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APPJ Discharge Effects on Human Factor VIII:Ag Level in Healthy and Hemophilia A Patient

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Abstract

Many studies in the field of cold plasma and its application in medicine and biology have been done recently. Some of them reported that cold atmospheric pressure plasma jet (APPJ) discharge accelerates blood clotting because factor VIII (FVIII) is a plasma glycoprotein that may play a role in the coagulation cascade. In this study, we aim to assess the effect of APPJ discharge on factor VIII antigen (FVIII:Ag) in healthy and hemophilic patients. Therefore, the variations of FVIII:Ag in healthy plasma samples and hemophilic plasma samples at the times of 0, 4, 8 and 16 s treatment with APPJ were measured by ELISA technique. Additionally, activated partial thromboplastin time (APTT) test was used to check the blood coagulation rate at different treatment times. Our findings showed that the amount of FVIII:Ag in healthy samples was high to low in seconds 4 > 8 > 16 > 0. The APTT results showed that APPJ increases the blood clot formation, but in hemophilic plasma samples, APPJ reduces the clot formation speed and FVIII:Ag concentration. Accordingly, the APPJ discharge elicited a slight increase in FVIII:Ag concentration in short-term treatment, which could be involved in blood coagulation. Moreover, the effects of APPJ discharge on other coagulation factors need further studies.

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References

  1. Bach J, Haubelt H, Seyfert U, Vogt A, Hoerdt P, Hellstern P (2007) The impact of freezing of plasma samples, ABO blood group and acute-phase reaction on detecting mild factor VIII deficiency and increased factor VIII levels as a risk factor for venous thromboembolism. In: Scharrer I, Schramm W (eds) 36th Hemophilia symposium Hamburg 2005. Springer, Berlin, pp 305–309

  2. Battino M, Bullon P, Wilson M, Newman H (1999) Oxidative injury and inflammatory periodontal diseases: the challenge of anti-oxidants to free radicals and reactive oxygen species. Crit Rev Oral Biol Med 10:458–476

  3. Butenas S, Parhami-Seren B, Undas A, Fass DN, Mann KG (2010) The normal factor VIII concentration in plasma. Thromb Res 126(2):119–123

  4. Choi J, Mohamed AA, Kang SK, Woo KC, Kim KT, Lee JK (2014) 900-MHz nonthermal atmospheric pressure plasma jet for biomedical applications. Plasma Process Polym 27:258–263

  5. Dobrynin D, Wasko K, Friedman G, Fridman AA, Fridman G (2011) Fast blood coagulation of capillary vessels by cold plasma: a rat ear bleeding model. Plasma Med 1:241–247

  6. Fridman G, Peddinghaus M, Balasubramanian M, Ayan H, Fridman A, Gutsol A et al (2006) Blood coagulation and living tissue sterilization by floating-electrode dielectric barrier discharge in air. Plasma Chem Plasma Process 26:425–442

  7. Fridman G, Friedman G, Gutsol A, Shekhter AB, Vasilets VN, Fridman A (2008) Applied plasma medicine. Plasma Process Polym 5:503–533

  8. Heslin C, Boehm D, Milosavljevic V, Laycock M, Cullen PJ, Bourke P (2014) Quantitative assessment of blood coagulation by cold atmospheric plasma. Plasma Med 4:153–163

  9. Hosseinzadeh Colagar A, Sohbatzadeh F, Mirzanejhad S, Omran AV (2010) Sterilization of Streptococcus pyogenes by afterglow dielectric barrier discharge using O2 and CO2 working gases. Biochem Eng J 51:189–193

  10. Hosseinzadeh Colagar A, Memariani H, Sohbatzadeh F, Omran AV (2013) Nonthermal atmospheric argon plasma jet effects on Escherichia coli biomacromolecules. Appl Biochem Biotechnol 171:1617–1629

  11. Hosseinzadeh Colagar A, Alavi O, Sohbatzadeh F (2017a) Lipid peroxidation of Escherichia coli by triplet non-thermal atmospheric pressure plasma jet. Chiang Mai J Sci 42:678–687

  12. Hosseinzadeh Colagar A, Mortazavi SM, Arab-Yarmohammadi V, Sohbatzadeh F (2017b) Molecular effects of atmospheric pressure plasma jet on the double-stranded DNA. Iran J Med Phys 14:29–37

  13. Kalghatgi SU, Fridman G, Cooper M, Nagaraj G, Peddinghaus M, Balasubramanian M et al (2007) Mechanism of blood coagulation by nonthermal atmospheric pressure dielectric barrier discharge plasma. IEEE Trans Plasma Sci 35:1559–1566

  14. Kim GC, Kim GJ, Park SR, Jeon SM, Seo HJ, Iza F et al (2008) Air plasma coupled with antibody-conjugated nanoparticles: a new weapon against cancer. J Phys D, Appl Phys 42:032005

  15. Lacroix-Desmazes S, Wootla B, Dasgupta S, Delignat S, Bayry J, Reinbolt J et al (2006) Catalytic IgG from patients with hemophilia A inactivate therapeutic factor VIII. J Immunol 177:1355–1363

