Incidence, distribution and limited genetic variability among Turkish isolates of Grapevine Pinot gris virus from different grapevine cultivars
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Grapevine Pinot gris virus (GPGV) was firstly identified in northern Italy by deep sequencing from grapevine cv. Pinot gris, exhibiting mottling and deformation of the leaves. The objective of this study was to investigate the prevalence and genetic variability of GPGV isolates obtained from different local and imported grapevine cultivars in Turkey based on partial coat protein, movement protein and RNA-dependent RNA polymerase (RdRp) domain of the replicase (Rep) gene. Two hundred and one grapevine samples from different provinces were tested by RT-PCR assays, approximately 25% of which were found to be infected by GPGV. The PCR products were sequenced and based on the phylogenetic analysis, RdRp gene was found to be most conserved region. The phylograms of three genomic regions revealed correlation between geography and genetic structure. Furthermore, nucleotide diversity studies revealed a low divergence from the homologous sequences from GenBank and some variations within the groups were detected. The results presented in this study provide a better understanding of genetic variation and phylogenetic of GPGV isolates worldwide.
KeywordsVitis vinifera L. GPGV CP MP Rep Genetic variation
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Conflict of interest
The authors declare that they have no conflicts of interest in the research.
- Casati P, Maghradze D, Quaglino F, Bianco PA (2015) First report of grapevine Pinot gris virus in Georgia. J Plant Pathol 97:S67–S77Google Scholar
- d’Urso F, Ayllon MA, Rubio L, Sambade A, Hermoso de Mendoz A, Guerri J, Moreno P (2000) Contribution of uneven distribution of genomic RNA variants of Citrus tristeza virus (CTV) within the plant to changes in the viral population following aphid transmission. Plant Pathol 49:288–294CrossRefGoogle Scholar
- Malossini U, Moscon R, Ferrazza M, Bianchedi P, Varner M, Credi R (2012) Caratteristichevegeto-produttive div iti pinot grigio e Tramineraromaticoaffette da unanuovavirosisegnalata in Trentino. IV Convegno Nazionale di Viticoltura Conavi. To Asti, 10-11-12 Iuglio, p 37Google Scholar
- Martelli GP (2014) Directory of virus and virus-like diseases of the grapevine and their agents. J Plant Pathol 96:105–120Google Scholar
- Morelli M, de Moraes CA, Susca L, Saldarelli P, Gualandri V, Martelli GP (2014) First report of grapevine Pinot gris virus from table grapes in Southern Italy. J Plant Pathol 96:439Google Scholar