Journal of Plant Diseases and Protection

, Volume 125, Issue 5, pp 469–476 | Cite as

Incidence, distribution and limited genetic variability among Turkish isolates of Grapevine Pinot gris virus from different grapevine cultivars

  • Eminur ElçiEmail author
  • Mona Gazel
  • Vahid Roumi
  • Kadriye Çağlayan
Original Article


Grapevine Pinot gris virus (GPGV) was firstly identified in northern Italy by deep sequencing from grapevine cv. Pinot gris, exhibiting mottling and deformation of the leaves. The objective of this study was to investigate the prevalence and genetic variability of GPGV isolates obtained from different local and imported grapevine cultivars in Turkey based on partial coat protein, movement protein and RNA-dependent RNA polymerase (RdRp) domain of the replicase (Rep) gene. Two hundred and one grapevine samples from different provinces were tested by RT-PCR assays, approximately 25% of which were found to be infected by GPGV. The PCR products were sequenced and based on the phylogenetic analysis, RdRp gene was found to be most conserved region. The phylograms of three genomic regions revealed correlation between geography and genetic structure. Furthermore, nucleotide diversity studies revealed a low divergence from the homologous sequences from GenBank and some variations within the groups were detected. The results presented in this study provide a better understanding of genetic variation and phylogenetic of GPGV isolates worldwide.


Vitis vinifera L. GPGV CP MP Rep Genetic variation 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest in the research.


