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Identification and Molecular Characterization of Hc-Upsalin, a Novel Matrix Protein Involved in Nacreous-Layer Biomineralization in Hyriopsis cumingii

  • Xiao-Jun Liu
  • Can Jin
  • Wei Guo
  • Jia-Le LiEmail author
Article
  • 29 Downloads

Abstract

Biomacromolecules, including matrix proteins, are important in shell-mineralization processes, such as inducing crystal nucleation, regulating crystal polymorphism and morphology. In this study, we identified the novel matrix protein hc-upsalin from the freshwater pearl mussel Hyriopsis cumingii, associated with its significantly high similarity to the known matrix protein upsalin in the freshwater mussel Unio pictorum. The full-length cDNA of hc-upsalin encodes 119 amino acids, including a potential signal peptide of 17 residues. The mature protein is characterized by high proportions of Gly (13.7%), Cys (11.8%), and Pro (11.8%), with a molecular weight of 11.4 kDa and a theoretical pI of 6.93. Hc-upsalin is specifically expressed in muscular tissues mostly in the mantle, with positive signals detected in dorsal epithelial cells of the mantle by in situ hybridization, and decreased expression of hc-upsalin leaded to random accumulation of tablet, suggesting its involvement in shell nacreous-layer biomineralization. Furthermore, hc-upsalin gene-expression patterns during pearl biomineralization indicated a potential role in the process of pearl nacreous-layer calcification.

Keywords

Biomineralization Matrix protein Pearl Nacreous layer Hyriopsis cumingii 

Notes

Acknowledgments

This work was financially supported by the National Science and Technology Support Program (2012BAD26B04), the National Natural Science Foundation of China (31272654), and Modern agricultural industrial technology system (CARS-49).

Compliance with Ethical Standards

Conflict of Interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

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© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of AgricultureShanghaiChina
  2. 2.National Demonstration Center for Experimental Fisheries Science EducationShanghai Ocean UniversityShanghaiChina
  3. 3.Shanghai Engineering Research Center of AquacultureShanghaiChina

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