant analysis pattern of only those chemical compounds that could be actually detected by the moths’ antenna, the different cultivars showed similar odor patterns indicating that differences measured by GC may not be detected by the moths’ antenna. The supposed reduced discriminatory ability of both moth species was supported by behavioral bioassays using a Y-tube olfactometer. In connection to the recently published impact of the berries’ visual appearance (shape and color) and nonvolatile compounds of the wax layer, the presented results suggest that reported moth preferences for specific cultivars are not encoded by volatiles only. The results of this study will contribute to the development of an innovative egg-monitoring tool for grapevine moths.

Keywords

Lobesia botrana Eupoecilia ambiguella Vitis vinifera Olfactometer bioassay GC–MS Pattern analysis 

Notes

Acknowledgements

We are grateful to the vine-growers Sven and Walter Stein (Hirschberg, Germany) participating in the project for allowing us to carry out experiments in their vineyard. We are grateful to Sandra Biancu (JKI Siebeldingen, Germany), Sebastian Faus, Sandra Schubach and Kai Lukat (JKI, Dossenheim, Germany) for insect rearing and technical assistance, to Omer Nevo (University of Ulm, Germany) for introduction in AMDIS and to Jannicke Gallinger (JKI Dossenheim) for statistical advices.

Funding

MR and AM were supported by funds of the Federal Ministry of Food and Agriculture (BMEL) based on a decision of the Parliament of the Federal Republic of Germany via the Federal Office for Agriculture and Food (BLE) under the innovation support program number 2814701611.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

41348_2019_214_MOESM1_ESM.pdf (485 kb)
Supplementary material 1 (PDF 484 kb)

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Copyright information

© Deutsche Phytomedizinische Gesellschaft 2019

Authors and Affiliations

  1. 1.Laboratory of Applied Chemical Ecology, Institute for Plant Protection in Fruit Crops and ViticultureJulius Kühn-Institut, Federal Research Centre for Cultivated PlantsDossenheimGermany
  2. 2.Laboratory of Zoology, Institute for Plant Protection in Fruit Crops and ViticultureJulius Kühn-Institut, Federal Research Centre for Cultivated PlantsSiebeldingenGermany
  3. 3.Institute of Evolutionary Ecology and Conservation GenomicsUniversity of UlmUlmGermany
  4. 4.Plant Chemical EcologyTechnical University of DarmstadtDarmstadtGermany

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