Topics in Current Chemistry

, 378:13 | Cite as

DNA–Iron Oxide Nanoparticles Conjugates: Functional Magnetic Nanoplatforms in Biomedical Applications

  • José Raúl Sosa-Acosta
  • Claudia Iriarte-Mesa
  • Greter A. Ortega
  • Alicia M. Díaz-GarcíaEmail author
Part of the following topical collections:
  1. Surface-modified Nanobiomaterials for Electrochemical and Biomedicine Applications


The use of magnetic nanoparticles (MNPs), such as iron oxide nanoparticles (IONPs), in biomedicine is considered to be a valuable alternative to the more traditional materials due to their chemical stability, cost-effectiveness, surface functionalization, and the possibility to selectively attach and transport targeted species to the desired location under a magnetic field. One of the many main applications of MNPs is DNA separation, which enables genetic material manipulation; consequently, MNPs are used in numerous biotechnological methods, such as gene transfection and molecular recognition systems. In addition, the interaction between the surfaces of MNPs and DNA molecules and the magnetic nature of the resulting composite have facilitated the development of safe and effective gene delivery vectors to treat significant diseases, such as cancer and neurological disorders. Furthermore, the special recognition properties of nucleic acids based on the binding capacity of DNA and the magnetic behavior of the nanoparticles allowing magnetic separation and concentration of analytes have led to the development of biosensors and diagnostic assays; however, both of these applications face important challenges in terms of the improvement of selective nanocarriers and biosensing capacity. In this review, we discuss some aspects of the properties and surface functionalization of MNPs, the interactions between DNA and IONPs, the preparation of DNA nanoplatforms and their biotechnological applications, such as the magnetic separation of DNA, magnetofection, preparation of DNA vaccines, and molecular recognition tools.


Magnetic nanoparticles DNA conjugation Nucleic acid separation DNA-based therapeutics 



(3-Aminopropyl) triethoxysilane


Cu(I)-catalyzed azide-alkyne cycloaddition


Iron oxide nanoparticles


Magnetic nanoparticles




Polyamidoamine dendrimers


Polyethylene glycol




Poly(2-hydroxyethyl methacrylate)


4-Pyridyldithiol-derivatized peptide nucleic acid


Small interfering RNAs


Single-stranded DNA


Single-stranded oligonucleotide


Tetraethyl orthosilane


Tetramethyl orthosilane


Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  1. 1.Laboratory of Bioinorganic (LBI), Department of Inorganic and General Chemistry, Faculty of ChemistryUniversity of HavanaHavanaCuba
  2. 2.Faculty of Chemistry and PharmacyPontifical Catholic University of ChileSantiagoChile
  3. 3.Legaria Unit, Center for Applied Science and Advanced Technology of IPNMexico CityMexico

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