  1. Al Rwahnih M, Golino D, Rowhani A (2016) First report of grapevine Pinot gris virus infecting grapevine in the United States. Plant Dis 100:1030CrossRefGoogle Scholar
  2. Angelini E, Bertazzon N, Montgomery J, Wang X, Zinkl A, Stamp J, Wei A (2016) Occurrence of grapevine Pinot gris virus in commercial vineyards in the United States. Plant Dis 100:1254CrossRefGoogle Scholar
  3. Bertazzon N, Filippin L, Forte V, Angelini E (2015) Grapevine Pinot gris virus seems to have recently been introduced to vineyards in Veneto, Italy. Arch Virol 161:711–714CrossRefPubMedGoogle Scholar
  4. Bertazzon N, Forte V, Filippin L, Causin R, Maixner M, Angelini E (2017) Association between genetic variability and titre of grapevine Pinot gris virus with disease symptoms. Plant Pathol 66:949–959CrossRefGoogle Scholar
  5. Beuve M, Candresse T, Tannieres M, Lemaire O (2015) First report of grapevine Pinot gris virus (GPGV) in grapevine in France. Plant Dis 99(2):293CrossRefGoogle Scholar
  6. Casati P, Maghradze D, Quaglino F, Bianco PA (2015) First report of grapevine Pinot gris virus in Georgia. J Plant Pathol 97:S67–S77Google Scholar
  7. Cho IS, Jung SM, Cho JD, Choi GS, Lim HS (2013) First report of grapevine Pinot gris virus infecting grapevine in Korea. New Dis Rep 27:10CrossRefGoogle Scholar
  8. d’Urso F, Ayllon MA, Rubio L, Sambade A, Hermoso de Mendoz A, Guerri J, Moreno P (2000) Contribution of uneven distribution of genomic RNA variants of Citrus tristeza virus (CTV) within the plant to changes in the viral population following aphid transmission. Plant Pathol 49:288–294CrossRefGoogle Scholar
  9. Fan XD, Dong YF, Zhang ZP, Ren F, Hu GH, Li ZN, Zhou J (2015) First report of grapevine Pinot gris virus in grapevines in China. Plant Dis 100:540CrossRefGoogle Scholar
  10. Gazel M, Caglayan K, Elçi E, Öztürk L (2016) First report of grapevine Pinot gris virus in grapevine in Turkey. Plant Dis 100:657CrossRefGoogle Scholar
  11. Giampetruzzi A, Roumi V, Roberto R, Malossini U, Yosuhikawa N, La Notte P, Terlizzi F, Credi R, Saldarelli P (2012) A new grapevine virus discovered by deep sequencing of virus- and viroid-derive small RNAs in cv. Pinos gris. Virus Res 163:262–268CrossRefGoogle Scholar
  12. Glasa M, Predajna L, Kominek P, Nagyova A, Candresse T (2014) Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines. Arch Virol 159:2103–2107CrossRefPubMedGoogle Scholar
  13. Jo Y, Choi H, Kyong Cho J, Yoon J-Y, Choi S-K, Kyong Cho W (2015) In silico approach to reveal viral populations in grapevine cultivar Tannat using transcriptome data. Sci Rep 5:15841CrossRefPubMedPubMedCentralGoogle Scholar
  14. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  15. Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452CrossRefGoogle Scholar
  16. MacKenzie DJ, McLean MA, Mukerji S, Green M (1997) Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis 81:222–226CrossRefGoogle Scholar
  17. Maliogka VI, Martelli GP, Fuchs M, Katis NI (2015) Control of viruses infecting grapevine. Adv Virus Res 91:175–227CrossRefPubMedGoogle Scholar
  18. Malossini U, Moscon R, Ferrazza M, Bianchedi P, Varner M, Credi R (2012) Caratteristichevegeto-produttive div iti pinot grigio e Tramineraromaticoaffette da unanuovavirosisegnalata in Trentino. IV Convegno Nazionale di Viticoltura Conavi. To Asti, 10-11-12 Iuglio, p 37Google Scholar
  19. Martelli GP (2014) Directory of virus and virus-like diseases of the grapevine and their agents. J Plant Pathol 96:105–120Google Scholar
  20. Morelli M, de Moraes CA, Susca L, Saldarelli P, Gualandri V, Martelli GP (2014) First report of grapevine Pinot gris virus from table grapes in Southern Italy. J Plant Pathol 96:439Google Scholar
  21. Moya A, Holmes EC, Gonzalez-Candelas F (2004) The population genetics and evolutionary epidemiology of RNA viruses. Nat Rev Microbiol 2:279–288CrossRefPubMedGoogle Scholar
  22. Pérez-Losada M, Porter M, Crandall KA (2008) Methods for analyzing viral evolution. In: Roossinck MJ (ed) Plant virus evolution. Springer, Berlin, pp 165–204CrossRefGoogle Scholar
  23. Plesko PI, Virscek MM, Seljak G, Zezlina I (2014) First report of grapevine Pinot gris virus infecting grapevine in Slovenia. Plant Dis 98:1014CrossRefGoogle Scholar
  24. Rubio L, Guerri J, Moreno P (2013) Genetic variability and evolutionary dynamics of viruses of the family Closteroviridae. Front Microbiol 4:151CrossRefPubMedPubMedCentralGoogle Scholar
  25. Saldarelli P, Giampetruzzi A, Morelli M, Malossini U, Pirolo C, Bianchedi P, Gualandri V (2015) Genetic variability of grapevine Pinot gris virus and its association with grapevine leaf mottling and deformation. Phytopathology 105:555–563CrossRefPubMedGoogle Scholar
  26. Viver MA, Pretioru IS (2002) Genetically tailored grapevines for the wine industry. Trends Biotechnol 20:472–478CrossRefGoogle Scholar
  27. Vives MC, Rubio L, Galipienso L, Navarro L, Moreno P, Guerri J (2002) Low genetic variation between isolates of Citrus leaf blotch virus from different host species and of different geographic origins. J Gen Virol 83:2587–2591CrossRefPubMedGoogle Scholar
  28. Xiao H, Shabanian M, McFadden-Smith W, Meng B (2016) First report of grapevine Pinot gris virus in commercial grapevines in Canada. Plant Dis 100:1030CrossRefGoogle Scholar

Copyright information

© Deutsche Phytomedizinische Gesellschaft 2018

Authors and Affiliations

  • Eminur Elçi
    • 1
    Email author
  • Mona Gazel
    • 2
  • Vahid Roumi
    • 3
  • Kadriye Çağlayan
    • 2
  1. 1.Plant Production and Technologies Department, Faculty of Agricultural Sciences and TechnologiesNiğde Ömer Halisdemir UniversityNiğdeTurkey
  2. 2.Plant Protection Department, Agriculture FacultyMustafa Kemal UniversityHatayTurkey
  3. 3.Plant Protection Department, Faculty of AgricultureUniversity of MaraghehMaraghehIran

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