Calmodulin Highly Expressed during the Formation of Pearl Sac in Freshwater Pearl Mussel (Hyriopsis schlegelii)
- 92 Downloads
Calmodulin (CaM) is a pivotal multifunction regulator of calcium metabolism in nearly all organisms, and earlier studies revealed its underlying involvement of shell or pearl formation in oysters. Culture pearls are formed within complete pearl sacs. However, whether CaM functions in pearl sac formation or not is not reported. In this study, we prepared a polyclonal antibody against the CaM from the freshwater pearl mussel Hyriopsis schlegelii (HsCaM) and then detected the expression and distribution of HsCaM in pearl sac using the method of immunofluorescence in situ hybridization (IISH). Results revealed that strong fluorescent signals were observed in pearl sac as well as epithelial border of mantle. In addition, quantitative analysis results indicated that the mRNA expression level of HsCaM in pearl sac of H. schlegelii was significantly up-regulated during pearl sac formation. The fact that HsCaM highly expressed is present in pearl sac suggested that it might facilitate pearl sac formation in H. schlegelii.
KeywordsPearl mussel Pearl sac CaM Expression distribution Development
This work was supported by the Natural Science Foundation of Jiangxi Province, China (20151BAB204035)(To KP); the Key Scientific and Technological Program of Jiangxi Province, China (20121BBF60036)(To KP); the Youth Fund of the Education Department of Jiangxi Province, China(GJJ14219)(To KP); the Science and Technology Landing Project of Jiangxi Province, China (KJLD12001)(To YH); and the National Natural Science Foundation of China (31660337)(To YH).
Compliance with Ethical Standards
Conflict of Interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
- Arnaud-Haond S, Goyard E, Vonau V, Herbaut C, Prou J, Saulnier D (2007) Pearl formation: persistence of the graft during the entire process of biomineralization. Mar Biol 9(1):113–116Google Scholar
- Inoue N, Ishibashi R, Ishikawa T, Atsumi T, Aoki H, Komaru A (2011a) Gene expression patterns in the outer mantle epithelial cells associated with pearl sac formation. Marine. Biotechnology 13(3):474–483Google Scholar
- Inoue N, Ishibashi R, Ishikawa T, Atsumi T, Aoki H, Komaru A (2011b) Can the quality of pearls from the Japanese pearl oyster (Pinctada fucata) be explained by the gene expression patterns of the major shell matrix proteins in the pearl sac? Marine. Biotechnology 13(1):48–55Google Scholar
- Kinoshita S, Wang N, Inoue H, Maeyama K, Okamoto K, Nagai K, Kondo H, Hirono I, Asakawa S, Watabe (2011) Deep sequencing of ESTs from nacreous and prismatic layer producing tissues and a screen for novel shell formation-related genes in the pearl oyster. PLoS One 6(6): e21238Google Scholar
- Peng K, Wang CY, Wang JH, Sheng JQ, Shi JW, Li J, Hong YJ (2014) Molecular cloning, sequence analysis, and cadmium stress-rated expression changes of BTG1 in freshwater pearl mussel, Hyriopsis schlegelii. Zool Res 35(5):389–397Google Scholar
- Peng K, Hu B, Zhao D, Wang J, Sheng J, Wu D, Shi J, Hong YJ (2016) Molecular characterization and expression analysis of Cathepsin L1-like from Hyriopsis schlegelii. J Fish China 40(10):1556–1566 in ChineseGoogle Scholar
- Ren G, Hu X, Tang J, Wang Y (2013) Characterization of cDNAs for calmodulin and calmodulin-like protein in the freshwater mussel Hyriopsis cumingii: differential expression in response to environmental Ca(2+) and calcium binding of recombinant proteins. Comp Biochem Physiol B Biochem Mol Biol 165(3):165–171CrossRefGoogle Scholar