  16. Lakich D, Kazazian HH, Antonarakis SE, Gitschier J (1993) Inversions disrupting the factor VIII gene are a common cause of severe haemophilia A. Nat Genet 5:236–241

  17. Livnat T, Barg AA, Levy-Mendelovich S, Kenet G (2017) Rare bleeding disorders-old diseases in the era of novel options for therapy. Blood Cells Mol Dis 67:63–68

  18. Mackman N, Tilley RE, Key NS (2007) Role of the extrinsic pathway of blood coagulation in hemostasis and thrombosis. Arterioscler Thromb Vasc Biol 27:1687–1693

  19. Manno CS, Chew AJ, Hutchison S, Larson PJ, Herzog RW, Arruda VR et al (2003) AAV-mediated factor IX gene transfer to skeletal muscle in patients with severe hemophilia B. Blood 101:2963–2972

  20. Moreau M, Feuilloley MG, Veron W, Meylheuc T, Chevalier S, Brisset LJ et al (2007) Gliding arc discharge in the potato pathogen Erwinia carotovora subsp. atroseptica: mechanism of lethal action and effect on membrane-associated molecules. Appl Environ Microbiol 73:5904–5910

  21. Mortazavi SM, Hosseinzadeh Colagar A, Sohbatzadeh F (2016) The efficiency of the cold argon-oxygen plasma jet to reduce Escherichia coli and Streptococcus pyogenes from solid and liquid ambient. IJMM 10:19–30

  22. Pinto P, Ghosh K, Shetty S (2014) Analysis of F8 inversions as risk factors for FVIII inhibitor development in Indian severe haemophilia A patients. Blood Cells Mol Dis 53:161–163

  23. Sensenig R, Kalghatgi S, Cerchar E, Fridman G, Shereshevsky A, Torabi B et al (2011) Non-thermal plasma induces apoptosis in melanoma cells via production of intracellular reactive oxygen species. Ann Biomed Eng 39:674–687

  24. Sohbatzadeh F, Hosseinzadeh Colagar A, Mirzanejhad S, Mahmodi S (2010) E. coli, P. aeruginosa, and B. cereus bacteria sterilization using afterglow of non-thermal plasma at atmospheric pressure. Appl Biochem Biotechnol 160:1978–1984

  25. Taniyama Y, Griendling KK (2003) Reactive oxygen species in the vasculature: molecular and cellular mechanisms. Hypertension 42:1075–1081

  26. Venezia RA, Orrico M, Houston E, Yin S, Naumova YY (2008) Lethal activity of nonthermal plasma sterilization against microorganisms. Infect Control Hosp Epidemiol 29:430–436

  27. Walk RM, Snyder JA, Srinivasan P, Kirsch J, Diaz SO, Blanco FC et al (2013) Cold atmospheric plasma for the ablative treatment of neuroblastoma. J Pediatr Surg 48:67–73

  28. Yada K, Nogami K, Takeyama M, Ogiwara K, Wakabayashi H, Shima M (2015) Mild hemophilia A patient with novel Pro1809Leu mutation develops an anti-C2 antibody inhibiting allogeneic but not autologous factor VIII activity. J Thromb Haemost 13:1843–1853

  29. Yang L, Wang Y, Zhou J, Cheng X, Hao X, Xie H et al (2016) Identification of genetic defects underlying FXII deficiency in four unrelated Chinese patients. Acta Haematol 135:238–240

  30. Zanetta L, Marcus SG, Vasile J, Dobryansky M, Cohen H, Eng K et al (2000) Expression of von Willebrand factor, an endothelial cell marker, is up-regulated by angiogenesis factors: a potential method for objective assessment of tumor angiogenesis. Int J Cancer 85:281–288

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Acknowledgements

We appreciate all the colleagues in the hemophilia center at Bu Ali Sina Hospital (Sari, Iran), who collaborated with us in this study. We would like to thank all the healthy and patient individuals who donated to us the blood samples, generously. We are grateful to our colleagues for critical reviews of the manuscript. This study was supported by a grant from the University of Mazandaran, dedicated to the MSc thesis of Elham Hasheminasabgorji (Registered No: 1394.11.03).

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Correspondence to Abasalt Hosseinzadeh Colagar.

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The authors declare that they have no conflict of interest.

Human and Animal Rights

The study protocol was approved by the ethics committee of the University of Mazandaran (#UMZ.REC.396006) and conducted in accordance with the Iran National Committee for Ethics in Biomedical Researches.

Informed Consent

Informed consent was obtained from all subjects.

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Hosseinzadeh Colagar, A., Hasheminasabgorji, E., Mohadjerani, M. et al. APPJ Discharge Effects on Human Factor VIII:Ag Level in Healthy and Hemophilia A Patient. Iran J Sci Technol Trans Sci 44, 13–19 (2020). https://doi.org/10.1007/s40995-019-00809-y

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Keywords

  • Factor VIII
  • Coagulation
  • Hemophilia A
  • Fibrin clot formation
  • APPJ discharge